Abstract: Detection apparatus for use in the detection of the presence of a selected pathogen in a sample are disclosed. Such apparatus include: a substrate with a detection region on a surface thereof, the detection region having microstructures including grooves formed therein that will align liquid crystal material in contact therewith, the width and depth of the grooves being in the range of 10 &mgr;m or less; a blocking layer on the surface of the detection region of the substrate that does not disrupt the alignment of liquid crystal material in contact therewith, the blocking layer blocking nonspecific adsorption of pathogens to the surface; and a binding agent on the surface of the detection region of the substrate, the binding agent specifically binding the selected pathogen.

Abstract: A method for preparing a rubbed substrate structure suitable for use in a liquid crystal assay device, includes reacting a biochemical blocking compound that includes at least one reactive group with an activated modified surface of a support, the activated modified surface of the support having at least one functional group capable of reacting with the reactive group of the biochemical blocking compound, wherein a covalent bond is formed between the biochemical blocking compound and the support producing a support with a surface comprising the biochemical blocking compound. The method also includes rubbing the surface having the biochemical blocking compound to produce a rubbed surface that possesses features that drive uniform anchoring of liquid crystals when the liquid crystals contact the rubbed surface.

Abstract: A method for preparing a metallized surface that possesses gradients in surface topography includes obliquely depositing a metal from a metal source onto a surface of a support. The surface of the support includes a first end, a second end, and a region between the first and second ends. The second end of the surface is located nearer to the metal source than is the first end, and the metal is deposited onto the first end of the surface at a first angle of incidence and the metal is deposited onto the second end of the surface at a second angle of incidence. The first angle of incidence is greater than the second angle of incidence, and the metal is deposited onto the region between the first and second ends at angles of incidence that vary over the region to produce the metallized surface with gradients in surface topography. The angles of incidence are measured from the normal of the support.

Abstract: A rubbed substrate structure for use in a liquid crystal assay device, includes: a biochemical blocking compound chemically immobilized on a surface of one side of a support forming a biochemical blocking layer; and a biomolecule recognition agent deposited on the side of the support containing the biochemical blocking layer. The biomolecule recognition agent includes a recognition site capable of selectively recognizing a target species to be detected by the liquid crystal assay device. Additionally, the surface of the side of the support containing the biochemical blocking layer is rubbed such that it possesses features that drive a uniform anchoring of liquid crystals when the liquid crystals contact the side of the support containing the biochemical blocking layer.

Abstract: A printing apparatus in the form of an inkjet printer and a printing method utilizes a light-activated ink release system. The apparatus includes at least one nozzle having an ink body that is comprised of light-sensitive ink. The ink can form an unextended droplet meniscus. By directing a light beam onto the unextended droplet meniscus, the surface tension of the light-sensitive ink decreases, and provides for an extended droplet meniscus. The formation of the extended meniscus droplet meniscus permits the transfer of ink to a receiver or media.

Abstract: The present invention provides a novel class of water-soluble cleavable surfactants and methods for utilizing these surfactants.

Abstract: A DNA hybridization surface includes a support having a self assembled monolayer on a metallized surface. The self assembled monolayer includes an alkanethiol and a strand of nucleic acids comprising a functional group that binds to the metallized surface. A method for detecting DNA hybridization in a sample includes (a) incubating a DNA hybridization surface with an aqueous sample that includes a fragment of DNA to produce an incubated DNA hybridization surface; (b) rinsing the incubated DNA hybridization surface to produce a rinsed incubated DNA hybridization surface; (c) contacting the rinsed incubated DNA hybridization surface with a liquid crystal; and (d) determining whether the liquid crystal is uniformly anchored on the rinsed incubated DNA hybridization surface.

Abstract: A device for detecting a compound in a sample includes a substrate and a self-assembled monolayer. The substrate includes a support with a metallized top surface, and the self-assembled monolayer includes an alkanethiol attached to the metallized top surface of the substrate and having a functional group that reversibly or irreversibly interacts with the compound. A liquid crystal is disposed on the self-assembled monolayer opposite the side of the self-assembled monolayer attached to the metallized top surface of the substrate. The liquid crystal includes a moiety that interacts with the functional group of the alkanethiol. When the compound is present in a sample that that contacts the self-assembled monolayer, the orientation of the liquid crystal is altered.

Abstract: Interactions between molecules which are components of self-assembled monolayers and other molecules can be amplified and transduced into an optical signal through the use of a mesogenic layer. The invention provides a device and methods for detecting analytes. The device comprises a substrate onto which a self-assembled monolayer is attached and a mesogenic layer which is anchored by the self-assembled monolayer. The mesogenic layer undergoes a change in conformation in response to the molecular interaction.

Abstract: A printing apparatus in the form of an inkjet printer and a printing method utilizes a light-activated ink release system. The apparatus includes at least one nozzle having an ink body that is comprised of light-sensitive ink. The ink can form an unextended droplet meniscus. By directing a light beam onto the unextended droplet meniscus, the surface tension of the light-sensitive ink decreases, and provides for an extended droplet meniscus. The formation of the extended meniscus droplet meniscus permits the transfer of ink to a receiver or media.

Abstract: A rubbed substrate structure for use in a liquid crystal assay device, includes: a biochemical blocking compound chemically immobilized on a surface of one side of a support forming a biochemical blocking layer; and a biomolecule recognition agent deposited on the side of the support containing the biochemical blocking layer. The biomolecule recognition agent includes a recognition site capable of selectively recognizing a target species to be detected by the liquid crystal assay device. Additionally, the surface of the side of the support containing the biochemical blocking layer is rubbed such that it possesses features that drive a uniform anchoring of liquid crystals when the liquid crystals contact the side of the support containing the biochemical blocking layer.

Abstract: A printing apparatus in the form of an inkjet printer and a printing method utilizes a light-activated ink release system. The apparatus includes at least one nozzle having an ink body that is comprised of light-sensitive ink. The ink can form an unextended droplet meniscus. By directing a light beam onto the unextended droplet meniscus, the surface tension of the light-sensitive ink decreases, and provides for an extended droplet meniscus. The formation of the extended meniscus droplet meniscus permits the transfer of ink to a receiver or media.

Abstract: Detection apparatus for use in the detection of the presence of a selected pathogen in a sample are disclosed. Such apparatus include: a substrate with a detection region on a surface thereof, the detection region having microstructures including grooves formed therein that will align liquid crystal material in contact therewith, the width and depth of the grooves being in the range of 10 &mgr;m or less; a blocking layer on the surface of the detection region of the substrate that does not disrupt the alignment of liquid crystal material in contact therewith, the blocking layer blocking nonspecific adsorption of pathogens to the surface; and a binding agent on the surface of the detection region of the substrate, the binding agent specifically binding the selected pathogen.

Abstract: Interactions between molecules which are components of self-assembled monolayers and other molecules can be amplified and transduced into an optical signal through the use of a mesogenic layer. The invention provides a device and methods for detecting analytes. The device comprises a substrate onto which a self-assembled monolayer is attached and a mesogenic layer which is anchored by the self-assembled monolayer. The mesogenic layer undergoes a change in conformation in response to the molecular interaction.

Abstract: Scanning probe microscopy is used to quantitatively characterize structural anisotropy within obliquely deposited metal films. Whereas visual inspection of AFM images (real space or reciprocal space) reveals no obvious structural anisotropy within these gold films, by quantitative analysis of the AFM profiles, subtle structural anisotropy is observed. The quantitative characterization provides a method to estimate the influence of anisotropy on the orientations of supported mesogenic layers.

Abstract: Interactions between molecules which are components of self-assembled monolayers and other molecules can be amplified and transduced into an optical signal through the use of a mesogenic layer. The invention provides a device and methods for detecting analytes. The device comprises a substrate onto which a self-assembled monolayer is attached and a mesogenic layer which is anchored by the self-assembled monolayer. The mesogenic layer undergoes a change in conformation in response to the molecular interaction.

Abstract: A coplanar waveguide polymeric in-line fiber construct (CPW-PILF) formed on an optic half coupler substrate base or D-fiber wherein the surface is polished down through the cladding on the optical fiber so as to form an evanescent coupling region on the surface. Co-planar, spaced-apart electrodes are deposited on the surface with their gap aligned over the coupling region, and an electro-optic (EO) polymeric waveguide is deposited over the electrodes and between the electrode gap. Light transmitted thorough the optical fiber is evanescently coupled to said waveguide and modulated by a signal applied to the electrodes. Alternatively, the waveguide is deposited on the surface of the substrate and the electrodes are deposited over the waveguide.

Abstract: A coplanar waveguide polymeric in-line fiber construct (CPW-PILF) formed on an optic half coupler substrate base or D-fiber wherein the surface is polished down through the cladding on the optical fiber so as to form an evanescent coupling region on the surface. Co-planar, spaced-apart electrodes are deposited on the surface with their gap aligned over the coupling region, and an electro-optic (EO) polymeric waveguide is deposited over the electrodes and between the electrode gap. Light transmitted thorough the optical fiber is evanescently coupled to said waveguide and modulated by a signal applied to the electrodes. Alternatively, the waveguide is deposited on the surface of the substrate and the electrodes are deposited over the waveguide.