Abstract: Azlactone-functional polymer supports are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biologically active supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional supports have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.2 and R.sup.3 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms, andn is an integer 0 or 1, the azlactone functional supports having 0.

Abstract: Graft copolymers comprising a poly-alpha-olefin base polymer selected from the group consisting of polyethylene, polypropylene, polystyrene, and compatible mixtures thereof, having grafted thereto an olefinic monomer. The grafted monomer is present in an amount effective to increase the amount of protein that will bind to the graft copolymer as compared with the base polymer.Also disclosed are polymer/protein compositions comprising a graft copolymer having a protein immobilized on the surface thereof, processes for the preparation of the above-described graft copolymers and compositions, methods of immobilizing proteins, and methods of immunoassay based on such immobilization.

Abstract: Supports having azlactone-functional surfaces, adduct supports prepared from such azlactone-functional supports, and methods of preparing both are disclosed. Azlactone functionality is introduced to surfaces of a pre-existing support in a manner which retains useful physical and chemical characteristics of the pre-existing support. One method involves exposing surfaces with high energy radiation to generate free radical reaction sites on the surfaces and causing azlactone-functional moieties to react with the free radical reaction sites. Another method involves coating surfaces with azlactone monomers, crosslinking monomers, and optionally co-monomers and polymerizing the monomers to form a polymerized coating of azlactone-functionality on the surfaces. Another method involves dispersion polymerization of azlactone-functional moieties to produce azlactone-functional particles within pores and interstices of a pre-existing support.

Abstract: Azlactone-functional polymer supports are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biologically active supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional supports have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.2 and R.sup.3 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms, andn is an integer 0 or 1,the azlactone functional supports having 0.

Abstract: Azlactone-functional polymer supports are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biologically active supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional supports have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.2 and R.sup.3 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms, andn is an integer 0 or 1,the azlactone functional supports having 0.

Abstract: Graft copolymers and processes for the preparation thereof are provided. The copolymers comprise a base polymer having grafted thereto a monomeric 2-alkenyl azlactone. The surface properties of the graft copolymers can be modified by binding thereto nucleophilic reagents comprising further functional groups with desired properties. Further, the amount of azlactone available at the surface of a graft copolymer for binding to such nucleophilic reagents can be controlled by selecting a surface against which the graft copolymer is formed. The graft copolymers exhibit desirable thermoplastic, melt flow, and adhesion properties and are particularly useful for immobilizing proteins. Methods of immunoassay based on the immobilization of proteins are also disclosed. Another utility of the graft copolymers involves the compatibilizing of immiscible polymer blends.

Abstract: The present invention provides a stable stationary phase for chromatography which comprises porous ZrO.sub.2 spherules coated with a cross-linked polymer coating wherein said coated spherules have a pore size from about 20-500 .ANG. and a particle diameter range of about 0.5-500 microns, and are stable in basic media to pHs of up to about 14.

Abstract: A biologically active adduct support, prepared from an azlactone-functional polymeric support and biomolecules covalently immobilized thereon, is disclosed. The biomolecules are covalently immobilized in the presence of polyanionic salt using a method which achieves both high density and optimized bound specific biological activity. Preferably, the immobilization occurs in the presence of an azlactone quencher.

Abstract: The present invention provides a stable stationary phase for chromatography which comprises porous ZrO.sub.2 spherules coated with a cross-linked polymer coating wherein said coated spherules have a pore size from about 20-500 .ANG. and a particle diameter range of about 0.5-500 microns, and are stable in basic media to pHs of up to about 14.

Abstract: The present invention provides a stable stationary phase for chromatography which comprises porous ZrO.sub.2 spherules coated with a cross-linked polymer coating wherein said coated spherules have a pore size from about 20-500 .ANG. and a particle diameter range of about 0.5-500 microns, and are stable in basic media to pHs of up to about 14.

Abstract: A graft copolymer is provided. The copolymer comprises a poly-alpha-olefin base polymer that does not undergo substantial crosslinking under polymer melt conditions in the presence of a free radical initiator, having grafted thereto a monomeric 2-alkenyl azlactone. The graft copolymers exhibit desirable thermoplastic, melt flow, and adhesion properties and are particularly useful for immobilizing proteins.

Abstract: Azlactone-functional polymer beads are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biomacromolecule-bearing supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional beads have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.2 and R.sup.

Abstract: Azlactone-functional polymer beads are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, reagents, and as enzyme or other protein-bearing supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional beads have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.1 and R.sup.3 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms,andn is an integer 0 or 1.

Abstract: There is provided a group of tripeptide thiol ester substrates of the following generalized structure:B--AA.sub.3 --AA.sub.2 --AA.sub.1 --S--R.These substrates yield, upon enzymatic cleavage of the thiol ester bond, thiols which can be quantified accurately through certain coupling reactions. The substrates are useful for the quantitative determination of proteolytic enzymes in biological fluids.