Abstract: A novel gene defining a novel enzyme UDP-galactose: ?-D-galactose-R 4-?-D-galactosyltransferase, termed ?4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding ?4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting ?4Gal-T1 activity, as well as cloning and expression vectors including such DNA, host cells comprising DNA encoding ?4Gal-T1, and recombinant methods for providing ?4Gal-T1. The enzyme ?4Gal-T1 and ?4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining ?1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active ?4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active ?4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids.

Abstract: A novel gene defining a novel enzyme UDP-galactose: ?-D-galactose-R 4-?-D-galactosyltransferase, termed ?4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding ?4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting ?4Gal-T1 activity, as well as cloning and expression vectors including such DNA, host cells comprising DNA encoding ?4Gal-T1, and recombinant methods for providing ?4Gal-T1. The enzyme ?4Gal-T1 and ?4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining ?1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active ?4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active ?4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids.

Abstract: An articulated hinge is provided which is capable of supporting a panel member and permitting the panel member to be moved away from an opening and at the same time permit the panel member to be rotated approximately 90 degrees. The hinge has a scissor arm mechanism which provides for smooth opening and closing of the hinge thereby eliminating a possible sticking of the hinge during opening.

Abstract: The present invention features compositions and methods related to truncated mutants of ST6GalNAcI. In particular, the invention features truncated human, mouse, and chicken ST6GalNAcI polypeptides. The invention also features nucleic acids encoding such truncated polypeptides, as well as vectors, host cells, expression systems, and methods of expressing and using such polypeptides.

Abstract: A system, process and computer software is disclosed for electronically accessing financial terms related to the acquisition of a vehicle by a purchaser, including contact information, original vehicle information, and the settlement amount. This system may electronically accesses used vehicle valuations, including an estimate for a vehicle similar to the vehicle included with the financial terms. The system may automatically correlate the financial terms to the used vehicle valuations. The system may determine the value-difference between the financial terms of the original vehicle and a new vehicle. The system may transmit a message to the purchaser to communicate that the purchaser can replace the original vehicle with a new vehicle.

Abstract: A novel gene defining a novel enzyme in the UDP-D-galactose: b-N-acetyl-glucosamine ?-1,4-galactosyltransferase family, termed ?4Gal-T2, with unique enzymatic properties is disclosed. The enzymatic activity of ?4Gal-T2 is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs encoding ?4Gal-T2 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting ?4Gal-T2 activity, as well as cloning and expression vectors including such DNA, cells transfected with the vectors, and recombinant methods for providing ?4Gal-T2. The enzyme ?4Gal-T2 and ?4Gal-T2-active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of ?4Gal-T2.

Abstract: Attachment of O-glycans to proteins is controlled by a large family of homologous polypeptide GalNAc-transferases. Polypeptide GalNAc-transferases contain a C-terminal sequence with similarity to lectins. This invention discloses that the putative lectin domains of GalNAc-transferase isoforms, GalNAc-T4, -T7, -T2, and -T3, are functional and recognize carbohydrates, glycopeptides, and peptides and discloses the lectin domains of GalNAc-T1-T16. These lectin domains have different binding specificities and modulate the functions of GalNAc-transferase isoforms differently. Novel methods for identification of inhibitors or modulators of binding activities mediated by lectin domains of polypeptide GalNAc-transferases are disclosed. Direct binding activity of GalNAc-transferase lectins has been demonstrated for the first time and methods to measure lectin mediated binding of isolated lectins or enzymes with lectin domains are disclosed.

Abstract: An automatics system 100 that uses one to three grids 20cm of overhead cameras 20c to first video an event area 2. Overall bandwidth is greatly reduced by intelligent hubs 26 that extract foreground blocks 10m based upon initial and continuously updated background images 2r. The hubs also analyze current images 10c to constantly locate, classify and track in 3D the limited number of expected foreground objects 10. As objects 10 of interest are tracked, the system automatically directs ptz perspective view cameras 40c to follow the activities. These asynchronous cameras 40c limit their images to defined repeatable pt angles and zoom depths. Pre-captured venue backgrounds 2r at each repeatable ptz setting facilitate perspective foreground extraction. The moving background, such as spectators 13, is removed with various techniques including stereoscopic side cameras 40c-b and 40c-c flanking each perspective camera 40c.

Abstract: A power supply including an inverter receiving a DC input signal from a DC input source (11). The inverter is implemented as a single-ended inverter. Each inverter is driven by a signal source (13A, 13B), which outputs an AC signal. The output from each inverter is input to a first stage harmonic filter. The power supply includes an output circuit that includes a rectifier (D1) arranged about a point so that if the inverter attempts to drive the point beyond a predetermined voltage, the rectifier conducts in order to return at least one of power and current to the DC input source. The output from the first harmonic filter (L1A, C1; L1B, C1) is output to a second harmonic filter (L2, C2) and is then output from the power supply.

Abstract: Method and apparatus are provided for buffering a data signal to a low voltage logic device. A circuit for buffering the data signal to the low voltage logic device includes an output buffer (12) having first and second inputs and an output and at least one N-type isolation transistor (13, 19) having a source coupled to one or both of the second input and the output. The first input receives the data signal, the second input receives a supply potential, and the output couples to the low voltage logic device. The isolation transistor has a drain for receiving a first potential and is configured to supply a a second potential to the output buffer when the gate receives a bias potential. The second potential based on the first potential. The bias potential is greater than the supply potential.

Abstract: This invention relates to RV stand offs or breaks and in particular, but not exclusively not to stand offs which are suitable for use in plasma generating apparatus. A stand off 14 includes a resistive or insulating cylinder 15, through which gas feeds are drilled and a close coupled helically wound combination, generally indicated at 16. This combination 16 comprises an RF conductive tube 17 in the form of a braided cable; a thermocouple 18 having twisted pair supply cables 19 and a heater element 20 in the form of a cable loop or pair.

Abstract: A gas-powered resuscitator is operable either in a manual mode or an automatic mode. The resuscitator includes an oscillatory timing valve (14) having an outlet (23) connected to a bi-stable valve (25), the operation of which is piloted by a manual valve (16). The outlet (26) of the bi-stable valve (25) connects to the outlet (2) of the resuscitator via a rotatable control (32) and a patient valve (41). The outlet (26) of the bi-stable valve (25) also connects to the control inlet (34) of the timing valve (14). The manual valve (16) has a button (62) that can be pushed down manually in the manual mode or can be held down in the automatic mode by rotating a locking ring (262). The maximum duration of cycles is limited by operation of the timing valve (14), whether the resuscitator is operated manually or automatically.

Abstract: A gas-powered resuscitator includes an oscillatory timing valve (14) having an outlet (23) connected via a manually-operable valve assembly (16, 25) to a patient valve (41). The timing valve (14) has a sealing rod (145) movable along a bore (141) between a forward position in which it blocks flow through the valve and a rear position in which it enables flow through the valve. The rear end (151) of the rod (145) is mounted in a piston (146) and is urged rearwardly to an open position by a spring (148). A flexible diaphragm (149) is joined around its edge (158) to the housing (140) of the valve, separating the rear end (159) of the valve from the forward end (160) and extending over the rear end (146) of the piston (146). The control inlet (34) of the valve (14) opens into its rear part (159) so that gas pressure applied to the inlet acts on one side of the diaphragm (149) to urge the piston (146) and rod (145) forwardly to the closed position against the action of the spring (148).

Abstract: A novel gene defining a novel enzyme UDP-Galactose: ?-D-Galactose-R 4-?-D-galactosyltransferase, termed ?4Gal-T1, with unique enzymatic properties is disclosed. The invention discloses isolated DNA molecules and DNA constructs encoding ?4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting ?4Gal-T1 activity, as well as cloning and expression vectors including such DNA, cells transfected with vectors, and recombinant methods for providing ?4Gal-T1. The enzyme ?4Gal-T1 and ?4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining ?1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active ?4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active ?4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids.

Abstract: A power supply including an inverter receiving a DC input signal from a DC input source (11). The inverter is implemented as a single-ended inverter. Each inverter is driven by a signal source (13A, 13B), which outputs an AC signal. The output from each inverter is input to a first stage harmonic filter. The power supply includes an output circuit that includes a rectifier (D1) arranged about a point so that if the inverter attempts to drive the point beyond a predetermined voltage, the rectifier conducts in order to return at least one of power and current to the DC input source. The output from the first harmonic filter (L1A, C1; L1B, C1) is output to a second harmonic filter (L2, C2) and is then output from the power supply.

Abstract: Methods of identifying activators and inhibitors of voltage-gated ion channels are provided in which the methods employ cells transfected with a voltage-gated ion channel of interest and a corollary channel to control the membrane potential of the cells by changing extracellular ion concentration. This allows for more convenient, more precise experimental manipulation of these transitions, and, coupled with efficient methods of detecting the result of ion flux through the channels, provides methods that are especially suitable for high throughput screening.

Abstract: This invention relates to RV stand offs or breaks and in particular, but not exclusively not to stand offs which are suitable for use in plasma generating apparatus. A stand off 14 includes a resistive or insulating cylinder 15, through which gas feeds are drilled and a close coupled helically wound combination, generally indicated at 16. This combination 16 comprises an RF conductive tube 17 in the form of a braided cable; a thermocouple 18 having twisted pair supply cables 19 and a heater element 20 in the form of a cable loop or pair.

Abstract: Methods of identifying activators and inhibitors of voltage-gated ion channels are provided in which the methods employ electrical field stimulation of the cells in order to manipulate the open/close state transition of the voltage-gated ion channels. This allows for more convenient, more precise experimental manipulation of these transitions, and, coupled with efficient methods of detecting the result of ion flux through the channels, provides methods that are especially suitable for high throughput screening.

Abstract: An apparatus and method for manipulating scanned documents using computer aided design (CAD) commands includes a link program which receives the CAD commands and displays all or selected portions of a raster-based image, which is created by scanning a hard copy document. The link program will display the portion of the raster image which fits within a predetermined CAD window and will scale the raster image up or down as necessary to fit within the display window. The raster image is displayed on a computer screen and provides a useful reference for an operator to create a CAD image which is a duplicate of the raster image by tracing directly over the raster image or, alternatively, to implement desired changes in the raster image using CAD commands. An edit program merges the CAD image and the original raster image to create a new, updated raster image, which includes all CAD-generated changes made by the operator. The updated raster image can be reproduced in hard copy form by a raster plotter.

Abstract: A new and distinct cultivar of Coreopsis plant named ‘Coreopram’, characterized by its upright, outwardly spreading and mounded plant habit; vigorous growth habit; freely branching habit; freely flowering habit; and single inflorescences with dark red and yellow bi-colored ray florets.