Patents by Inventor Norman C. Nelson

Norman C. Nelson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10688458
    Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: June 23, 2020
    Assignees: Gen-Probe Incorporated, Qualigen, Inc.
    Inventors: Scott S. Breidenthal, Richard S. Lee, Norman C. Nelson, Matthew J. Scott, Jason A. Taylor
  • Patent number: 10590453
    Abstract: The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
    Type: Grant
    Filed: August 16, 2018
    Date of Patent: March 17, 2020
    Assignee: AEGEA BIOTECHNOLOGIES, INC.
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Publication number: 20200002740
    Abstract: The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
    Type: Application
    Filed: August 16, 2018
    Publication date: January 2, 2020
    Applicant: Aegea Biotechnologies, Inc.
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Patent number: 10415092
    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: September 17, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Norman C. Nelson, Lyle J. Arnold, Michael M. Becker
  • Patent number: 10407723
    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: September 10, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Norman C. Nelson, Lyle J. Arnold, Michael M. Becker
  • Patent number: 10385476
    Abstract: Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: August 20, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Jijumon Chelliserry
  • Publication number: 20190185911
    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
    Type: Application
    Filed: January 7, 2019
    Publication date: June 20, 2019
    Inventors: Norman C. NELSON, Lyle J. ARNOLD, Lizhong DAI, Steven PHELPS, Jijumon CHELLISERRY
  • Publication number: 20190185917
    Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
    Type: Application
    Filed: December 17, 2018
    Publication date: June 20, 2019
    Inventors: Norman C. NELSON, Jijumon CHELLISERRY
  • Publication number: 20190144906
    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5? to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5? to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.
    Type: Application
    Filed: November 19, 2018
    Publication date: May 16, 2019
    Inventors: Norman C. Nelson, Margarita Batranina-Kaminsky
  • Publication number: 20190126219
    Abstract: A receptacle comprises opposed members, a plurality of chambers having perimeter walls defined by seals formed between the opposed members and portals interconnecting the chambers, and a rigid frame supporting the opposed members at their peripheral edges. The frame comprises a front frame portion and a rear frame portion, and the peripheral edges of the opposed members are retained between the front and rear frame portions. An inlet port extends between the front and rear frame portions and is in fluid communication with one of the chambers.
    Type: Application
    Filed: December 27, 2018
    Publication date: May 2, 2019
    Applicants: Gen-Probe Incorporated, Qualigen, Inc.
    Inventors: Scott S. BREIDENTHAL, Richard S. Lee, Norman C. Nelson, Matthew J. Scott, Jason A. Taylor
  • Publication number: 20190126220
    Abstract: A method of processing a sample in a receptacle comprising a plurality of chambers. Each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material.
    Type: Application
    Filed: December 27, 2018
    Publication date: May 2, 2019
    Applicants: Gen-Probe Incorporated, Qualigen, Inc.
    Inventors: Scott S. BREIDENTHAL, Richard S. LEE, Norman C. NELSON, Matthew J. SCOTT, Jason A. TAYLOR
  • Publication number: 20190112625
    Abstract: The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
    Type: Application
    Filed: August 16, 2018
    Publication date: April 18, 2019
    Applicant: Aegea Biotechnologies, Inc.
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Patent number: 10214760
    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5? to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5? to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: February 26, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Margarita Batranina-Kaminsky
  • Patent number: 10202629
    Abstract: The present invention provides methods of amplifying a target nucleic acid utilizing a clamp oligonucleotide comprising a first target-binding region on the 3?-terminus and a second target-binding region on the 5?-terminus and tether region in between. The tether region may comprise a variety of user-defined sequences or elements that allow for further manipulation of the target nucleic acid. Such as, for example, capture followed by amplification, identification and/or sequencing. The target-binding regions bind to the target nucleic acid, the 3?-terminus functions as a primer to initiate extension across the target nucleic acid sequence and ligation of the gap results in formation of a circularized nucleic acid. This circular template can be used in a variety of processes, including amplification and sequencing.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: February 12, 2019
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Patent number: 10196674
    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: February 5, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Lyle J. Arnold, Jr., Lizhong Dai, Steven Phelps, Jijumon Chelliserry
  • Patent number: 10184147
    Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
    Type: Grant
    Filed: June 23, 2016
    Date of Patent: January 22, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Jijumon Chelliserry
  • Patent number: 10174352
    Abstract: The present invention provides methods for amplifying a nucleic acid from a sample containing a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification that assists in further manipulation of the target nucleic acid, such as sequencing. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. One of these methods provides a first oligonucleotide and a second oligonucleotide affixed to a solid support or separate solid supports. The first oligonucleotide is blocked to prevent extension from the 3?-terminus and has a sequence complementary to a first portion of a target nucleic acid. The second oligonucleotide has a sequence that is identical to a second portion of the target nucleic acid. In this method, a sample is applied to the solid support and the target nucleic acid within the sample binds said first oligonucleotide.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: January 8, 2019
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Patent number: 10119163
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: November 6, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Patent number: 10081825
    Abstract: The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 25, 2018
    Assignee: AEGEA BIOTECHNOLOGIES, INC.
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Patent number: 10072290
    Abstract: The present invention provides methods of amplifying a fragmented target nucleic acid containing short target nucleic acid fragments utilizing an assembler sequence to convert these short fragments into longer sequences enabling their identification and interrogation. This is particularly important when attempting to identify small genetic variations, such as SNVs, present in highly fragmented nucleic acid samples. Amplification is accomplished by hybridizing the short target nucleic acid sequences to the assembler sequence, where these short sequences serve as primers for extension. Since the fragmented target nucleic acids that contain SNVs are utilized as primers on the assembler sequence they are preserved during amplification and can be detected.
    Type: Grant
    Filed: March 15, 2014
    Date of Patent: September 11, 2018
    Assignee: AEGEA BIOTECHNOLOGIES, INC.
    Inventors: Lyle J. Arnold, Norman C. Nelson