Abstract: Systems and methods for inline and automatic dilution of chemicals of interest for speciation and subsequent analysis by ICP spectrometry are described. A system embodiment includes a first valve to receive a sample into a holding loop; a plurality of syringe pumps coupled to the first valve to deliver an inline diluted sample from the first valve; and a second valve coupled to the first valve to receive the inline diluted sample from the first valve into a sample holding loop coupled to the second valve, the second valve configured to couple to at least one of an eluent source or a carrier fluid source to receive at least one of an eluent fluid or a carrier fluid to transfer the inline diluted sample from the sample holding loop to a speciation column to separate one or more species from the inline diluted sample.

Abstract: A method for diluting a sample includes drawing an aliquot of the sample past a mixing junction. The method also includes supplying the sample to the mixing junction and supplying a second fluid to the mixing junction. The second fluid mixes with the sample at the mixing junction to generate a diluted sample. The method further includes supplying the diluted sample from the mixing junction.

Abstract: A system includes a first aerosolization device (e.g., a nebulizer and a spray chamber/desolvation device in fluid communication with the nebulizer) configured to furnish a first aerosol, and a second aerosolization device (e.g., a second nebulizer and a second spray chamber/desolvation device in fluid communication with the second nebulizer) configured to furnish a second aerosol. The first aerosolization device is balanced with the second aerosolization device. The system also includes an output coupled with the first aerosolization device and the second aerosolization device. The output is configured to supply at least one of the first aerosol or the second aerosol (e.g., to a torch). The system further includes a selection device coupling the first aerosolization device and the second aerosolization device to the output. The selection device is configured to selectively provide at least one of the first aerosol or the second aerosol to the output.

Abstract: A method for internal standardization of cool plasma ICP-MS using one or more enriched stable isotopes includes introducing an enriched stable isotope of a chemical species to a sample containing a non-enriched isotope of the chemical species to form a sample and standard mixture. In implementations, the enriched stable isotope is introduced via an inline syringe addition to a flow of a sample solution containing a non-enriched isotope of the chemical species to be analyzed. The method also includes introducing the sample and standard mixture to an ICP-MS under cool plasma conditions. The method also includes determining an ionization amount of the enriched stable isotope by the ICP-MS. The method further includes correlating an ionization amount of the non-enriched isotope based on the determined ionization amount of the enriched stable isotope.

Abstract: A method including obtaining metadata specifying screens based on a business logic of the matter management workflow, generating, based on the metadata, the screens each including screen objects, where the matter management workflow includes a sequence of the screen objects specified by the metadata based on the business logic, receiving a matter management user input via the screen objects to navigate the matter management workflow, receiving a screen tester user input specifying a test instruction including a first specification based on the screen objects, generating, using the first specification and without further user intervention, a test sequence including a second specification based on the metadata, applying the test sequence to the screen objects to generate a first result depicting an evaluation of navigating the matter management workflow according to the business logic, and generating an alert in response to the first result indicating a violation to the business logic.

Abstract: The invention provides a method of preventing the formation of inhibitory antibodies in a mammal undergoing gene therapy. The method comprises administering to the mammal an immunosuppressive agent in conjunction with the gene therapy.

Abstract: A method including obtaining metadata specifying screens based on a business logic of the matter management workflow, generating, based on the metadata, the screens each including screen objects, where the matter management workflow includes a sequence of the screen objects specified by the metadata based on the business logic, receiving a matter management user input via the screen objects to navigate the matter management workflow, receiving a screen tester user input specifying a test instruction including a first specification based on the screen objects, generating, using the first specification and without further user intervention, a test sequence including a second specification based on the metadata, applying the test sequence to the screen objects to generate a first result depicting an evaluation of navigating the matter management workflow according to the business logic, and generating an alert in response to the first result indicating a violation to the business logic.

Abstract: Specific binding members comprising human antibody antigen binding domains specific for human transforming growth factor beta (TGF?) bind specifically isoforms TGF?2 and TGF?1 or both, preferentially compared with TGF?3. Specific binding members may be isolated and utilized in the treatment of disease, particularly fibrotic disease and also immune/inflammatory diseases. Therapeutic utility is demonstrated using in vitro and in vivo models. Full sequence and binding information is provided, including epitope sequence information for particularly advantageous specific binding member which binds the active form of TGF?2, neutralizing its activity, but does not bind the latent member.

Abstract: The invention may be summarized as follows. The present invention provides insecticides comprised of naturally-occurring compounds, such as, for example, PA1b-related peptides and terpenoid saponins. The present invention also provides for identification and characterization of a synergistic insecticidal effect between peptides and saponins extracted from plants. Furthermore, simplified extraction procedures are provided that avoid the use of a chloroform defatting step or a column chromatography step.

Abstract: This invention describes a surface treatment composition, preferably for personal use, comprising at least one surfactant and at least two different organic acids and/or salts or organic acids, and wherein the total concentration of the organic acids and/or salts in the composition is at least 0.5% (w/v). The invention further extends to a method of treating a surface, preferably hair or skin, to remove dirt and to remove or inhibit microbial growth.

Abstract: A process for the production of a protein by cell culture, where the cells that produce the protein are cultured in the presence of a chemical agent that enhances the production of the protein.

Abstract: An animal cell culture medium is described which contains 2-hydroxy-2,4,6-cycloheptatrien-1-one or a derivative thereof to support the growth of animal cells, particularly in agitated cell culture at low iron concentrations.

Abstract: A process for the production of a protein by cell culture, where the cells that produce the protein are cultured in the presence of a chemical agent that enhances the production of the protein.

Abstract: Methods for obtaining a protein by culture of hybridoma cells, wherein said protein is an immunoglobulin, are disclosed. The methods involve culturing animal hybridoma cells in continuous presence of an alkanoic acid or salt thereof, which enhances protein production, wherein said alkanoic acid or salt thereof is present at 2 concentration range of 0.1 mM to 200 mM.

Abstract: Methods for enhancing the production of tissue plasminogen activator (tPA) in cell culture are disclosed. The methods involve culturing tPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances tPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.5 mM and 2.5 mM.

Abstract: Methods for enhancing the production of tissue plasminogen activator (tPA) in cell culture are disclosed. The methods involve culturing tPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances tPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.1 mM and 10 mM.

Abstract: An insecticidal dust composition comprising an effective amount of diatomaceous earth (DE) in combination with an effective amount of silica selected from the group consisting of precipitated silica and aerogel silica, the DE and silica mixed in different proportions by weight depending on the type of DE, and may preferably be about 95% to 65% DE to about 5% to 35% silica.

Abstract: Methods for enhancing the production of tissue plasminogen activator (TPA) in cell culture are disclosed. The methods involve culturing TPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances TPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.5 mM and 2.5 mM.

Abstract: An electric trouble finding apparatus comprises means to test either hot or cold circuits with a single unit. The apparatus comprises a first pair of binding posts which serve as the coupling means to a hot output and a second pair of binding posts which may serve simultaneously as connecting means to a cold or inert circuit. The unit includes a four pole double throw switch having a single lever control and a plurality of twelve terminals in the switch arrangement. A first pair of oppositely positioned switch terminals are connected to an AC power source while a second pair of opposite terminals in the switch are connected to respective hot binding posts. One of the cold binding posts is connected through a lamp to a terminal in the switch while the opposite terminal is coupled to the other cold binding post. A pair of terminals in the switch are interconnected while another pair of terminals in the switch are coupled to the hot outlet from the power supply.