Abstract: In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3? or 5? protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3? or 5? associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.

Abstract: A device and method for receiving first detection information for a plurality of objects, the first detection information relating to a first characteristic of the objects, receiving second detection information for the objects, the second detection information relating to a second characteristic of the objects, determining first detections based upon the first detection information and second detections based upon the second detection information, formulating trellis graphs for the first and second detections, each trellis graph graphs including corresponding nodes at a plurality of time frames and determining a tracking of a selected one of the objects based upon a simultaneous shortest path for the selected object through both the first and second trellis graphs based upon a first path through the first trellis graph, a second path through the second trellis graph, and sidekick information.

Abstract: In a method for generating a long nucleic acid molecule, nucleic acids immobilized on a surface and having overlapping complementary sequences is released into solution. The overlapping complementary sequences are hybridized to form hybridized nucleic acids, followed by extension or ligation of the hybridized nucleic acids to synthesize the long nucleic acid molecule. The nucleic acids may comprise first and second series of nucleic acids having redundant overlapping sequences, wherein nucleic acids from the first and second series are complementary to each other. The complementary nucleic acids are hybridized to form the hybridized nucleic acids. The generated long nucleic acid molecule may have a predetermined sequence element, and it may be introduced into a system wherein the predetermined sequence element is required for replication, such that replication of the synthesized long nucleic acid molecule is indicative of the presence of the predetermined sequence element in the long nucleic acid molecule.

Abstract: The disclosure provides an approach for mimicking human camera operation with an autonomous camera system. In one embodiment, camera planning is formulated as a supervised regression problem in which an automatic broadcasting application receives one video input captured by a human-operated camera and another video input captured by a stationary camera with a wider field of view. The automatic broadcasting application extracts feature vectors and pan-tilt-zoom states from the stationary camera and the human-operated camera, respectively, and learns a regressor which takes as input such feature vectors and outputs pan-tilt-zoom settings predictive of what the human camera operator would choose. The automatic broadcasting application may then apply the learned regressor on newly captured video to obtain planned pan-tilt-zoom settings and control an autonomous camera to achieve the planned settings to record videos which resemble the work of a human operator in similar situations.

Abstract: The disclosure provides an approach for predicting trajectories for real-time capture of video and object tracking, while adhering to smoothness constraints so that predictions are not excessively jittery. In one embodiment, a temporally consistent search and learn (TC-SEARN) algorithm is applied to train a regressor for camera planning. A automatic broadcasting application first receives video input captured by a human-operated camera and another video input captured by a stationary camera with a wide field of view. The automatic broadcasting application extracts feature vectors and pan-tilt-zoom states from the stationary camera input and human-operated camera input, respectively. The automatic broadcasting application further applies the TC-SEARN algorithm to learn a sequential regressor for predicting camera trajectories, based on the extracted feature vectors and pan-tilt-zoom states.

Abstract: Methods and systems for measuring group behavior are provided. Group behavior of different groups may be measured objectively and automatically in different environments including a dark environment. A uniform visible signal comprising images of members of a group may be obtained. Facial motion and body motions of each member may be detected and analyzed from the signal. Group behavior may be measured by aggregating facial motions and body motions of all members of the group. A facial motion such as a smile may be detected by using the Fourier Lucas-Kanade (FLK) algorithm to register and track faces of each member of a group. A flow-profile for each member of the group is generated. Group behavior may be further analyzed to determine a correlation of the group behavior and the content of the stimulus. A prediction of the general public's response to the stimulus based on the analysis of the group behavior is also provided.

Abstract: There is provided a system for tracking objects. The system includes a processor and a memory for storing a plurality of sensory data frames. The processor determines a first hypothesized location for each of the objects in each of the plurality of sensory data frames. For each of the plurality of sensory data frames, the processor determines probabilities that the first hypothesized location of each of the objects in a sensory data frame of the plurality of sensory data frames is the same as the first hypothesized location of another object in an adjacent sensory data frame. The processor computes a first optimal trajectory for each of the objects using an algorithm based on the probabilities, checks the first optimal trajectory for each of the objects, and accepts or rejects the first optimal trajectory for each of the objects.

Abstract: Approaches are described for assigning roles to agents in a group of agents engaging in an activity. An assignment analysis system receives a first set of detections, where each detection in the first set of detections comprises a physical location. The assignment analysis system defines an exemplar formation comprising an arrangement of each role in a set of roles. The assignment analysis system calculates a first cost function between at least one detection in the first set of detections and at least one role in the set of roles. The assignment analysis system generates a first set of permutations based on the first cost function. The assignment analysis system assigns a first role in the set of roles to a first detection in the first set of detections based on the first set of permutations.

Abstract: Approaches are described for discovering a formation associated with an agent group engaging in an activity over a window of time. A formation analysis system computes first and second results for an objective function based on first and second sets of role assignments for each agent in the agent group at first and second moments in time, respectively. The formation analysis system iterates by: replacing the first set of role assignments with the second set of role assignments, and determining whether completion criteria have been met based at least in part on comparing the first result with the second result. If the completion criteria have not been met, then the formation analysis system replaces the second set of role assignments with a third set of role assignments that associate each agent in the first agent group with a different role assignment in the third set of role assignments at a third moment in time.

Abstract: There is provided a system for tracking objects. The system includes a processor and a memory for storing a plurality of sensory data frames. The processor determines a first hypothesized location for each of the objects in each of the plurality of sensory data frames. For each of the plurality of sensory data frames, the processor determines probabilities that the first hypothesized location of each of the objects in a sensory data frame of the plurality of sensory data frames is the same as the first hypothesized location of another object in an adjacent sensory data frame. The processor computes a first optimal trajectory for each of the objects using an algorithm based on the probabilities, checks the first optimal trajectory for each of the objects, and accepts or rejects the first optimal trajectory for each of the objects.

Abstract: Devices and methods integrate nanopore and microfluidic technologies for recording molecular characteristics of individual molecules such as, for example, biomolecules. Devices comprise a first substrate comprising a microchannel, a second substrate comprising a microchannel, the second substrate positioned below the first substrate, and a membrane having a thickness of about 0.3 nm to about 1 nm and comprising at least one nanopore, the membrane positioned between the first substrate and the second substrate, wherein a single nanopore of the membrane is constructed and arranged for electrical and fluid communication between the microchannel of the first substrate and the microchannel of the second substrate. To mitigate the effect of errors that occur during de novo DNA synthesis, longer DNA molecules are typically synthesized from shorter oligonucleotides by polymerase construction and amplification (PCA), or by other methods.

Abstract: To generate a media presentation of a live event, a user interface is coupled to at least three cameras that share substantially the same vantage point. One of the cameras (e.g., a context camera) provides a context view of the event that is displayed on a screen of the user interface. The views of the other two cameras are superimposed onto the context view to define sub-portions that are visually demarcated within the context view. In one embodiment, only one of views is visually demarcated in the context view at any given time. Based on user interaction, the user interface can switch between the cameras views and control the cameras to capture different portions of the context view. Based the image data captured by the views of the cameras within the context view, the user interface generates a media presentation that may be broadcast to multiple viewers.

Abstract: Close proximity, including conversations at closer than three (3) feet apart, facilitates disease transfer via airborne pathogens. This transfer can be mitigated by the present invention, which is directed towards methods, systems and apparatuses to produce a predictably shaped and minimally intrusive air barrier that may comprise an airborne disinfectant, to divert and or render harmless and to divert and negate close proximity airborne pathogens, such as influenza and severe acute respiratory syndrome, etc., and other airborne particulates transferred from an infected person towards the face of an unfortunate recipient at normal conversational distances apart from each other, and/or to divert and render harmless such airborne particulates.

Abstract: Approaches are described for assigning roles to agents in a group of agents engaging in an activity. An assignment analysis system receives a first set of detections, where each detection in the first set of detections comprises a physical location. The assignment analysis system defines an exemplar formation comprising an arrangement of each role in a set of roles. The assignment analysis system calculates a first cost function between at least one detection in the first set of detections and at least one role in the set of roles. The assignment analysis system generates a first set of permutations based on the first cost function. The assignment analysis system assigns a first role in the set of roles to a first detection in the first set of detections based on the first set of permutations.

Abstract: A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

Abstract: Close proximity, including conversations at closer than three (3) feet apart, facilitates disease transfer via airborne pathogens. This transfer can be mitigated by the present invention, which is directed towards methods, systems and apparatuses to produce a predictably shaped and minimally intrusive air barrier that may comprise an airborne disinfectant, to divert and or render harmless and to divert and negate close proximity airborne pathogens, such as influenza and severe acute respiratory syndrome, etc., and other airborne particulates transferred from an infected person towards the face of an unfortunate recipient at normal conversational distances apart from each other, and/or to divert and render harmless such airborne particulates.

Abstract: A self-reconfiguring genome uses a cassette having operons or DNA sequences that code for guide RNA, reverse transcriptase, donor RNA, and a CRISPR cleavage enzyme. A self-reconfiguring genome may be based on lambda recombineering of in situ generated oligonucleotides. A method for programmable self-modification of a cellular genome includes transcribing guide RNA from a self-reconfiguring cassette, associating the transcribed guideRNA with the CRISPR enzyme, intercalcating a region of complimentary sequence within an integration site of the genome, cutting upstream of a PAM site within the integration site; transcribing the donorRNA, translating donorRNA to double-stranded DNA, and recombining the double-stranded DNA via homologous recombination at the cut site of the integration site.

Abstract: Methods and systems for measuring group behavior are provided. Group behavior of different groups may be measured objectively and automatically in different environments including a dark environment. A uniform visible signal comprising images of members of a group may be obtained. Facial motion and body motions of each member may be detected and analyzed from the signal. Group behavior may be measured by aggregating facial motions and body motions of all members of the group. A facial motion such as a smile may be detected by using the Fourier Lucas-Kanade (FLK) algorithm to register and track faces of each member of a group. A flow-profile for each member of the group is generated. Group behavior may be further analyzed to determine a correlation of the group behavior and the content of the stimulus. A prediction of the general public's response to the stimulus based on the analysis of the group behavior is also provided.

Abstract: The invention concerns image processing that includes the estimation of the optical depths of points in image data in order to enhance the image data. In particular but not limited to, from the optical depth noise present in the image due to aerosol in the scene, such as fog or haze, can be reduced. The invention concerns determining a first cost for each point independently based on a determined metric (60) and then a second cost based on an expected correlation in neighboring points (70). The two costs are then optimized (80) to determine an estimated optical depth for each point (80) that is then used to reduce the effect of aerosol in the image (84). Applications of the invention include, but is not limited to, surveillance systems so that objects in the scene of the image can be better identified once the image data has been enhanced to reduce the estimated noise. Aspects of the invention include a method, software, computer apparatus and a field programmable gate array.