Abstract: Provided herein are libraries containing polynucleotides, where one of the polynucleotides encodes an antibody light chain with specific hypervariable regions HVR-L1, HVR-L2, and HVR-L3. Further provided herein are libraries containing polynucleotides encoding a plurality of unique antibodies, wherein each antibody comprises a heavy chain variable region and a light chain variable region. Also provided are antibodies, polypeptide libraries, vector libraries, cells, non-human animals, antibody light chains, methods of making an antibody library, kits, and methods of generating a bispecific antibody related thereto.

Abstract: A poor foundation reinforcement system and a reinforcement method based on underground concealed arch structures are provided. The system and the method belong to the technical field of poor foundation treatment. The reinforcement system includes multiple columns of pile foundations arranged along a bridge direction. Multiple arch sheets are set in parallel between each two adjacent columns of pile foundations. Each arch sheet includes multiple high-pressure rotary jet piles arranged along the bridge. Each two adjacent arch sheets of the multiple arch sheets are connected by a micro-bending slab disposed on the two adjacent arch sheets, the multiple arch sheets are connected by the micro-bending slabs to together form an arch ring, and a construction joint is disposed between each two adjacent micro-bending slabs of the micro-bending slabs.

Abstract: A patterning method of a film is disclosed. The method including: providing a film including a first surface; forming n etching barrier layers on the first surface of the film, and n is an integer larger than or equal to 2; and performing n etching processes on the film to form a recessed structure on the first surface using the n etching barrier layers as masks, the recessed structure includes n bottom surfaces respectively having different depths. Two adjacent etching processes of the n etching processes include a previous etching process and a subsequent etching process, and after the previous etching process is completed, a part of the n etching barrier layers is removed to form a mask for the subsequent etching process; a material of the part of the n etching barrier layers which is removed is different from a material of the mask of the subsequent etching process.

Abstract: The present application provides compositions and methods for treating cancers in a subject using an anti-CD137 antibody and an agent that induces expression of CD137 on an immune cell and/or induces expression of CD137L on a cancer cell of the subject. In some embodiments, the agent is a cytokine such as IL-2. In some embodiments, the agent is a histone deacetylase (HDAC) inhibitor. In some embodiments, the agent is a DNA-damaging agent.

Abstract: A gene sequencing substrate and a method for manufacturing the same, and a gene sequencing device are provided. It belongs to the technical field of gene sequencing, and can solve the problem of high cost of the high-throughput sequencing chip in the prior art. The gene sequencing substrate of the present disclosure comprises a plastic material with concave structures as base substrate, and the concave structures serve as reaction cells. Since the base substrate has plasticity, the concave structures can be formed by a simple process to reduce the cost of the gene sequencing substrate. Meanwhile, a first protective layer may be provided on the inner wall of the concave structures for preventing the inner wall of the concave structures from being corroded by the reaction liquid.

Abstract: A phase-shift unit includes: a first substrate and a second substrate provided opposite to each other; a medium layer provided between the first substrate and the second substrate; a microstrip line disposed at a side of the second substrate facing towards the first substrate; and a grounding layer provided at a side of the first substrate facing towards the second substrate and formed with a via hole; wherein a projection of the via hole onto the second substrate and a projection of the microstrip line onto the second substrate have an overlapped area therebetween; and wherein the via hole is configured to feed a phase-shifted microwave signal out of the phase-shift unit, or feed a microwave signal into the phase-shift unit such that the microwave signal is phase-shifted.

Abstract: Disclosed are an array substrate and a preparation method thereof, and a digital microfluidic chip. The preparation method includes: forming a plurality of photoelectric detection devices on a silicon-based substrate; transferring the photoelectric detection devices to a base substrate by adopting a micro transfer printing process; and forming a plurality of transparent driving electrodes on the base substrate, wherein the transparent driving electrodes are insulated from the photoelectric detection devices.

Abstract: A phase-shift unit includes: a first substrate and a second substrate provided opposite to each other; a medium layer provided between the first substrate and the second substrate; a microstrip line disposed at a side of the second substrate facing towards the first substrate; and a grounding layer provided at a side of the first substrate facing towards the second substrate and formed with a via hole; wherein a projection of the via hole onto the second substrate and a projection of the microstrip line onto the second substrate have an overlapped area therebetween; and wherein the via hole is configured to feed a phase-shifted microwave signal out of the phase-shift unit, or feed a microwave signal into the phase-shift unit such that the microwave signal is phase-shifted.

Abstract: A digital microfluidic chip and a digital microfluidic system. The digital microfluidic chip comprises: an upper substrate and a lower substrate arranged opposite to each other; multiple driving circuits and multiple addressing circuits disposed between the lower substrate and the upper substrate; and a control circuit, electrically connected to the driving circuits and the addressing circuits. The control circuit is configured to apply, in a driving stage, a driving voltage to each driving circuit, such that a droplet is controlled to move inside a droplet accommodation space according to a set path, measure, in a detection stage, after a bias voltage is applied to each addressing circuit, a charge loss amount of each addressing circuit, and to determine the position of the droplet according to the charge loss amount. The charge loss amount of each addressing circuit is related to the intensity of received external light.

Abstract: Provided herein are cross-reactive antibodies (or antigen binding fragments thereof) that bind to human CTLA4, activatable antibodies that bind to human CTLA4, nucleic acid molecules encoding the same, pharmaceutical compositions thereof, and methods of their therapeutic use (e.g., for treatment of cancer).

Abstract: The disclosure relates to antibodies against human CSF-1R, methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof.

Abstract: A chip for polymerase chain reaction, a method of operating a chip for polymerase chain reaction, and a reaction device are provided. The chip includes: a sample adding region, a mixing region, a temperature cycling region in a sequential arrangement, and at least one driving unit group. The at least one driving unit group includes a plurality of driving units and is configured to drive a liquid drop to move and sequentially pass through the sample adding region, the mixing region, and the temperature cycling region.

Abstract: A system for simultaneously measuring 3DOF LGEs by a laser and a method therefor, including a measuring unit and a target mirror unit, the measuring unit includes a laser emitting module, a polarizing beam splitter, a fixed reflector, a first photodetector, and an interference length measuring module; the target mirror unit includes a reflector; the laser emitting module generates an emitting light L1, the polarizing beam splitter is used for 1) “beam splitting” comprising splitting the emitting light L1 into a measuring light L11 and a reference light L12, the measuring light L11 is incident on the target mirror unit and is reflected back by the target mirror unit, so as to return to the measuring unit with a 3DOF LGEs signal; and 2) “beam combining” making the measuring light L11 and the reference light L12 superposed with each other at a spatial position, so as to form a combined beam L3; by measuring a position, frequency and phase drifts of the light L3, the 3DOF LGEs of a space object moving linearly al

Abstract: A method and system for simultaneously measuring multiple DOF GEs by a laser. The system comprises a measuring unit and a target mirror unit; the measuring unit comprises a laser emitting module, a polarizing beam splitter, a fixed reflector, a first ?/4 wave plate, a second ?/4 wave plate, a first polarizer, a first photodetector, an interference length measuring module and a 2D angle measuring module. The target mirror unit comprises a beam splitter and a reflector. The laser emitting module generates an emitting light L1. The polarizing beam splitter is used for (1) beam splitting, (2) beam combining, and (3) beam separating. The fixed reflector is used for reflecting backward the reference light L12 propagating only inside the measuring unit to return the reference light L12 to the polarizing beam splitter.

Abstract: The present application provides compositions and methods for treating cancers, including follicular lymphoma, T cell lymphoma and adenoid cystic carcinoma, using an anti-CD137 antibody that specifically binds to an extracellular domain of human CD137. In some embodiment, combination therapies including the anti-CD137 antibody and an immune checkpoint inhibitor, and/or a chemotherapeutic agent are provided. Biomarkers such as total CD137, membrane bound CD137 (mCD137), soluble CD137 (sCD137), CD137 ligand, Ki67, CD8+ effector memory T (Tem) cells, regulatory T (Treg) cells, and natural killer (NK) cell levels for the methods of treatment described herein are also provided.

Abstract: The present disclosure provides a signal conditioner, an antenna device and a manufacturing method. The signal conditioner includes: a microstrip line including a first portion and a second portion; an insulating layer including a first insulating layer covering the first portion; at least one electrode; a liquid crystal layer covering the microstrip line, the insulating layer, and the at least one electrode; and a common electrode line. A first end of the first portion is connected to a first end of the second portion. A second end of the first portion is connected to a second end of the second portion. The at least one electrode includes a first electrode on a side of the first insulating layer facing away from the first portion. The common electrode line is on a side of the liquid crystal layer facing away from the microstrip line.

Abstract: Provided are heterodimeric proteins comprising polypeptides having CH3 domains with engineered residues that form disulfide bonds and/or salt bridges. Also provided are activatable antibodies targeting CD3 and/or HER2. Compositions, methods of manufacture and methods of treatment using the heterodimeric proteins and the activatable antibodies are further provided.

Abstract: A liquid crystal phase shifter, a liquid crystal antenna, a communication apparatus, and a method for operating a liquid crystal phase shifter are provided. The liquid crystal phase shifter includes a microwave transmission structure and a phase adjustment structure opposite to each other, and a liquid crystal layer between the microwave transmission structure and the phase adjustment structure; wherein the phase adjustment structure comprises a plurality of phase adjustment units; the plurality of phase adjustment units are configured to change a dielectric constant of the liquid crystal layer according to a voltage applied to the phase adjustment units and a voltage applied to the microwave transmission structure, so as to adjust a phase of a microwave signal; and phase shift amounts adjusted by at least two of the plurality of phase adjustment units are different from each other.

Abstract: Provided herein are libraries containing polynucleotides, where one of the polynucleotides encodes an antibody light chain with specific hypervariable regions HVR-L1, HVR-L2, and HVR-L3. Further provided herein are libraries containing polynucleotides encoding a plurality of unique antibodies, wherein each antibody comprises a heavy chain variable region and a light chain variable region. Also provided are antibodies, polypeptide libraries, vector libraries, cells, non-human animals, antibody light chains, methods of making an antibody library, kits, and methods of generating a bispecific antibody related thereto.

Abstract: There is provided an influenza virus detection chip and a method for detecting influenza virus therewith. An influenza virus detection chip including: a graphene oxide film; a first pad disposed on one side of the graphene oxide film in a first direction; and a first electrode and a second electrode, connected to both ends of the graphene oxide film in a second direction perpendicular to the first direction, wherein a first monoclonal antibody with a fluorescent label is included in the first pad, and a second monoclonal antibody is included in the graphene oxide film, and wherein the fluorescent label includes a C?C—C?C conjugated double bond.