Abstract: A set of machines and related systems build structures by the additive assembly of discrete parts. These digital material assemblies constrain the constituent parts to a discrete set of possible positions and orientations. In doing so, the structures exhibit many of the properties inherent in digital communication such as error correction, fault tolerance and allow the assembly of precise structures with comparatively imprecise tools. Assembly of discrete cellular lattices by a Modular Isotropic Lattice Extruder System (MILES) is implemented by pulling strings of lattice elements through a forming die that enforces geometry constraints that lock the elements into a rigid structure that can then be pushed against and extruded out of the die as an assembled, load bearing structure.

Abstract: A method of forming a structural honeycomb includes cutting and folding a substrate sheet according to predetermined cutting and folding patterns and fold angles that cause the sheet to form a honeycomb having cells that each have at least one face abutting, or nearly abutting, the face of another cell. The honeycomb is then stabilized by joining abutting, or nearly abutting, faces to hold the honeycomb together. The honeycomb may have a prespecified three-dimensional shape. The folding pattern may include corrugation, canted corrugation, or zig-zag folds. Joining may employ fixed and/or reversible joinery, including slotted cross section, tabbed strip, angled strip, integral skin, sewn, or laced. At least some folds may be partially-closed to create bends and twists in the honeycomb structure. Some surfaces of the honeycomb may be covered with a skin or face sheet. The substrate sheet may have flexible electronic traces.

Abstract: A machine that is capable of assembling a copy of itself from a feedstock of parts is described. The machine operates on a lattice or grid on which it is able to move and from which it receives power and control signals. The machine (assembler) is composed of modules that each perform some functionality. In the simplest case, only three module types are needed: a linear step module, a gripper, and an anchor. The linear step module is capable of moving from one lattice location to the next, the gripper module is capable of gripping other modules, and the anchor module is capable of attaching the machine to the grid. With these three primitives it is possible for this simple machine to move on the grid using inchworm-like motions, pick up other modules, and assemble a copy of itself.

Abstract: A set of machines and related systems build structures by the additive assembly of discrete parts. These digital material assemblies constrain the constituent parts to a discrete set of possible positions and orientations. In doing so, the structures exhibit many of the properties inherent in digital communication such as error correction, fault tolerance and allow the assembly of precise structures with comparatively imprecise tools. Assembly of discrete cellular lattices by a Modular Isotropic Lattice Extruder System (MILES) is implemented by pulling strings of lattice elements through a forming die that enforces geometry constraints that lock the elements into a rigid structure that can then be pushed against and extruded out of the die as an assembled, loadbearing structure.

Abstract: A machine that is capable of assembling a copy of itself from a feedstock of parts is described. The machine operates on a lattice or grid on which it is able to move and from which it receives power and control signals. The machine (assembler) is composed of modules that each perform some functionality. In the simplest case, only three module types are needed: a linear step module, a gripper, and an anchor. The linear step module is capable of moving from one lattice location to the next, the gripper module is capable of gripping other modules, and the anchor module is capable of attaching the machine to the grid. With these three primitives it is possible for this simple machine to move on the grid using inchworm-like motions, pick up other modules, and assemble a copy of itself.

Abstract: An electropermanent linear actuator has a stator, forcer, drive circuitry, and feedback control mechanism. The stator includes at least one electropermanent magnet with a coil that passes current pulses that change the magnetization of the magnet, which change persists after current is removed. The forcer moves with respect to the stator in response to the persistent changes in magnetization. Drive circuitry controls the position or speed of the actuator by controlling the timing, magnitude, and/or shape of the current pulses. The voltage and duration of pulses are of sufficient magnitude to cause the magnetization change to persist after cessation of current, with voltage and current returning substantially to zero between pulses. The feedback control mechanism determines, based on actuator velocity or position, when the next current pulse should be issued, pulse issuance being timed so that the actuator will continue to move throughout the absence of applied current between pulses.

Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.

Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.

Abstract: A process for producing a composite part includes (a) applying a loose carbon filament to a receiving portion of a first mold piece; (b) reversibly coupling the first mold piece with at least a second mold piece to form a first mold layer, wherein an interior region of the first mold layer includes a pocket configured to receive a curable resin, the pocket having a shape of the composite part; (c) infusing the curable resin into the pocket; and (d) curing the resin to form the composite part.

Abstract: An electropermanent linear actuator has a stator, forcer, drive circuitry, and feedback control mechanism. The stator includes at least one electropermanent magnet with a coil that passes current pulses that change the magnetization of the magnet, which change persists after current is removed. The forcer moves with respect to the stator in response to the persistent changes in magnetization. Drive circuitry controls the position or speed of the actuator by controlling the timing, magnitude, and/or shape of the current pulses. The voltage and duration of pulses are of sufficient magnitude to cause the magnetization change to persist after cessation of current, with voltage and current returning substantially to zero between pulses. The feedback control mechanism determines, based on actuator velocity or position, when the next current pulse should be issued, pulse issuance being timed so that the actuator will continue to move throughout the absence of applied current between pulses.

Abstract: A trans-disciplinary system for cell-free biosynthesis includes a cell-free transcription-translation (TX-TL) tool and modular, generalizable microfluidic architectures. Both components of the system are independently functional and are combinable into a cell-free biosynthesis platform. In the first component, modular plasmid libraries are used to program bacterial cell-free TX-TL systems. Each plasmid holds one gene or operon, and all the genes are controlled by the same promoter, so that the stoichiometry of enzyme synthesis is determined by the stoichiometry of plasmids in the reaction. In the second part, in order to facilitate high throughput mixing and matching of gene units from the modular plasmid libraries, a modular, reconfigurable, flexible, and scalable microfluidic architecture is employed.

Abstract: In exemplary implementations of this invention, hierarchical, nanometer-precise assembly is performed: A first structural unit is attached to a solid substrate in a first fluidic flow. A second structural unit is attached to the first structural unit in a second fluidic flow, a third structural unit is attached to the second structural unit in a third fluidic flow, and so on, until a target structure comprising the structural units is assembled. The first, second, third and so on fluidic flows are separate and occur in order in a temporal sequence. During the temporal sequence, a specific permutation of nucleobases is used repeatedly, in separate fluidic flows which occur at different times, to form multiple attachments between structural units in an assembly. The assembled target structure is removed from the solid substrate. Attachments between the structural units may be formed by nucleobase pairing.

Abstract: In exemplary implementations of this invention, a digital material comprising many discrete units is used to fabricate a sparse structure. The units are reversibly joined by elastic connections. Each unit comprises fiber-reinforced composite material. Each unit is small compared to the sparse structure as a whole. Likewise, in a sparse structure made from this digital material, the number of types of units is small compared to the total number of units. The digital material is anisotropic. This anisotropy may be due to different fiber orientations within each unit. Furthermore, different units in a single sparse structure may be oriented in different directions and in different, non-parallel planes. In some cases, the digital material is reinforced with carbon fibers, and connections between units are stronger than the units themselves. The small discrete units may be assembled into a strong, lightweight sparse structure, such as an airframe.

Abstract: In exemplary implementations, transplantation of nucleic acids into cells occurs in microfluidic chambers. The nucleic acids may be large nucleic acid molecules with more than 100 kbp. In some cases, the microfluidic chambers have only one orifice that opens to a flow channel. In some cases, flow through a microfluidic chamber temporarily ceases due to closing one or more valves. Transplantation occurs during a period in which the contents of the chambers are shielded from shear forces. Diffusion, centrifugation, suction from a vacuum channel, or dead-end loading may be used to move cells or buffers into the chambers.

Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.

Abstract: Discrete motion systems move relative to a lattice, using bistable mechanisms to snap between lattice locations. A discrete motion system includes a lattice having a regular configuration of attachment points, one or more motion modules that move across the lattice in discrete increments, and controllers that direct the modules. A module includes a body, actuators, and feet having mechanisms for attaching and detaching the module from the lattice. The module may include actuated joints that cause movement of arm structures to engage and disengage the feet from the lattice. The module may be a digital inchworm, and may be a relative assembler having at least one assembler arm. A method for discrete extensible construction includes creating a lattice having a regular configuration of attachment points, causing a discrete motion relative assembler to systematically move across the lattice in discrete increments, and causing placement of materials by the assembler arm.

Abstract: Flexural digital materials are discrete parts that can be assembled into a lattice structure to produce an actuatable structure capable of coordinated reversible spatially-distributed deformation. The structure comprises a set of discrete flexural digital material units assembled according to a lattice geometry, with a majority of the discrete units being connected, or adapted to be connected, to at least two other units according to the geometry. In response to certain types of loading of the structure, a coordinated reversible spatially-distributed deformation of at least part of the structure occurs. The deformation of the structure is due to the shape or material composition of the discrete units, the configuration of connections between the units, and/or the configuration of the lattice geometry. Exemplary types of such actuatable structures include airplane wing sections and robotic leg structures.

Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.

Abstract: A method for implementing a logic operation employs an all fluid-based no-moving part micro-mechanical logic family of microfluidic bubble logic devices that are constructed from complex sequences of microfluidic channels, microfluidic bubble modulators for programming the devices, and microfluidic droplet/bubble memory elements for chemical storage and retrieval. The input is a sequence of bubbles/droplets encoding information, with the output being another sequence of bubbles/droplets. For performing a set of reactions/tasks, the modulators program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the microfluidic channel sequence, utilizing the generated bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.

Abstract: A family of reconfigurable asynchronous logic elements that interact with their nearest neighbors permits reconfigurable implementation of circuits that are asynchronous at the bit level. A reconfigurable asynchronous logic cell comprises a set of one-bit buffers for communication with at least one neighboring cell, each buffer capable of having several states and configured for receiving input state tokens from neighboring cells and for transferring output state tokens to neighboring cells, and a one-bit processor configured to perform a logic operation utilizing received tokens as inputs and to produce an output token reflecting the result of the logic operation, wherein the logic operation and the functional configuration of the buffers are reconfigurably programmable. A reconfigurable logic circuit comprises a plurality of reconfigurable logic cells that compute by locally passing state tokens and are reconfigured by the directed shifting of programming instructions through neighboring logic cells.