Patents by Inventor Paul S. Weiss
Paul S. Weiss has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230416608Abstract: The present invention provides devices comprising at least one liquid crystal layer and methods of use thereof. The present invention also provides methods for generating said devices.Type: ApplicationFiled: December 3, 2021Publication date: December 28, 2023Inventors: Amir Nasajpour, Paul S. Weiss
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Patent number: 11684922Abstract: A microfluidic-based device and system is disclosed for the high-throughput intracellular delivery of biomolecular cargo to cells (eukaryotic or prokaryotic) or enveloped viruses. Cargo integration occurs due to transient membrane permeabilization by exposure to bulk acoustic waves (BAWs) transduced from surface acoustic waves (SAWs) generated by a rapidly oscillating piezoelectric substrate. In this approach, temporary pores are established across the cellular membrane as cells are partially deformed and squeezed or subject to shearing forces as they travel through the vibrational modes created within the microfludic channel(s) of the device.Type: GrantFiled: February 13, 2018Date of Patent: June 27, 2023Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Paul S. Weiss, Steven J. Jonas, Dan Wilkinson, Adam Z. Stieg, Jason Belling
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Publication number: 20210346889Abstract: A microfluidic device is disclosed that is used to process cells for the intracellular delivery of molecules or other cargo. The device includes one or more microchannels disposed in a substrate or chip and is fluidically coupled to an inlet configured to receive a solution containing the cells and the molecules or other cargo to be delivered intracellularly to the cells. Each of the one or more microchannels has one or more constriction regions formed therein, wherein the inner surface(s) of the microchannels and the one or more constriction regions have a lipid bilayer disposed thereon. In some embodiments, multiple microfluidic devices operating in parallel are used to process large numbers of cells. The device and method have particularly applicability to delivering gene-editing molecules intracellularly to cells.Type: ApplicationFiled: August 21, 2019Publication date: November 11, 2021Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, NANYANG TECHNOLOGICAL UNIVERSITYInventors: Jason N. Belling, Steven J. Jonas, Joshua A. A. Jackman, Nam-Joon Cho, Paul S. Weiss
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Publication number: 20210308671Abstract: Systems and methods are disclosed that utilize metal nanostructures that are synthesized in situ along the internal surfaces of a microfluidic device. The nanostructures are formed by initial deposition of metallic seeds followed by flowing growth and reducing agent solutions into the capillaries/microfluidic channels to grow the nanostars. The nanostructures may optionally be functionalized with a capture ligand. The capture ligand may be used to selectively bind to certain cells (e.g., circulating tumor cells). The cells may be removed by a beam of light (e.g., laser beam) that induces localized heating at the surface location(s) containing the nanostructures. The plasmonic nature of the nanostructures can be used to heat the nanostructure(s) locally for the selective removal of one or certain cells. The nanostructures may be used to acquire Raman spectra of molecules or other small objects that are bound thereto for identification and quantification.Type: ApplicationFiled: August 16, 2019Publication date: October 7, 2021Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Leonardo Scarabelli, Gail Vinnacombe, Liv Heidenreich, Naihao Chiang, Yao Gong, Paul S. Weiss, Steven J. Jonas
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Publication number: 20210223240Abstract: Devices for detecting at least one target molecule in a sample are provided. The devices comprise a field-effect transistor and an aptamer attached to the field-effect transistor. The aptamer comprises a capture region and a stem region, wherein the target molecule can selectively bind to the capture region of the aptamer. The stem region can change a conformation of the aptamer when the capture region binds to the target molecule. Techniques for detecting a target molecule using such devices are also provided.Type: ApplicationFiled: February 12, 2021Publication date: July 22, 2021Applicants: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Milan STOJANOVIC, Anne Milasincic ANDREWS, Kyungae YANG, Paul S. WEISS, Nako NAKATSUKA
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Patent number: 11037794Abstract: A robust and general fabrication/manufacturing method is described herein for the fabrication of periodic three-dimensional (3D) hierarchical nanostructures in a highly scalable and tunable manner. This nanofabrication technique exploits the selected and repeated etching of spherical particles that serve as resist material and that can be shaped in parallel for each processing step. The method enables the fabrication of periodic, vertically aligned nanotubes at the wafer scale with nanometer-scale control in three dimensions including outer/inner diameters, heights/hole-depths, and pitches. The method was utilized to construct 3D periodic hierarchical hybrid silicon and hybrid nanostructures such as multi-level solid/hollow nanotowers where the height and diameter of each level of each structure can be configured precisely as well as 3D concentric plasmonic supported metal nanodisk/nanorings with tunable optical properties on a variety of substrates.Type: GrantFiled: September 25, 2019Date of Patent: June 15, 2021Assignee: The Regents of the University of CaliforniaInventors: Xiaobin Xu, Qing Yang, Natcha Wattanatorn, Chuanzhen Zhao, Logan A. Stewart, Steven J. Jonas, Paul S. Weiss
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Publication number: 20210140917Abstract: A method of detecting and/or discriminating mismatched or complementary nucleic acids using a field-effect transistor (FET). The FET comprises source and drain electrodes formed on substrate and separated by a channel that includes a thin semiconducting film. One or more nucleic acid molecules are immobilized to thin semiconducting film. The FET includes a gate electrode in contact with solution containing the sample (or located on the surface of the device). Samples possibly containing target nucleic acid are exposed to the FET of the biosensor device and current response is measured after samples containing target nucleic acid have been exposed to the FET of the biosensor device. Measured current response in the FET is used to detect and/or discriminate whether target nucleic acid is present as well as complementary or mismatched. Measured current response may also be used to differentiate among different mismatches.Type: ApplicationFiled: April 11, 2019Publication date: May 13, 2021Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Paul S. Weiss, Anne M. Andrews, Kevin M. Cheung
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Patent number: 10907124Abstract: The present invention relates to biomimetic membrane compositions and methods for making and using them.Type: GrantFiled: January 31, 2019Date of Patent: February 2, 2021Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Alireza Moshaverinia, Mohammad Mahdi Hasani-Sadrabadi, Paul S. Weiss, Tara L. Aghaloo
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Publication number: 20200397948Abstract: A dental hydrogel composition comprising: (a) polysaccharide; (b) polydopamine conjugated to the polysaccharide, wherein between 5 and 35 percent of polysaccharide sugar moieties are conjugated to polydopamine; (c) RGD peptide coupled to the polysaccharide-poly-dopamine conjugate; and (d) moieties that are crosslinkable upon exposure to light coupled to the polysaccharide; wherein components (a)-(d) are disposed in the composition such that the hydrogel composition: exhibits an adhesive strength of at least 10 kPa upon cross linking of crosslinkable moieties; and exhibits an elasticity between 5 kPa and 100 kPa upon cross linking of crosslinkable moieties.Type: ApplicationFiled: March 11, 2019Publication date: December 24, 2020Applicant: The Regents of the University of CaliforniaInventors: Alireza MOSHAVERINIA, Mohammad Mahdi HASANI-SADRABADI, Tara L. AGHALOO, Paul S. WEISS
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Publication number: 20200347409Abstract: A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs.Type: ApplicationFiled: January 29, 2019Publication date: November 5, 2020Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Paul S. Weiss, Hsian-Rong Tseng, Xiaobin Xu, Natcha Wattanatorn, Qing Yang, Steven J. Jonas
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Patent number: 10620107Abstract: Systems and methods of fabricating and functionality patterned nanowire probes that are stable under fluid reservoir conditions and have imageable contrast are provided. Optical imaging and deconstruction methods and systems are also provided that are capable of determining the distribution of nanowires of a particular pattern to determine the mixing between or leakage from fluid reservoirs.Type: GrantFiled: May 5, 2015Date of Patent: April 14, 2020Assignee: The Regents of the University of CaliforniaInventors: Paul S. Weiss, Anne M. Andrews, Andrea L. Bertozzi, Stanley J. Osher
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Publication number: 20200098577Abstract: A robust and general fabrication/manufacturing method is described herein for the fabrication of periodic three-dimensional (3D) hierarchical nanostructures in a highly scalable and tunable manner. This nanofabrication technique exploits the selected and repeated etching of spherical particles that serve as resist material and that can be shaped in parallel for each processing step. The method enables the fabrication of periodic, vertically aligned nanotubes at the wafer scale with nanometer-scale control in three dimensions including outer/inner diameters, heights/hole-depths, and pitches. The method was utilized to construct 3D periodic hierarchical hybrid silicon and hybrid nanostructures such as multi-level solid/hollow nanotowers where the height and diameter of each level of each structure can be configured precisely as well as 3D concentric plasmonic supported metal nanodisk/nanorings with tunable optical properties on a variety of substrates.Type: ApplicationFiled: September 25, 2019Publication date: March 26, 2020Applicant: The Regents of the University of CaliforniaInventors: Xiaobin Xu, Qing Yang, Natcha Wattanatorn, Chuanzhen Zhao, Logan A. Stewart, Steven J. Jonas, Paul S. Weiss
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Publication number: 20190381507Abstract: A microfluidic-based device and system is disclosed for the high-throughput intracellular delivery of biomolecular cargo to cells (eukaryotic or prokaryotic) or enveloped viruses. Cargo integration occurs due to transient membrane permeabilization by exposure to bulk acoustic waves (BAWs) transduced from surface acoustic waves (SAWs) generated by a rapidly oscillating piezoelectric substrate. In this approach, temporary pores are established across the cellular membrane as cells are partially deformed and squeezed or subject to shearing forces as they travel through the vibrational modes created within the microfludic channel(s) of the device.Type: ApplicationFiled: February 13, 2018Publication date: December 19, 2019Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Paul S. Weiss, Steven J. Jonas, Dan Wilkinson, Adam Z. Stieg, Jason Belling
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Publication number: 20190276787Abstract: The present invention relates to biomimetic membrane compositions and methods for making and using them.Type: ApplicationFiled: January 31, 2019Publication date: September 12, 2019Applicant: The Regents of the University of CaliforniaInventors: Alireza MOSHAVERINIA, Mohammad Mahdi HASANI-SADRABADI, Paul S. Weiss, Tara L. Aghaloo
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Publication number: 20190187040Abstract: Systems and methods of fabricating and functionalizing patterned nanowire probes that are stable under fluid reservoir conditions and have imageable patterns are provided. Additional embodiments possess functionalized molecular groups to collect content data of desired chemical species from a fluid reservoir in order to detect even highly dilute quantities of the desire chemical species in the reservoir. Optical imaging and deconstruction methods and systems are also provided that are capable of determining the distribution of nanowires of a particular pattern to determine the mixing between or leakage from fluid reservoirs.Type: ApplicationFiled: February 25, 2019Publication date: June 20, 2019Applicant: The Regents of the University of CaliforniaInventors: Paul S. Weiss, Anne M. Andrews, Andrea L. Bertozzi, Stanley J. Osher
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Publication number: 20190177677Abstract: A microfluidic device for processing cells for the intracellular delivery of molecules or other cargo includes a plurality of microchannels disposed in a substrate or chip and fluidically coupled to an inlet configured to receive a solution containing the cells and the molecules or other cargo to be delivered intracellularly to the cells. Each of the plurality of microchannels has one or more constriction regions therein, wherein the constriction regions comprise an omniphobic, superhydrophilic, or superhydrophobic surface. In some embodiments, multiple microfluidic devices operating in parallel are used to process large numbers of cells. The device and method has particularly applicability to delivering gene-editing molecules intracellularly to cells.Type: ApplicationFiled: August 19, 2017Publication date: June 13, 2019Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, PRESIDENT AND FELLOWS OF HARVARD COLLEGE, BRIGHAM AND WOMEN'S HOSPITALInventors: Steven J. Jonas, Paul S. Weiss, Xu Hou, Joanna Aizenberg, Alireza Khademhosseini
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Patent number: 9630161Abstract: Methods and assemblies for the construction of liquid-phase alloy nanoparticles are presented. Particle formation is directed by molecular self-assembly and assisted by sonication. In some embodiments, eutectic gallium-indium (EGaIn) nanoparticles are formed. In these embodiments, the bulk liquid alloy is ultrasonically dispersed, fast thiolate self-assembly at the EGaIn interface protects the material against oxidation. The assembly shell has been designed to include intermolecular hydrogen bonds, which induce surface strain, assisting in cleavage of the alloy particles to the nanoscale. X-ray diffraction and TEM analyses reveal that the nanoscale particles are in an amorphous or liquid phase, with no observed faceting.Type: GrantFiled: March 13, 2013Date of Patent: April 25, 2017Assignee: The Regents of the University of CaliforniaInventors: James Nathan Hohman, Paul S. Weiss
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Publication number: 20170052104Abstract: Systems and methods of fabricating and functionality patterned nanowire probes that are stable under fluid reservoir conditions and have imageable contrast are provided. Optical imaging and deconstruction methods and systems are also provided that are capable of determining the distribution of nanowires of a particular pattern to determine the mixing between or leakage from fluid reservoirs.Type: ApplicationFiled: May 5, 2015Publication date: February 23, 2017Applicant: The Regents of the University of CaliforniaInventors: Paul S. Weiss, Anne M. Andrews, Andrea L. Bertozzi, Stanley J. Osher
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Publication number: 20150328616Abstract: Surface mediated polymer synthesizing methods and related systems and materials are described where monomers are attached to monomer binding regions on a surface and subsequently form chemical bonds with adjacent monomers on the surface to form linear polymers selected from polynucleotide, polypeptides and polysaccharides.Type: ApplicationFiled: May 13, 2015Publication date: November 19, 2015Inventors: Matthew GETHERS, William A. GODDARD, III, Paul S WEISS, John RANDALL
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Publication number: 20130244037Abstract: Methods and assemblies for the construction of liquid-phase alloy nanoparticles are presented. Particle formation is directed by molecular self-assembly and assisted by sonication. In some embodiments, eutectic gallium-indium (EGaIn) nanoparticles are formed. In these embodiments, the bulk liquid alloy is ultrasonically dispersed, fast thiolate self-assembly at the EGaIn interface protects the material against oxidation. The assembly shell has been designed to include intermolecular hydrogen bonds, which induce surface strain, assisting in cleavage of the alloy particles to the nanoscale. X-ray diffraction and TEM analyses reveal that the nanoscale particles are in an amorphous or liquid phase, with no observed faceting.Type: ApplicationFiled: March 13, 2013Publication date: September 19, 2013Applicant: The Regents of the University of CaliforniaInventors: James Nathan Hohman, Paul S. Weiss