Abstract: A self-reconfiguring genome uses a cassette having operons or DNA sequences that code for guide RNA, reverse transcriptase, donor RNA, and a CRISPR cleavage enzyme. A self-reconfiguring genome may be based on lambda recombineering of in situ generated oligonucleotides. A method for programmable self-modification of a cellular genome includes transcribing guide RNA from a self-reconfiguring cassette, associating the transcribed guideRNA with the CRISPR enzyme, intercalating a region of complimentary sequence within an integration site of the genome, cutting upstream of a PAM site within the integration site; transcribing the donorRNA, translating donorRNA to double-stranded DNA, and recombining the double-stranded DNA via homologous recombination at the cut site of the integration site.

Abstract: In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3? or 5? protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3? or 5? associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.

Abstract: A device for controlling electron flow is provided. The device comprises a cathode, an elongate electrical conductor embedded in a diamond substrate, an anode, and a control electrode provided on the substrate surface for modifying the electric field in the region of the end of the conductor. A method of manufacturing the device is also provided.

Abstract: A device for controlling electron flow is provided. The device comprises a cathode, an elongate electrical conductor embedded in a diamond substrate, an anode, and a control electrode provided on the substrate surface for modifying the electric field in the region of the end of the conductor. A method of manufacturing the device is also provided.

Abstract: A device for controlling electron flow is provided. The device comprises a cathode, an elongate electrical conductor embedded in a diamond substrate, an anode, and a control electrode provided on the substrate surface for modifying the electric field in the region of the end of the conductor. A method of manufacturing the device is also provided.

Abstract: A device for controlling electron flow is provided. The device comprises a cathode, an elongate electrical conductor embedded in a diamond substrate, an anode, and a control electrode provided on the substrate surface for modifying the electric field in the region of the end of the conductor. A method of manufacturing the device is also provided.

Abstract: The disclosure provides an approach for mimicking human camera operation with an autonomous camera system. In one embodiment, camera planning is formulated as a supervised regression problem in which an automatic broadcasting application receives one video input captured by a human-operated camera and another video input captured by a stationary camera with a wider field of view. The automatic broadcasting application extracts feature vectors and pan-tilt-zoom states from the stationary camera and the human-operated camera, respectively, and learns a regressor which takes as input such feature vectors and outputs pan-tilt-zoom settings predictive of what the human camera operator would choose. The automatic broadcasting application may then apply the learned regressor on newly captured video to obtain planned pan-tilt-zoom settings and control an autonomous camera to achieve the planned settings to record videos which resemble the work of a human operator in similar situations.

Abstract: The disclosure provides an approach for predicting trajectories for real-time capture of video and object tracking, while adhering to smoothness constraints so that predictions are not excessively jittery. In one embodiment, a temporally consistent search and learn (TC-SEARN) algorithm is applied to train a regressor for camera planning. A automatic broadcasting application first receives video input captured by a human-operated camera and another video input captured by a stationary camera with a wide field of view. The automatic broadcasting application extracts feature vectors and pan-tilt-zoom states from the stationary camera input and human-operated camera input, respectively. The automatic broadcasting application further applies the TC-SEARN algorithm to learn a sequential regressor for predicting camera trajectories, based on the extracted feature vectors and pan-tilt-zoom states.

Abstract: There is provided a system including a memory and a processor configured to receive a first image depicting a first object and a second image depicting a second object, divide the first image into a first plurality of patches and the second image into a second plurality of patches, extract plurality of feature vectors from each of the first plurality of patches and a second plurality of feature vectors from the second plurality of patches, determine dissimilarities based on a plurality of patch metrics, each patch dissimilarity measure being a dissimilarity between corresponding patches of the first plurality of patches and the second plurality of patches, compute an image dissimilarity between the first image and the second image based on an aggregate of the plurality of patch dissimilarity measures, evaluate the image dissimilarity to determine a probability of whether the first object and the second object are the same.

Abstract: A self-reconfiguring genome uses a cassette having operons or DNA sequences that code for guide RNA, reverse transcriptase, donor RNA, and a CRISPR cleavage enzyme. A self-reconfiguring genome may be based on lambda recombineering of in situ generated oligonucleotides. A method for programmable self-modification of a cellular genome includes transcribing guide RNA from a self-reconfiguring cassette, associating the transcribed guideRNA with the CRISPR enzyme, intercalcating a region of complimentary sequence within an integration site of the genome, cutting upstream of a PAM site within the integration site; transcribing the donorRNA, translating donorRNA to double-stranded DNA, and recombining the double-stranded DNA via homologous recombination at the cut site of the integration site.

Abstract: The disclosure provides an approach for mimicking human camera operation with an autonomous camera system. In one embodiment, camera planning is formulated as a supervised regression problem in which an automatic broadcasting application receives one video input captured by a human-operated camera and another video input captured by a stationary camera with a wider field of view. The automatic broadcasting application extracts feature vectors and pan-tilt-zoom states from the stationary camera and the human-operated camera, respectively, and learns a regressor which takes as input such feature vectors and outputs pan-tilt-zoom settings predictive of what the human camera operator would choose. The automatic broadcasting application may then apply the learned regressor on newly captured video to obtain planned pan-tilt-zoom settings and control an autonomous camera to achieve the planned settings to record videos which resemble the work of a human operator in similar situations.

Abstract: Devices and methods integrate nanopore and microfluidic technologies for recording molecular characteristics of individual molecules such as, for example, biomolecules. Devices comprise a first substrate comprising a microchannel, a second substrate comprising a microchannel, the second substrate positioned below the first substrate, and a membrane having a thickness of about 0.3 nm to about 1 nm and comprising at least one nanopore, the membrane positioned between the first substrate and the second substrate, wherein a single nanopore of the membrane is constructed and arranged for electrical and fluid communication between the microchannel of the first substrate and the microchannel of the second substrate. To mitigate the effect of errors that occur during de novo DNA synthesis, longer DNA molecules are typically synthesized from shorter oligonucleotides by polymerase construction and amplification (PCA), or by other methods.

Abstract: Approaches are described for discovering a formation associated with an agent group engaging in an activity over a window of time. A formation analysis system computes first and second results for an objective function based on first and second sets of role assignments for each agent in the agent group at first and second moments in time, respectively. The formation analysis system iterates by: replacing the first set of role assignments with the second set of role assignments, and determining whether completion criteria have been met based at least in part on comparing the first result with the second result. If the completion criteria have not been met, then the formation analysis system replaces the second set of role assignments with a third set of role assignments that associate each agent in the first agent group with a different role assignment in the third set of role assignments at a third moment in time.

Abstract: A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

Abstract: The disclosure provides an approach for mimicking human camera operation with an autonomous camera system. In one embodiment, camera planning is formulated as a supervised regression problem in which an automatic broadcasting application receives one video input captured by a human-operated camera and another video input captured by a stationary camera with a wider field of view. The automatic broadcasting application extracts feature vectors and pan-tilt-zoom states from the stationary camera and the human-operated camera, respectively, and learns a regressor which takes as input such feature vectors and outputs pan-tilt-zoom settings predictive of what the human camera operator would choose. The automatic broadcasting application may then apply the learned regressor on newly captured video to obtain planned pan-tilt-zoom settings and control an autonomous camera to achieve the planned settings to record videos which resemble the work of a human operator in similar situations.

Abstract: In some aspects, the instant disclosure relates to the multiplexed encryption of information on nucleic acid molecules. In some aspects, the instant disclosure relates to a method of secure communication of information disseminated across at least one nucleic acid molecule, the method comprising (a) obtaining a modified keyboard comprising a personalized platform for translating text into a nucleic acid sequence; (b) translating a quantum of information into a nucleic acid message sequence using the modified keyboard of (a); and, (c) obtaining an at least one nucleic acid molecule, each molecule comprising: (i) the complete or a portion of the nucleic acid message sequence, and (ii) at least one contiguous stretch of randomized variable nucleic acid sequence flanking and/or inserted into the message sequence, thereby producing a nucleic acid molecule or a set of nucleic acid molecules containing the entire quantum of information.

Abstract: The disclosure relates to the use of steganographic methods to camouflage information encoded on nucleic acids. Specifically, the method comprising preparing a pool of recombinant nucleic acid constructs, wherein at least one of the constnjcts comprises the nucleic acid sequence to be camouflaged and wherein the pool is heterogeneous with respect to the orientation of the nucleic acid sequence to be camouflaged, and the information is camouflaged using genetic recombination.

Abstract: There is provided a system including a memory and a processor configured to receive a first image depicting a first object and a second image depicting a second object, divide the first image into a first plurality of patches and the second image into a second plurality of patches, extract plurality of feature vectors from each of the first plurality of patches and a second plurality of feature vectors from the second plurality of patches, determine dissimilarities based on a plurality of patch metrics, each patch dissimilarity measure being a dissimilarity between corresponding patches of the first plurality of patches and the second plurality of patches, compute an image dissimilarity between the first image and the second image based on an aggregate of the plurality of patch dissimilarity measures, evaluate the image dissimilarity to determine a probability of whether the first object and the second object are the same.

Abstract: Techniques are described to characterize motion patterns of a group of agents engaging in an activity. An analysis system receives input data associated with spatial and temporal information of at least one element of interest associated with the activity, where the object of interest may be a ball, person, animal or any other object in motion. The analysis system partitions the input data into a plurality of spatiotemporal segments and generates one or more representations of one or more sets of segments of the plurality of spatiotemporal segments based on one or more criteria. The analysis system computes a metric, such as an entropy value, for each of the one or more representations. Partial tracing data, such as ball movements in a sporting event, may be created using an inexpensive input device, such as a tablet computer, making the disclosed techniques available for a wide range of events and activities.

Abstract: To generate a media presentation of a live event, a user interface is coupled to at least three cameras that share substantially the same vantage point. One of the cameras (e.g., a context camera) provides a context view of the event that is displayed on a screen of the user interface. The views of the other two cameras are superimposed onto the context view to define sub-portions that are visually demarcated within the context view. Based on user interaction, the user interface can switch between the cameras views and control the cameras to capture different portions of the context view. Based the image data captured by the views of the cameras within the context view, the user interface generates a media presentation that may be broadcast to multiple viewers.