Abstract: The invention a novel and efficient method for protoplast culture comprising the steps of isolating the protoplasts from a cell suspension, mixing the protoplasts with equal volumes of alginate solution, placing 40-50 &mgr;l of CaCl2 solution on a glass microslide, placing a mixture of protoplasts and alginate solution on the glass microslide and immediately covering by a glass coverglass, adding CaCl2 solution from the sides of coverglass, sliding down the coverglass towards one side and placing it in a petridish containing protoplast culture medium, sealing the petridishes, incubating it and transferring the extra thin alginate layer with celled colonies to regeneration medium for development of culture.

Abstract: The invention provides a one step method for micropropagation of tea plants from leaf explants via callus phase, wherein the explants are maintained in basal Murashige and Skoog medium (Murashige T. and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497; 1962) supplemented with vitamins like thiamine-HCl (0.05 to 2.0 mg/l), pyridoxine-HCl (0.25 to 1.5 mg/l) and nicotinic acid (0.25 to 1.5 mg/l) together with glycine (1.0 to 3.0 mg/l) and either 2.5, 5.0 or 7.5 mg/l 2,4-Dichlorophenoxy acetic acid for a period of 14-16 weeks, from the stage of induction of callus to rhizogenesis and shoot formation.

Abstract: The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla astrisanguinea Lodd. Var. orgyrophylla, said isozyme having the following characteristics, O2−. scavenging activity remains same before and after autoclaving; scavenges O2−. from sub-zero temperature of −20° C. to high temperature of +80° C.; O2−. scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2−. scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and/ or macro-organism after autoclaving; possesses temperature optima at 0° C.

Abstract: The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla atrosanguinea Lodd. Var. orgyrophylla, said isozyme having the following characteristics, O2− scavenging activity remains same before and after autoclaving; scavenges O2− from sub-zero temperature of −20° C. to high temperature of +80° C.; O2− scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2− scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0° C.

Abstract: The invention a novel and efficient method for protoplast culture comprising the steps of isolating the protoplasts from a cell suspension, mixing the protoplasts with equal volumes of alginate solution, placing 40-50 &mgr;l of CaCl2 solution on a glass microslide, placing a mixture of protoplasts and alginate solution on the glass microslide and immediately covering by a glass coverglass, adding CaCl2 solution from the sides of coverglass, sliding down the coverglass towards one side and placing it in a petridish containing protoplast culture medium, sealing the petridishes, incubating it and transferring the extra thin alginate layer with celled colonies to regeneration medium for development of culture.

Abstract: The present invention describes a method for inducing improved seed germination in a high altitude medicinal plant, Podophyllum hexandrum Royle through hot water treatment at 40° C.-100° C. for 30-120 seconds.

Abstract: The present invention describes a method for inducing improved seed germination in a high altitude medicinal plant, Podophyllum hexandrum Royle through hot water treatment at 40° C.-100° C. for 30-120 seconds.

Abstract: A compact, portable, lightweight, low power consuming, convenient, versatile and sterile laminar airflow device, useful in obtaining a workspace substantially devoid of airborne particulate contaminants, said device comprising a body (C) divided into an upper and lower chambers; the upper chamber housing one or more pre-filtration members (B), a motor (T) driving a fan (S), and one or more filters (U) located below the motor; and the lower chamber provided with a slideable front panel (M), a removable platform (X) located at the lower portion of the chamber and a perforated plane (N) placed on the removable platform.