Abstract: [Problem] An object of the present invention is to provide pipe cleaning means that can effectively control introduction of viable bacteria to an inside of an apparatus and contamination occurring in the apparatus and particularly provide a pipe cleaning method and a pipe cleaning mechanism in a luminescence measurement apparatus. [Solution] A cleaning method of a pipe of a luminescence measurement apparatus (10) according to the present invention includes a bacteriolytic process of supplying a sterilization reagent into a supply pipe for hot water or a reagent in the luminescence measurement apparatus (10) and sterilizing an inside of the supply pipe, an ATP removal process of supplying an ATP removal reagent into the supply pipe after the sterilization of the inside of the supply pipe and removing ATP inside the supply pipe, and a process of replacing the ATP removal reagent in the supply pipe with pure water.

Abstract: A reagent cartridge for a microorganism detection apparatus includes a plurality of reagent vessels, a support plate, and side plates, wherein the reagent vessels are integrally connected with each other in parallel by the support plate, and a group of the plurality of the reagent vessels is surrounded by the side plates; the reagent cartridge further includes an independent reagent vessel instead of at least one of the plurality of the reagent vessels and separately therefrom; and an engagement portion, wherein the engagement portion engages the independent reagent vessel in the engagement portion so as to be universally attached and detached, and to be in parallel with the plurality of the reagent vessels.

Abstract: The present invention provides a reagent splitting/dispensing method that can prevent contamination of an operation fluid when a reagent dispensing nozzle is in a waiting state and prevent falling of a droplet.

Abstract: A reagent open mechanism of the luminescence measurement system comprises a triaxial actuator and a reagent dispensing nozzle which is driven by the triaxial actuator. A reagent cartridge where a reagent to be divided by the reagent dispensing nozzle is filled in a concave and the opening of the concave is sealed by an aluminum sheet can be set in. This reagent open mechanism comprises an open needle which is driven by the triaxial actuator and makes a hole in the aluminum sheet and a fixation block between the reagent dispensing nozzle and the open needle which arranges the reagent dispensing nozzle and the open needle in such location that the reagent dispensing nozzle or the open needle does not contact with a structure including the reagent cartridge in a Z-axis operation during opening time or reagent dividing and dispensing time.

Abstract: A mechanism for preventing suction air from leaking uses a capturing carrier cartridge that includes a cartridge main body and a filter fixing ring. The cartridge main body includes a storage portion for storing a capturing carrier solution and a filter mounting portion where a filter for filtering the capturing carrier solution is disposed. The filter fixing ring covers the filter mounting portion, positions the filter, and sucks and filters the capturing carrier solution from a lower surface thereof. The mechanism includes a cartridge holder and a suction head. The cartridge holder includes an opening that includes the filter mounting portion of the capturing carrier cartridge, and a holding portion that holds the storage portion. The suction head sucks the capturing carrier solution through the lower surface of the filter fixing ring while applying pressure to press the filter fixing ring against the filter mounting portion.

Abstract: An object of the present invention is to provide an object-capturing device which accurately detects objects such as microorganisms captured at a test site. The object-capturing device of the present invention includes a capturing dish holding a carrier, which captures objects (microorganisms), on a first side of the capturing dish. The capturing dish has a through hole extending from the first side to a second side of the capturing dish. The object-capturing device is used in a way such that, after the objects are captured with the carrier directed upward, the carrier is directed downward and reagents for detecting the objects are contacted with the objects captured on the carrier through the through hole.

Abstract: The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes.