Patents by Inventor Peter S. Gagnon

Peter S. Gagnon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Dissociation of product-complexed contaminants in chromatography
    Patent number: 9193760
    Abstract: The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule.
    Type: Grant
    Filed: September 20, 2011
    Date of Patent: November 24, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Enhanced purification of antibodies and antibody fragments by apatite chromatography
    Patent number: 9127042
    Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.
    Type: Grant
    Filed: December 2, 2011
    Date of Patent: September 8, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • METHODS FOR PURIFICATION OF RECOMBINANT AAV VECTORS
    Publication number: 20150024467
    Abstract: Provided herein are methods for the purification of recombinant adeno-associated virus (rAAV) vectors that can be used for gene transfer and specifically for gene therapy or vaccination. Recombinant AAV vectors of the invention are substantially free of in-process impurities, including production components such as cellular nucleic acids, cellular proteins, helper virus, and media components.
    Type: Application
    Filed: December 16, 2011
    Publication date: January 22, 2015
    Applicant: GENZYME CORPORATION
    Inventors: Pauline McLean Quigley Sheldon, Peter S. Gagnon, Gina Nichols, Barbara A. Thorne
  • DNA removal in target molecule purification
    Patent number: 8911992
    Abstract: Removal of genomic DNA from biological samples using cation exchange is described.
    Type: Grant
    Filed: April 28, 2011
    Date of Patent: December 16, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Chromatography purification of antibodies
    Patent number: 8877904
    Abstract: Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: November 4, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Peter S. Gagnon, Hong Chen, Russ Frost
  • REMOVAL OF VIRUCIDAL AGENTS FROM BIOMOLECULE PREPARATIONS
    Publication number: 20130267690
    Abstract: Methods, compositions and kits for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by hydroxyapatite (HT) or fluorapatite (FT) that is treated with a polycationic agent. In some embodiments, the antibodies are treated with a polycationic agent that is also a virucidal agent prior to purification.
    Type: Application
    Filed: September 18, 2012
    Publication date: October 10, 2013
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY
    Publication number: 20130210164
    Abstract: Provided are methods and compositions for reducing or eliminating pH excursions in cation and anion exchange chromatography.
    Type: Application
    Filed: February 8, 2013
    Publication date: August 15, 2013
    Applicant: BIO-RAD LABORATORIES, INC.
    Inventor: Peter S. Gagnon
  • DNA REMOVAL IN TARGET MOLECULE PURIFICATION
    Publication number: 20120276623
    Abstract: Removal of genomic DNA from biological samples using cation exchange is described.
    Type: Application
    Filed: April 28, 2011
    Publication date: November 1, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • CHROMATOGRAPHY PURIFICATION OF ANTIBODIES
    Publication number: 20120264915
    Abstract: Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step.
    Type: Application
    Filed: April 30, 2012
    Publication date: October 18, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Peter S. Gagnon, Hong Chen, Russ Frost
  • Dissociation of Product-complexed Contaminants in Chromatography
    Publication number: 20120252098
    Abstract: The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule.
    Type: Application
    Filed: September 20, 2011
    Publication date: October 4, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Chromatography purification of antibodies
    Patent number: 8188242
    Abstract: Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step.
    Type: Grant
    Filed: April 7, 2009
    Date of Patent: May 29, 2012
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Peter S. Gagnon, Hong Chen, Russ Frost
  • ENHANCED PURIFICATION OF ANTIBODIES AND ANTIBODY FRAGMENTS BY APATITE CHROMATOGRAPHY
    Publication number: 20120077961
    Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.
    Type: Application
    Filed: December 2, 2011
    Publication date: March 29, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Enhanced purification of antibodies and antibody fragments by apatite chromatography
    Patent number: 8093364
    Abstract: Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed.
    Type: Grant
    Filed: January 16, 2009
    Date of Patent: January 10, 2012
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • ENHANCED CAPACITY AND PURIFICATION OF PROTEIN BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS
    Publication number: 20110263823
    Abstract: This invention relates to the use of mixed mode chromatography for purification of a protein from a mixture containing other materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies or other proteins suitable for in vivo applications.
    Type: Application
    Filed: July 8, 2011
    Publication date: October 27, 2011
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
    Patent number: 8017740
    Abstract: This invention relates to the use of mixed mode chromatography for purification of at least one intact non-aggregated antibody from a mixture containing intact non-aggregated antibodies and undesirable materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies suitable for in vivo applications.
    Type: Grant
    Filed: February 19, 2010
    Date of Patent: September 13, 2011
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
    Patent number: 7999085
    Abstract: This invention relates to the use of mixed mode chromatography for purification of a protein from a mixture containing other materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies or other proteins suitable for in vivo applications.
    Type: Grant
    Filed: April 10, 2009
    Date of Patent: August 16, 2011
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • ENHANCED ANTIBODY AGGREGATE REMOVAL WITH CAPTO ADHERE IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS
    Publication number: 20110166332
    Abstract: The invention relates to a method for separating at least one non-aggregated protein from a liquid protein preparation by contacting said preparation with a multimodal anion exchanger in the presence of protein-excluded zwitterions at a concentration of 0.25 to 2.5 M.
    Type: Application
    Filed: August 28, 2009
    Publication date: July 7, 2011
    Applicant: GE HEALTHCARE BIO-SCIENCES AB
    Inventor: Peter S. Gagnon
  • ENHANCED CAPACITY AND PURIFICATION OF ANTIBODIES BY MIXED MODE CHROMATOGRAPHY IN THE PRESENCE OF AQUEOUS-SOLUBLE NONIONIC ORGANIC POLYMERS
    Publication number: 20100145029
    Abstract: This invention relates to the use of mixed mode chromatography for purification of at least one intact non-aggregated antibody from a mixture containing intact non-aggregated antibodies and undesirable materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies suitable for in vivo applications.
    Type: Application
    Filed: February 19, 2010
    Publication date: June 10, 2010
    Applicant: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
    Patent number: 7691980
    Abstract: This invention relates to the use of mixed mode chromatography for purification of at least one intact non-aggregated antibody from a mixture containing intact non-aggregated antibodies and undesirable materials, including fragmented or aggregated antibodies, host cell proteins, DNA, endotoxin, and/or virus. This invention further relates to the integration of such a method into a multi-step procedure with other fractionation methods for purification of antibodies suitable for in vivo applications.
    Type: Grant
    Filed: January 7, 2008
    Date of Patent: April 6, 2010
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Peter S. Gagnon
  • ENHANCED PROTEIN AGGREGATE REMOVAL BY MIXED MODE CHROMATOGRAPHY ON HYDROPHOBIC INTERACTION MEDIA IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS
    Publication number: 20100069617
    Abstract: This invention relates to methods for enhancing purification of proteins such as monoclonal antibodies by chromatography on carboxyl group-containing HIC supports in the presence of zwitterions that are excluded from protein surfaces. In certain embodiments, the invention may permit more effective separation of non-aggregated protein from aggregated protein.
    Type: Application
    Filed: September 3, 2009
    Publication date: March 18, 2010
    Applicant: GE HEALTHCARE BIO-SCIENCES AB
    Inventor: PETER S. GAGNON