Patents by Inventor Peter A. Sims

Peter A. Sims has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • INTER ORGAN PLATFORM FOR MICROPHYSIOLOGICAL SYSTEM ON A CHIP
    Publication number: 20250092345
    Abstract: A modular bioreactor system is provided including a platform having a seat to receive a modular tissue chamber, and one or more platform interfacial members; and a releasable modular tissue chamber including a bottom surface, an open top, and sidewalls defining a well therein, the releasable modular tissue chamber configured to be received by the seat of the platform, the tissue chamber further including an interfacial member configured to releasably connect the modular tissue chamber to the platform, wherein a tissue chamber contains human bone marrow (engineered using iPS cell derived osteoblasts, osteoclasts, endothelial cells, supporting mesenchymal cells, and hematopoietic cells in bone matrix) infused by primary leukemia cells
    Type: Application
    Filed: July 18, 2022
    Publication date: March 20, 2025
    Inventors: Gordana Vunjak-Novakovic, Andrea Califano, Peter Sims, Alan Chramiec, Ece Ozturk, Kacey Ronaldson, Daniel Naveed Tavakol, Adolfo Ferrando, Jessie Brown, Keith YEAGER
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20240117427
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: December 4, 2023
    Publication date: April 11, 2024
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20240093294
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: November 28, 2023
    Publication date: March 21, 2024
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • High-throughput single molecule protein identification
    Patent number: 11927593
    Abstract: The present invention is methods and assays for identifying single proteins from a sample, without the use of affinity reagents. The methods and assays combine endopeptidase-based component of conventional peptide mapping with single molecule labeling and a microreactor array platform. The invention also includes kits for performing the methods and assays.
    Type: Grant
    Filed: May 18, 2020
    Date of Patent: March 12, 2024
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventor: Peter A. Sims
  • Single cell nucleic acid detection and analysis
    Patent number: 11866781
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: May 14, 2021
    Date of Patent: January 9, 2024
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single cell nucleic acid detection and analysis
    Patent number: 11834712
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: March 2, 2023
    Date of Patent: December 5, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • RNA printing and sequencing devices, methods, and systems
    Patent number: 11788120
    Abstract: Described herein are devices, systems, and methods for trapping single-cell lysates in sealed, microwells capable of printing RNA on glass or capturing RNA on beads. These provide efficient, inexpensive manipulation of RNA from individual cells suitable for single-cell transcriptomics on a large scale. Also described are dual barcode capture beads and merged barcode capture beads.
    Type: Grant
    Filed: November 27, 2018
    Date of Patent: October 17, 2023
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Peter A. Sims, Sayantan Bose, Jinzhou Yuan
  • INTER-ORGAN PLATFORM WITH TISSUE-SPECIFIC NICHES FOR A MICROPHYSIOLOGICAL SYSTEM ON A CHIP
    Publication number: 20230287316
    Abstract: Disclosed are systems and methods for culturing systemic bioengineered tumor models, including osteosarcoma cells or breast adenocarcinoma cells derived from patients. The model includes the tumor cells on host tissues such as bone, liver, lung, and heart tissue, wherein the bone, liver, lung, and heart tissue are separated by endothelial barriers. Beneath the endothelial barriers the tissues are connected via circulation containing secreted factors and cells (e.g., tumor/immune cells).
    Type: Application
    Filed: November 22, 2022
    Publication date: September 14, 2023
    Inventors: Gordana Vunjak-Novakovic, Andrea Califano, Peter Sims, Alan Chramiec, Ece Ozturk, Kacey Ronaldson, Keith Yeager, Diogo Teles
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20230212668
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: March 2, 2023
    Publication date: July 6, 2023
    Inventors: Xiaoliang Sunney Xie, Katsuyki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single cell nucleic acid detection and analysis
    Patent number: 11629379
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: April 13, 2022
    Date of Patent: April 18, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single cell nucleic acid detection and analysis
    Patent number: 11608527
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: April 13, 2022
    Date of Patent: March 21, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • SYSTEMS, METHODS, AND COMPOSITIONS FOR GENERATING MULTI-OMIC INFORMATION FROM SINGLE CELLS
    Publication number: 20220403465
    Abstract: Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with/without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.
    Type: Application
    Filed: November 13, 2020
    Publication date: December 22, 2022
    Inventors: Peter A. SIMS, Timothy R. OLSEN
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20220259652
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: April 13, 2022
    Publication date: August 18, 2022
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20220251646
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: April 13, 2022
    Publication date: August 11, 2022
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single cell nucleic acid detection and analysis
    Patent number: 11352669
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: June 30, 2021
    Date of Patent: June 7, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single cell nucleic acid detection and analysis
    Patent number: 11286523
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: January 28, 2020
    Date of Patent: March 29, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • RNA Printing and Sequencing Devices, Methods, and Systems
    Publication number: 20220064717
    Abstract: Many important biological questions demand single-cell transcriptions on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. Described herein are devices, systems and methods for trapping single-cell lysates in sealed, microwells capable of priming RNA on glass or capturing RNA on beads.
    Type: Application
    Filed: September 21, 2021
    Publication date: March 3, 2022
    Applicant: The Trustees of Columbia University in the City of New York
    Inventors: Peter A. SIMS, Sayantan BOSE, Jinzhou YUAN
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20210381045
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: August 24, 2021
    Publication date: December 9, 2021
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20210324469
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: June 30, 2021
    Publication date: October 21, 2021
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20210277466
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: May 14, 2021
    Publication date: September 9, 2021
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia