Abstract: The present invention relates to a method for transfecting a eukaryotic cell, wherein a compound comprising at least one saccharide and at least one protein and optionally a salt and/or a component stabilizing pH is brought into contact with a nucleic acid and a transfection reagent, a complex of nucleic acid and transfection reagent is formed by means of interacting with the composition, and the complex is transfected into a eukaryotic cell.

Abstract: A method of preparing an additive suspension circuit for a hard disk drive suspension includes electrodepositing an alloy of copper using an electrolytic bath containing copper sulfate, tin, iron, sulfuric acid, and hydrochloric acid, using pulsed current of about 10-45 amperes per square foot.

Abstract: The invention relates to a method for inhibiting the expression of a target gene in a eukaryotic cell. The method includes the following steps: a) providing at least one eukaryotic cell, the cell being capable of RNA interference, b) transfecting the eukaryotic cell with a composition that includes a genetic construct that includes an siRNA tag, and a target gene that forms a transcription unit together with the siRNA tag, and c) introducing at least one siRNA that is complementary to the siRNA tag of the transfected genetic construct to inhibit the expression of the target gene.

Abstract: Polymer particles, comprising a) at least one sparingly soluble insecticide from the group consisting of fipronil, allethrin, alpha-cypermethrin, beta-cyfluthrin, bifenthrin, bioallethrin, 4-chloro-2-(2-chloro-2-methylpropyl)-5-[(6-iodo-3-pyridinyl)methoxy]-3(2H)-pyridazinone (CAS-RN: 120955-77-3), chlorantraniliprole, chlorfenapyr, cyantraniliprole, cyfluthrin, cyhalothrin, cypermethrin, deltamethrin, etofenprox, fenoxycarb, flufenoxuron, hydramethylnon, imidacloprid, indoxacarb, metaflumizone, permethrin, pyriproxifen, tebufenozide and tralomethrin and b) at least one water-insoluble polymer, are suitable for improving the soil mobility of the sparingly soluble insecticide(s).

Abstract: A dual stage actuator (DSA) suspension has a single microactuator such as a PZT element on one side of central longitudinal axis of the suspension, and a pseudo symmetry structure formed or affixed on the other side of the central longitudinal axis opposite the PZT. The pseudo symmetry structure has mass and stiffness that mirrors the PZT, thus keeping the suspension mechanically balanced and symmetric about the longitudinal axis for improved suspension performance especially in a shock environment.

Abstract: The present invention pertains to the use of at least one abasic modification within the first 8 nucleotide positions of the 5? region of the antisense strand of a small interfering nucleic acid (siNA) molecule for reducing off-target effects. Provided are suitable modified siNAs, compositions and methods for producing respective siNAs, as well as kits comprising respective siNAs.

Abstract: A dual-stage actuator disk drive suspension load beam has a recessed cavity formed in it into which the microactuator motor is partially or completely placed. The cavity may be formed by partially etching the load beam during the same etching process that creates the load beam from a sheet of stainless steel material. The partially etched load beam having a cavity for the microactuator motor has the advantages of a lower profile, improved inertial balancing of the suspension, and increased sway resonant frequency.

Abstract: The present invention provides a novel RNAi based selection system for selecting host cells that have incorporated an expression vector.

Abstract: A method of designing a hard disk drive suspension swage hub geometry for an actuator arm having upper and lower suspensions attached thereto, includes using a finite element analysis model to simulate the effects of swaging the suspensions to the actuator arm, identifying relevant geometric parameters of the suspensions, for each suspensions performing a linear regression to fit an equation containing the geometric parameters to a normal force of the suspension to the actuator arm after swaging according to the fine element analysis, identifying a desired range of differences between the normal forces, using the desired difference range, and solving for values of geometric parameters to produce a set of target design parameters for the suspension swage hubs.

Abstract: An electrical power management apparatus and method in which a public power grid, a renewable energy source, an energy storage element and a domestic power supply network are interconnected through a controllable switch which connects the various components together in combinations which are advantageous to sensed power load and consumption circumstances.

Abstract: A gimbal, a disk drive suspension that includes the gimbal, and a related method of manufacture, wherein the gimbal is configured to be coupled to a slider having a disk drive read-write transducer. The gimbal includes one or more strut(s) having an insulating layer that includes a first surface, and a supporting layer that includes a plurality of segments and that is coupled to the insulating layer's first surface.

Abstract: The invention pertains to the use of an apoptosis inducing combination of at least a. a first expression modulating compound silencing the expression of at least a first target gene involved in apoptosis and b. a second expression modulating compound silencing the expression of at least a second target gene involved in apoptosis as a positive control in expression modulating assays. Also provided are suitable methods, kits and compositions.

Abstract: Shock performance and stiffness of hard disk drive lifters are enhanced by extending one or more portions of a load beam in a dimension normal to the load beam plane. A load beam in a high shock suspension system comprises a planar body having transverse members extending between longitudinal rails. One embodiment comprises a lifter integral to the load beam, extending distally, and comprising a rib having a conic cross section and a lifting tab having upward curving edges. Another embodiment comprises rails having edges bending upward at 90 degrees, separated by a first width at a proximal end of the body, and tapering to a second width at a distal end of the body. A narrow lifter having upward curving edges is displaced between the rails, and extends distally from the body. Stiffeners extend from the rails and connect to an intermediate point on the lifter.

Abstract: The invention relates to a composition for inhibiting the expression of a target gene in a eukaryotic cell by RNA interference, the composition comprising a genetic construct and the target gene being introduced into the eukaryotic cell by means of said construct, and the construct having a first target gene and a first siRNA tag. The invention also relates to a composition for inhibiting the expression of one or more target genes in a eukaryotic cell by RNA interference. The composition comprises at least two genetic constructs and the target genes are introduced into the eukaryotic cell by means of said constructs, a) the first construct containing a first target gene and a first siRNA tag and b) the second construct containing a second target gene and a second siRNA tag, and the first and the second siRNA tag having different nucleic acid sequences. The invention finally relates to a method for inhibiting the expression of a target gene in a eukaryotic cell.

Abstract: A computer-implemented method may include receiving a first input selection from a user made while in a page of an application, providing an indication to the user of one or more views on the page of the application that are user configurable, in response to receiving the first input selection, receiving a second input selection from the user of one of the views, and instantiating a user interface (UI) configuration tool to enable the user to configure the selected view, in response to receiving the second input selection.

Abstract: A disk drive suspension has a mount plate, an outwardly tapered load beam supported by the mount plate and having a base portion, a hinge portion, a beam portion having a first face with turned edges and a flexure supported by the beam portion distally of said beam portion face, and a generally congruent tapered stiffener having a central portion of a given height that is concave and open to the beam portion. The stiffener has left and right flange portions extending laterally from the central portion to the beam portion upturned edges to locate said central portion centrally on the beam portion face.

Abstract: A wireless disk drive suspension and method of manufacture in which curvilinear deflections of the plastic film and conductive traces relative to the flexure metal layer provide for containment of the strains accompanying pitch and roll adjustment of the flexure and allow adjustment free of adjusting difficulties and spring back compromise of the adjustment. The curvilinear deflection of the laminate toward the flexure central longitudinal axis and an accompanying bringing in of the flexure frame struts provides a less wide flexure that can more closely approach a disk drive hub for greater capacity in a disk drive.

Abstract: A mounting assembly for securing a disk drive to a frame of a computer is provided herein. The mounting assembly includes three rigid mounts and a single flexible mount. Each rigid mount rigidly secures the drive housing to the frame and prevents degradation of performance of the disk drive. The flexible mount diminishes the level of vibration transferred from the frame to drive housing. Further, the flexible mount facilitates flexing of the drive housing intermediate the flexible mount and the rigid mounts. This reduces the effects of a shock pulse to the disk drive and inhibits head slap between a transducer head and a storage disk.

Abstract: In a method for automatically gutting fish of the catfish species, the belly cavity is opened around the anal orifice either before or after opening the gills by severing the gullet and opening the blood stream, and the contents of the belly cavity are removed by pushing these from one of the openings of the cavity out of the other. In a machine for carrying out this method, the fish are conveyed sideways by a recessed conveyor. The belly cavity is opened in the vicinity of the anus by separating away the cartilaginous plate carrying the ventral fins. The contents of the ventral cavity are removed by a rotary body rotating synchronously with the conveyor and having tools successively arranged in a cantilevered helix around its circumference which comprise a clearing tool with scraping segments, a brush tool, a high-pressure spray device and a cutting tool for slitting open the belly cavity along its length.

Abstract: Silicon wafers and a process for the production thereof which produces was having intrinsic gettering action and on which thin oxide layers with high breakdown strength can be produced. During the production, the wafers are subjected to a treatment which smooths the wafer surface, for example, by repolishing, after the intrinsic getters have been produced in the interior of the wafer. Oxide layers subsequently deposited are outstanding for an increased breakdown strength compared with untreated wafers.