Abstract: The present invention relates to the field of biotechnology engineering. It provides a method of constructing a recombinant Bacillus subtilis that can produce specific-molecular-weight hyaluronic acids. By integranted expression of hasA from Streptococcus zooepidemicus and overexpression of genes of HA synthetic pathway, tuaD, glmU and glmS, high yield HA production was achieved in the recombinant strain. Additionally, introduction and functional expression of the leech hyaluronidase in the recombinant strain substantially increased the yield of HA to 19.38 g·L?1. Moreover, HAs with a broad range of molecular weights (103 Da to 106 MDa) were efficiently produced by controlling the expression level of hyaluronidase using RBS mutants with different translational strengths. The method of the present invention can be used to produce low molecular weight HAs at large scale in industrial applications.

Abstract: A real-time birefringent imaging spectrometer based on differential structure is provided. A polarization beam splitter to add an imaging branch is set. Meanwhile, the structure alters the conventional optical structure to the differential structure by setting another polarization beam splitter. Taking the difference between these two interferogram obtained by two branches of differential structure as the final interferogram and performing the required post-processing calculations produces the spectrum at each pixel. Common-mode error is intensively restrained due to the differential structure, and 50% of the optical loss is avoided because of casting off the analyzer; a high spatial resolution, high spectral resolution image is acquired by combining the high spatial resolution, colorful image with the low spatial resolution, high spectral resolution image.

Abstract: Provided is a protein coded by a gene related to cell totipotency and a transcriptional activation domain of a mammalian YAP protein or a fusion protein of a segment with a transcriptional control activity, a coding nucleotide sequence, an expression vector and a composition thereof, as well as a method for inducing the pluripotent stem cells by using the fusion protein.

Abstract: Embodiments relate generally to systems and methods for detecting gas using a wide band light source, and at least two narrow band light sensors, wherein each sensor may be operable to detect light at a different range of wavelengths. Additionally, the gas detector device may comprise a color changing indicator operable to react with gas in the air to change color, wherein different gases may react to create different colors, and wherein the sensors detect light reflected by the color changing indicator from the wide band light source. The gas detector device may comprise a processor in communication with the at least two light sensors operable to receive detected reflected wavelength information from the light sensors, and determine the type of gas that reacted with the color changing indicator based on the color detected by the sensors, wherein each color may be associated with a different type of gas.

Abstract: The present invention provides a method of rapid directed DNA evolution based on single-stranded combinatorial DNA mutant library. The single- and double-stranded mutant library are constructed either separately or simultaneously using editing primers that contain mutated nucleotides and are targeted to different regions of the parent DNA sequence. The mutant library is then inserted into expression vectors and mutants with desired property are obtained by high throughput screening. Evolution of promoters, enzymes and metabolic pathways were successfully achieved using this method and mutants with excellent properties were obtained. The method of the present invention is simple, rapid, and efficient. It can be used for directed evolution of regulatory sequence such as promoters and ribosome binding sites, and is especially suitable for introducing diverse mutations into protein encoding genes, leading to rapid directed evolution of gene of interest.

Abstract: The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.

Abstract: A write-assisted memory includes a pre-charge assist circuit that assists the pre-charge of the power supply voltage on a power supply lead for an accessed memory cell in a bit-line-multiplexed group of memory cells subsequent to a write-assist period by coupling charge from the power supply leads for the remaining non-accessed memory cells in the bit-line-multiplexed group of memory cells.

Abstract: A method for determining and identifying corrosion protective layers that provide corrosion protection against crude oils and crude oil fractions is disclosed. The method identifies naturally occurring constituents in crude oils that indirectly provide corrosion protection. A method assessing the potential of these constituents is also disclosed. The method includes exposing metal coupons with the crude oil or crude fraction of interest at the expected operating temperature of concern. The corrosion potential assessment further analyzes the exposed coupons with transmission electron microscopy and an additional high temperature exposure that challenges the tenacity of the protection offered by the corrosion protective layer.

Abstract: A gas detection apparatus includes a housing which carries a plurality of light emitting diodes which are coupled in parallel and which emit substantially the same wavelength of radiant energy. A closed loop control circuit maintains the radiant energy output of the diodes at substantially a predetermined value. The radiant light radiant light and a sample of a gas of interest are directed to a sensing position at which a gas responsive tape is positioned. Reflected light from the tape is detected at a sensor displaced from the tape. A light collecting element can be positioned between the coupled diodes and the sensing position.

Abstract: This invention provides an inkjet printing positioning device and control method thereof. The inkjet printing positioning device comprises: base; linear guide rail fixedly mounted on the base; inkjet unit, moving in matched manner with the linear guide rail; ball screw, driving the inkjet unit to move linearly when being rotated; servomotor, for driving the ball screw to rotate; zero position detecting device, mounted in a position on the moving travel route of the inkjet unit from the initial position to the printing area, and for sending zero position signal when detects that the inkjet unit is passing by; controller connecting the zero position detecting device and the servomotor, and for controlling the servomotor to drive the inkjet unit to move from the current position to the printing area and locate there, and controlling the servomotor to drive the inkjet unit to move predetermined displacement upon receipt of zero position signal.

Abstract: Methods of screening compounds, active compositions, pharmaceutical compositions, methods of treating disorders, methods of preventing disorders, and kits to screen a library of compounds, are disclosed.

Abstract: Various integrated circuits and methods of operating the integrated circuits are disclosed. The integrated circuit may include a circuit having one or more electrical parameters resulting from process variations during the manufacture of the integrated circuit, and a voltage source configured to supply a voltage to the circuit to power the circuit, wherein the voltage source is further configured to adjust the voltage as a function of the one or more electrical parameters.

Abstract: An integrated circuit is disclosed. The integrated circuit includes a plurality of bit-cells arranged to store data. The integrated circuit also includes a sensor configured to generate an output for determining whether the bit-cells are operating at a process corner. The sensor comprises the same circuitry as the bit-cells.

Abstract: The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.

Abstract: An integrated circuit is disclosed. The integrated circuit includes a plurality of bit-cells arranged to store data. The integrated circuit also includes a sensor configured to generate an output for determining whether the bit-cells are operating at a process corner. The sensor comprises the same circuitry as the bit-cells.

Abstract: A write-assisted memory includes a pre-charge assist circuit that assists the pre-charge of the power supply voltage on a power supply lead for an accessed memory cell in a bit-line-multiplexed group of memory cells subsequent to a write-assist period by coupling charge from the power supply leads for the remaining non-accessed memory cells in the bit-line-multiplexed group of memory cells.

Abstract: The disclosure relates to an apparatus for deactivating one or more predecoded address lines of a memory circuit in response to one or more of the predecoded address lines being activated upon powering on of at least a portion of the apparatus. In particular, the apparatus includes a memory device; an address predecoder configured to activate one or more of a plurality of predecoded address lines based on an input address, wherein the plurality of predecoded address lines are coupled to the memory device for accessing one or more memory cells associated with the one or more activated predecoded address lines; and a power-on-reset circuit configured to deactivate one or more of the predecoded address lines in response to the one or more of the predecoded address lines being activated upon powering on the at least portion of the apparatus.

Abstract: Various integrated circuits and methods of operating the integrated circuits are disclosed. The integrated circuit may include a circuit having one or more electrical parameters resulting from process variations during the manufacture of the integrated circuit, and a voltage source configured to supply a voltage to the circuit to power the circuit, wherein the voltage source is further configured to adjust the voltage as a function of the one or more electrical parameters.

Abstract: The disclosure relates to an apparatus for deactivating one or more predecoded address lines of a memory circuit in response to one or more of the predecoded address lines being activated upon powering on of at least a portion of the apparatus. In particular, the apparatus includes a memory device; an address predecoder configured to activate one or more of a plurality of predecoded address lines based on an input address, wherein the plurality of predecoded address lines are coupled to the memory device for accessing one or more memory cells associated with the one or more activated predecoded address lines; and a power-on-reset circuit configured to deactivate one or more of the predecoded address lines in response to the one or more of the predecoded address lines being activated upon powering on the at least portion of the apparatus.

Abstract: A method for determining and identifying corrosion protective layers that provide corrosion protection against crude oils and crude oil fractions is disclosed. The method identifies naturally occurring constituents in crude oils that indirectly provide corrosion protection. A method assessing the potential of these constituents is also disclosed. The method includes exposing metal coupons with the crude oil or crude fraction of interest at the expected operating temperature of concern. The corrosion potential assessment further analyzes the exposed coupons with transmission electron microscopy and an additional high temperature exposure that challenges the tenacity of the protection offered by the corrosion protective layer.