Abstract: Provided are fusion proteins including an amino acid sequence of an ectodomain of Spike protein of a coronavirus, such as SARS-CoV-2, joined to an amino acid sequence of a ferritin subunit polypeptide. Nanoparticles including such fusion proteins, with surface-exposed trimers of the ectodomain of the Spike protein of the coronavirus, are also provided. Also provided are nucleic acids and vectors encoding the fusion proteins, cells containing such nucleic acid and vectors, immunogenic compositions including the fusion proteins, the nanoparticles, or the vectors, as well as corresponding methods and kits.

Abstract: A method for providing user feedback and recommendations regarding the likelihood that a fund transfer transaction will be completed can include receiving a first message and a second message, from an external device, that includes first transaction data and second transaction data for a proposed transaction. The method can further include generating a first assessment and a second assessment, responsive to receiving the first message and the second message and based at least in part on the first transaction data and the second transaction data, of the likelihood that the transaction will be successful if initiated. The method can further include providing user feedback to the external device to indicate the likelihood. Other systems, apparatuses, and methods are also described.

Abstract: In one embodiment, a system is provided. The system includes a memory device for storing templates; and a processing device, operatively coupled to the memory device, to select a template in view of user input. The template includes one or more embedded data fields associated with a study. A query comprises a tree of operators in view of the embedded data fields is derived. The set of operators being adapted to evaluate events data stored in a longitudinal database. Results corresponding to the query are generated by applying at least one operator of the tree of operators to the events data. The results including a time series of outcomes related to the events data. Thereafter, the template is populated with a least a portion of the results for presentation in connection with the study.

Abstract: Provided are methods of producing antigenic proteins and antigenic protein complexes and compositions and vaccines produced from such methods. Also provided are methods of immunizing a subject using such compositions and vaccines. The antigenic proteins and protein complexes of this disclosure are useful as vaccine immunogens that can direct the immune system of a subject immunized with such vaccine immunogens to generate antibodies against a specific region, or epitope, of a protein that is known to be productive or neutralizing in the case of an infection. Also provided are methods of screening for B cells expressing antibodies that bind specifically to a particular, desired epitope (target region). Compositions and kits comprising one or more antigenic protein, protein complexes, binding partners, and polynucleotides encoding any thereof, are also described.

Abstract: The disclosure describes a method for managing user accounts. The method may include receiving, by a computing device, a user identifier associated with a user of an automated teller machine and a location identifier associated with the automated teller-machine. The method may further include determining, by the computing device and based on the user identifier, user data that includes at least one of user financial data, user preferences data, or user personal data. The method may further include determining, by the computing device and based on the location identifier and the user data, a recommended action for the user of the automated teller machine. The method may further include sending, by the computing device and to the automated teller machine, information associated with the recommended action to be displayed to the user on a display of the automated teller machine.

Abstract: Variants of human PD-1 comprising one or more of amino acid substitutions in residues corresponding to N74, T76 and A132 of SEQ ID NO:1 are described. Also described are structures, obtained using X-ray crystallography, of the human PD-1/PD-L2 complex and mutant PD-1 variants. The structures of human PD-1 described in the present disclosure are useful in drug discovery, including small-molecule drug discovery. Accordingly, methods of using the structures in drug discovery are also described.

Abstract: In one embodiment, a method is provided. The method comprises receiving a query comprising a time period associated with events data stored in a longitudinal database. Using a processing system, a variable data structure is identified in view of the query. The variable data structure is used to identify attributes associated with the events data from the longitudinal database. A tree of operators is generated to evaluate the events data in view of the variable data structure and the time period. Thereupon, results corresponding to the query are provided by applying at least one operator of the tree of operators to the variable data structure. The results include a time series of outcomes related to the events data.

Abstract: Variants of human PD-1 comprising one or more of amino acid substitutions in residues corresponding to N74, T76 and A132 of SEQ ID NO:1 are described. Also described are structures, obtained using X-ray crystallography, of the human PD-1/PD-L2 complex and mutant PD-1 variants. The structures of human PD-1 described in the present disclosure are useful in drug discovery, including small-molecule drug discovery. Accordingly, methods of using the structures in drug discovery are also described.

Abstract: In one embodiment, a method of assembling a longitudinal database is provided. The method comprises receiving, using a processor of a data storage unit, medical data streams from a plurality of sources. The medical data streams include patient data related to a plurality of medical events. A first date range that indicates a time span associated with the patient is identified in view of the patient data. The medical events are normalized into a plurality of linking keys based on the first date range. The keys are adapted to longitudinally link each medical event in a sequence of occurrence. Each key of comprises an offset from a start date of the first date range for a corresponding medical event. The medical events are compressed in view of the keys. The compressing generates a location index for each medical event associated with the keys based on a corresponding offset.

Abstract: A method, system, and apparatus for associating a patient with a bed or a room includes sensing wireless information transmitted by a transmitter worn by the patient, such as an RFID transmitter of a wristband, and relying on automatic communication between the bed, a bed status computer, and an admission, discharge and tracking (ADT) computer to verify the patient-to-room or patient-to-bed association. The verification is achieved without the need for any manual data entry at the bed or at the bed status computer. The bed status computer stores bed data sent from the bed until the verification is made and then the bed status computer transmits the stored bed data to an electronic medical records (EMR) computer.

Abstract: Five-Helix protein, which comprises the three N-helices and at least two, but not three, of the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, such as amino acid residue linkers, is disclosed. Six-Helix protein, which includes the three N-helices and the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, is also disclosed.

Abstract: Inhibitors of HIV membrane fusion and a method of identifying drugs or agents which inhibit binding of the N-helix coiled-coil and the C helix of HIV gp41 envelope protein.

Abstract: Described herein are chimeric peptides comprising a soluble trimeric coiled-coil and all or a portion of the N-peptide region of HIV gp41. These molecules are stable, trimeric coiled-coils that inhibit HIV entry into cells, such as human cells. Such peptides can be further assessed to demonstrate their ability to serve as potent anti-HIV therapeutic molecules and thus, as therapeutic molecules or drugs.

Abstract: Five-Helix protein, which comprises the three N-helices and at least two, but not three, of the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, such as amino acid residue linkers, is disclosed. Six-Helix protein, which includes the three N-helices and the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, is also disclosed.

Abstract: Human scFvs are disclosed which interact with a conformational epitope along the pre-hairpin, N-helix coiled coil structure within the heptad repeat 1 (HR1) region of gp41 of HIV. These antibodies, as well as IgG conversions, are shown to neutralize diverse HIV isolates. Isolated nucleic acid molecules are also disclosed which encode relevant portions of these antibodies, as well as the purified forms of the expressed antibodies or relevant antibody fragments, such as VH and VL chains. The antibody compositions disclosed within this specification may provide for a therapeutic treatment against HIV infection by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of HIV infection. These antibodies will also be useful in assays to identify HIV antiviral compounds as well as allowing for the identification of candidate HIV vaccines, such as HIV peptide vaccines.

Abstract: A method of evaluating a water sample for the presence or possible future presence of nitrification comprises obtaining data values of a number of parameters, processing the data values to determine correlation coefficients, to identify any linear dependencies, to standardize the scales, evaluating the data values over a plurality of proliferation time periods and neuron numbers, calculating MSEs and R2's from the evaluations, and estimating a valid likelihood of nitrification of the water sample. A method of evaluating a water sample for the presence or possible future presence of nitrification, comprises obtaining data values of a number of parameters, statistically pre-processing the data values and supplying the pre-processed data values to a neural network. Apparatus, media and processors which are used in performing such methods.

Abstract: Five-Helix protein, which comprises the three N-helices and at least two, but not three, of the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, such as amino acid residue linkers, is disclosed. Six-Helix protein, which includes the three N-helices and the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, is also disclosed.

Abstract: Described herein are chimeric peptides comprising a soluble trimeric coiled-coil and all or a portion of the N-peptide region of HIV gp41. These molecules are stable, trimeric coiled-coils that inhibit HIV entry into cells, such as human cells. Such peptides can be further assessed to demonstrate their ability to serve as potent anti-HIV therapeutic molecules and thus, as therapeutic molecules or drugs.

Abstract: Described are the crystal structure of the ?-helical domain of the gp41 component of HIV-1 envelope glycoprotein which represents the core of fusion-active gp41, methods of identifying and designing drugs which inhibit gp41 function and drugs which do so.

Abstract: A method of evaluating a water sample for the presence or possible future presence of nitrification comprises obtaining data values of a number of parameters, processing the data values to determine correlation coefficients, to identify any linear dependencies, to standardize the scales, evaluating the data values over a plurality of proliferation time periods and neuron numbers, calculating MSEs and R2's from the evaluations, and estimating a valid likelihood of nitrification of the water sample. A method of evaluating a water sample for the presence or possible future presence of nitrification, comprises obtaining data values of a number of parameters, statistically pre-processing the data values and supplying the pre-processed data values to a neural network. Apparatus, media and processors which are used in performing such methods.