Abstract: The invention provides a method for rapid cloning of T-cell receptors (TCRs) (e.g., paired ?? and ?? TCR chains) and B-cell receptors (BCRs) (e.g., paired IgH or IgK or Ig?) from single cells by CDR3 substitution using single cell PCR products and Gibson Assembly techniques and a pre-generated TCR (or BCR) library in an expression vector.

Abstract: Embodiments of the invention provide an electric heat trace (EHT) control system for use with a piping system having pipes and capable of transporting a fluid. The EHT control system includes an EHT circuit for heating the piping system, a temperature sensor outputting a temperature value, a flow status sensor outputting a flow status value, and an EHT management system. The EHT management system can include a controller connected to the EHT circuit in order to selectively energize the EHT circuit. The controller can be connected to the temperature sensor in order to receive the temperature value and the flow status sensor in order to receive the flow status value. The controller can be configured to execute an EHT management program including the steps of receiving the temperature value, receiving the flow status value, and utilizing the temperature value and/or the flow status value to determine whether or not to energize the EHT circuit.

Abstract: A vector harboring nucleic acids encoding a recombinant T cell receptor having antigenic specificity for a DnaJ heat shock protein family member B1-protein kinase cAMP-activated catalytic subunit alpha (DNAJB1-PRKACA) fusion protein is provided, as are host cells and a method for treating fibrolamellar hepatocellular carcinoma with the vector.

Abstract: Embodiments of a recombinant Respiratory Syncytial Virus (RSV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the RSV F ectodomain trimer and methods of producing the RSV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the recombinant RSV F ectodomain trimer to the subject.

Abstract: A brake assembly including a caliper that has a first guide bore, a brake carrier arranged to receive a brake pad, a first guide pin secured to the brake carrier, and a first lock arrangement. The first lock arrangement is operable to selectively inhibit relative sliding between the caliper and the brake carrier.

Abstract: Embodiments of a recombinant human Parainfluenza Virus (hPIV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the hPIV F ectodomain trimer and methods of producing the hPIV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or inhibiting a hPIV infection in a subject by administering a effective amount of the recombinant hPIV F ectodomain trimer to the subject.

Abstract: Platforms, platform systems, and safety grates and related methods used to facilitate the installation of plant-based remediation systems are provided therein. A platform system can include a platform having a frame supporting a flooring structure that has a surface on which a worker can stand. The platform and flooring structure can have central aperture therein. The platform system can also include a sleeve frame positioned proximal to the central aperture. The sleeve frame can have sleeve clamps securable thereto for holding a sleeve ring round at least a portion of the central aperture and to hold a liner sleeve to the sleeve ring. Further, the platform system can also include a safety grate attachable to the platform. The safety grate can comprise a grid of crossbars with folding funnel guideboards secured to the grid of crossbars for funneling soil when backfilling the liner sleeve once the liner sleeve is placed in an excavated hole.

Abstract: A disc brake assembly that includes first and second brake pads for contacting a rotor, a brake carrier for receiving at least the first brake pad, a brake caliper arranged to slide with respect to the brake carrier, and an actuating arrangement. The actuating arrangement is configured to selectively move the brake caliper and is electrically operated.

Abstract: The invention provides a method for rapid cloning of T-cell receptors (TCRs) (e.g., paired ?? and ?? TCR chains) and B-cell receptors (BCRs) (e.g. paired IgH or IgK or Ig?) from single cells by CDR3 substitution using single cell PCR products and Gibson Assembly techniques and a pre-generated TCR (or BCR) library in an expression vector.

Abstract: Embodiments of a recombinant Respiratory Syncytial Virus (RSV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the RSV F ectodomain trimer and methods of producing the RSV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the recombinant RSV F ectodomain trimer to the subject.

Abstract: Disclosed are methods for identifying or determining whether a subject exhibits a CD4+ memory T cell response to SARS-CoV-2 infection or vaccination, assessing the efficacy of a SARS-CoV-2 vaccine, and developing personalized SARS-CoV-2 treatment plans by detecting the presence and/or quantity of a particular T cell receptor a chain that recognizes a specific Spike protein epitope.

Abstract: A kit and method for analyzing nucleic acid molecules encoding T cell receptor (TCR) chains from individual T cells are disclosed. In particular, a method for analyzing individual T cells using high-throughput multiplex amplification and deep sequencing of nucleic acids encoding TCRs is provided.

Abstract: Systems and methods for distributed ledger-based settlement are disclosed. In one embodiment, a method may include: receiving settlement instructions from a first party providing a commodity and a second party providing cash; matching the settlement instructions; affirming the matched settlement instructions with the parties; a smart contact sending the affirmed settlement instructions to a commodity clearing bank; the smart contract receiving confirmation of a blockage of the commodity from the commodity clearing bank; the smart contact sending the affirmed settlement instructions to a cash clearing bank; the smart contract receiving confirmation of a blockage of the cash from the cash clearing bank; the smart contract executing the exchange of tokens for the commodity for tokens for the cash; and the smart contract writing the tokens for the cash to a wallet for the first party, and writing tokens for the commodity to a wallet for the second party.

Abstract: A disc brake, an axle assembly having a disc brake, and a method of assembly. The disc brake may include a first friction element and a second friction element. The first and second friction elements may have an effective contact area with a brake rotor of at least 10,000 mm2.

Abstract: A fairing assembly that includes a fairing body and a storage compartment attached to the fairing body. The storage compartment includes a frame housing that serves as a load-bearing structural member of the fairing body. The frame housing includes an upper frame housing member and a lower frame housing member that collectively define an interior space that receives a retractable drawer mounted therein. The retractable drawer is selectively moveable in a longitudinal direction relative to the frame housing between a closed position and an open position.

Abstract: Embodiments of a recombinant Respiratory Syncytial Virus (RSV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the RSV F ectodomain trimer and methods of producing the RSV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the recombinant RSV F ectodomain trimer to the subject.

Abstract: A produced water evaporation system for spray evaporating water comprising a geothermal heat retainment system and an evaporation system is disclosed. The geothermal heat retainment system comprises a first feed inlet, an optional solar collection system or a heat exchanger, and a first discharge outlet.

Abstract: Embodiments of a recombinant human Parainfluenza Virus (hPIV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the hPIV F ectodomain trimer and methods of producing the hPIV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or inhibiting a hPIV infection in a subject by administering a effective amount of the recombinant hPIV F ectodomain trimer to the subject.

Abstract: Embodiments of a recombinant Respiratory Syncytial Virus (RSV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the RSV F ectodomain trimer and methods of producing the RSV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the recombinant RSV F ectodomain trimer to the subject.