Abstract: A microbe quantifying apparatus includes: a filtering mechanism that filters microbes contained in a fluid sample with a filter; a quantifying mechanism that quantifies the microbes with a specified biological material contained in the microbes on the filter as an index; and an anti-drying mechanism that prevents drying of the microbes on the filter.

Abstract: Provided is a device for collecting biological material from a microbe, the device including: a collection container that is used to collect a microbe from a liquid sample containing the microbe and to extract the biological material from the microbe; and a temperature control mechanism that controls a temperature of the collection container. Herein, the temperature control mechanism controls a temperature of the collection container so that the microbe is collected at a first temperature and the biological material is extracted from the microbe at a second temperature higher than the first temperature.

Abstract: A microorganism measuring system set control reference values with higher accuracy than ever. Based on results of repeated CFU countings and results of repeated ATP measurements, a microorganism measuring system obtains a probability density function of ATP measured values in a normal condition; determines an alert reference value at which a desired probability of false positives is equal to or less than a probability, and an action reference value at which a desired probability of false negatives is equal to or less than a probability; and thereby controls the ATP measured values. For this reason, the microorganism measuring system can set the control reference values with higher accuracy than ever, without being influenced by an error in the conversion of ATP contents into CFU counts.

Abstract: Provided is a method for producing an instrument of collecting detection target. The instrument includes a filter unit and a carrier unit that holds a carrier of collecting a detection target. The method comprises at least: a tool ATP eliminating step of eliminating ATP from tools to be used in the production steps, a mixture ATP eliminating step of eliminating ATP from a carrier prepared by mixing raw materials via using the tools from which ATP is thus eliminated, and a filling step of filling the carrier from which ATP is thus eliminated into a carrier unit.

Abstract: An object of the present invention is to provide an object-capturing device which accurately detects objects such as microorganisms captured at a test site. The object-capturing device of the present invention includes a capturing dish holding a carrier, which captures objects (microorganisms), on a first side of the capturing dish. The capturing dish has a through hole extending from the first side to a second side of the capturing dish. The object-capturing device is used in a way such that, after the objects are captured with the carrier directed upward, the carrier is directed downward and reagents for detecting the objects are contacted with the objects captured on the carrier through the through hole.

Abstract: A collection unit (80)includes: a collection carrier cartridge (82) formed, at its center, with a through hole (82b3) into which a nozzle for supplying hot water or ATP reagent is inserted including a carrier filling dish (82b), on an outer circumference of the through hole (82b3), to be filled with a collection carrier (90) for collecting floating bacteria in the air, and an upper lid (82a) on which the carrier filling dish (82b) is placed, formed with a protrusion to be inserted through the through hole (82b3); an impactor nozzle head (86) covering a surface of the collection carrier (90) and has a plurality of nozzle holes (87) facing the collection carrier (90) surface; and a fan (84) introducing air to the collection carrier surface through the nozzle holes (87). A velocity of the air passing through the nozzle holes (87) is 40 m/s to 50 m/s.

Abstract: A device for quantifying biological material (ATP) of cells contained in a liquid sample as a sample is provided. The device includes a controller that calculates an amount of the biological material of cells contained in the liquid sample, based on a differential amount of luminescence between an amount of luminescence when an ATP luminescence reagent as a luminescent reagent for biological material is reacted with the biological material (ATP) that is separated and extracted from cells contained in the liquid sample by bringing an ATP extraction reagent as a processing reagent into contact with the liquid sample, and an amount of luminescence when the ATP luminescent reagent is reacted with the ATP extraction reagent.

Abstract: Provided is a device for collecting biological material from a microbe, the device including: a collection container that is used to collect a microbe from a liquid sample containing the microbe and to extract the biological material from the microbe; and a temperature control mechanism that controls a temperature of the collection container. Herein, the temperature control mechanism controls a temperature of the collection container so that the microbe is collected at a first temperature and the biological material is extracted from the microbe at a second temperature higher than the first temperature.

Abstract: A microbe quantifying apparatus includes: a filtering mechanism that filters microbes contained in a fluid sample with a filter; a quantifying mechanism that quantifies the microbes with a specified biological material contained in the microbes on the filter as an index; and an anti-drying mechanism that prevents drying of the microbes on the filter.

Abstract: The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.

Abstract: The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.

Abstract: In a microorganism capturing device as a filter separation apparatus, a first contact chamber and a second contact chamber in each of which an object to be treated is contacted with a treatment agent are disposed apart from each other by a hydrophilic filter. The second contact chamber and a filtrate outlet are separated from each other by a hydrophobic filter. The microorganism capturing device is adapted to efficiently contact the object to be treated with the treatment agent, even if the object to be treated enters into the hydrophilic filter.

Abstract: The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.

Abstract: A reagent splitting/dispensing method based on a reagent dispensing nozzle controls a split or dispensation amount of reagent, includes a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle; a first moving process of moving the reagent dispensing nozzle to a position above the reagent to be split; a second moving process of depositing the nozzle tip end in the reagent; an air layer adjusting process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and decreasing the occupation amount of the first air layer, between the first moving process and the second moving process; and a reagent splitting process of decreasing the occupation amount of the operation fluid in the reagent dispensing nozzle and filling the reagent into the reagent dispensing nozzle from the nozzle tip end.

Abstract: Provided is a device for capturing object, wherein a carrier which captures substances to be detected and is made to be dividable into plural portions, is placed with the plural dividable portions arranged in sections. The carrier includes a count analysis carrier and an identification analysis carrier respectively arranged in sections in a first dish half and a second dish half which are obtained by dividing a capturing dish.

Abstract: A collection unit (80) of this invention includes: a collection carrier cartridge (82) formed, at its center, with a through hole (82b3) into which a nozzle for supplying hot water or ATP reagent is inserted including a carrier filling dish (82b), on an outer circumference of the through hole (82b3), to be filled with a collection carrier (90) for collecting floating bacteria in the air and an upper lid (82a) on which the carrier filling dish (82b) is placed, formed with a protrusion to be inserted through the through hole (82b3); an impactor nozzle head (86) which covers a surface of the collection carrier (90) and has a plurality of nozzle holes (87) facing the surface of the collection carrier (90); and a fan (84) which introduces air to the surface of the collection carrier through the nozzle holes (87). A velocity of the air passing through the nozzle holes (87) is 40 m/s to 50 m/s.

Abstract: A reagent open mechanism of the luminescence measurement system comprises a triaxial actuator and a reagent dispensing nozzle which is driven by the triaxial actuator. A reagent cartridge where a reagent to be divided by the reagent dispensing nozzle is filled in a concave and the opening of the concave is sealed by an aluminum sheet can be set in. This reagent open mechanism comprises an open needle which is driven by the triaxial actuator and makes a hole in the aluminum sheet and a fixation block between the reagent dispensing nozzle and the open needle which arranges the reagent dispensing nozzle and the open needle in such location that the reagent dispensing nozzle or the open needle does not contact with a structure including the reagent cartridge in a Z-axis operation during opening time or reagent dividing and dispensing time.

Abstract: The present invention provides for a reagent splitting/dispensing device and method that can prevent contamination of an operation fluid when a reagent dispensing nozzle is in a waiting state and prevent falling of a droplet. The reagent splitting/dispensing method includes disposing and adjusting an air layer between an interface of an operation fluid and a reagent aspirated and subsequently dispensed from a nozzle tip end in an reagent dispensing nozzle.

Abstract: A floating bacteria counting device capable of trapping floating bacteria in air continuously for a long time without decreasing trapping efficiency even if the time before a trapping carrier being replaced is prolonged and a floating bacteria counting method and a floating bacteria counting system using the floating bacteria trapping device are provided. A nozzle (16) is formed of a plurality of pinholes (16a) arranged at equal intervals. If a pitch interval between the pinholes (16a) is d, a driving mechanism (22) causes a support container (20) supporting a trapping carrier (30) to move in a horizontal direction so that the support container (20) draws a circle of less than d in diameter.