Abstract: An in-line gravity filtration device for biological fluids such as blood or blood products is disclosed. The device includes a series of channels formed downstream of filtration elements. The channels are defined in cross sectional area by the distance between the filtration elements and their bottoms. The channels are configured to form flow paths to an outlet port so that air within the channels is forced downstream through the outlet port thereby minimizing air being trapped on the downstream side of the device. For optimum performance, the cross section area of a single continuous channel, or the sum of the cross sectional area of parallel channels, leading to a single outlet port, should not exceed the cross sectional area of the outlet port.

Abstract: A filtration device for separating filtrate and concentrate from a solution that contains a dead stop feature. The dead stop volume is determined by the location of an outlet port. The apparatus includes a housing with a means for collecting filtrate and a filter unit insertable within the housing. The filter unit contains a semipermeable membrane thereon for separating filtrate from concentrate.

Abstract: An in-line liquid filtration device useable for filtration of blood, blood products or the like includes a housing having an inlet port, an outlet port, at least one filter element disposed in the housing between the inlet port and outlet port so as to filter liquid which flows into the filtration device via the inlet port. The filter element divides the housing into a first chamber and a second chamber. The device allows gases to vent the filtration device through the outlet port. The means may include a flow deflector within the first chamber and/or the second chamber. The means may also include a channel, preferably spiral, within either the first chamber and/or second chamber. The filtration device allows air therein to be purged downstream into either an air collecting bag or into the blood receiving bag without the manipulation of the height of the filtration device or the blood receiving bag.

Abstract: A liquid filtration device useable for removing leukocytes from blood contains a first chamber which is in fluid flow relationship with a second chamber. Filtration elements separate the first chamber from the second chamber so that liquid flowing from the first chamber is filtered thereby prior to entry into the second chamber. A passage leads from the second chamber into the first chamber and a hydrophobic filter may be used to prevent liquid from the first chamber from flowing through the passage into the second chamber while allowing air to flow therethrough. An outlet is located in the second chamber, preferably at the bottom thereof. A second outlet may be located within the second chamber and may be placed in fluid flow relationship therewith by a conduit or the like.

Abstract: An in-line gravity filtration device for biological fluids such as blood or blood products is disclosed. The device includes a series of channels formed downstream of filtration elements. The channels are defined in cross sectional area by the distance between the filtration elements and their bottoms. The channels are configured to form flow paths to an outlet port so that air within the channels is forced downstream through the outlet port thereby minimizing air being trapped on the downstream side of the device. For optimum performance, the cross section area of a single continuous channel, or the sum of the cross sectional area of parallel channels, leading to a single outlet port, should not exceed the cross sectional area of the outlet port.

Abstract: The vacuum filtration device has a disposable filter funnel and a disposable filtrate receptacle. The funnel and receptacle are used with a reusable base that is connected to a vacuum source. When the receptacle and funnel are mounted on the base the vacuum is applied through a connecting passage to the receptacle to draw the filtrate from the funnel into the receptacle.

Abstract: An in-line liquid filtration device useable for filtration of blood, blood products or the like includes a housing having an inlet port, an outlet port, at least one filter element disposed in the housing between the inlet port and outlet port so as to filter liquid which flows into the filtration device via the inlet port. The filter element divides the housing into a first chamber and a second chamber. The device allows gases to vent the filtration device through the outlet port. The means may include a flow deflector within the first chamber and/or the second chamber. The means may also include a channel, preferably spiral, within either the first chamber and/or second chamber. The filtration device allows air therein to be purged downstream into either an air collecting bag or into the blood receiving bag without the manipulation of the height of the filtration device or the blood receiving bag.

Abstract: The filtration method involves processing biological liquid from an enclosed flexible supply bag to a filtration media within a processing system wherein the flow of biological liquid within the system is restricted to allow the biological liquid to back up above the level of a port located upstream within the system and preventing gas from entering into the processing system through the port by maintaining the level of biological liquid above the port until the flow of biological liquid from the flexible supply bag ceases. The system automatically allows gas to enter into the system through the port when the flow of biological liquid ceases thereby draining the biological liquid into a receiving bag. The flow may be restricted by a narrow cross-sectional area within the processing system and the port may be covered by a hydrophobic filter media.

Abstract: An in-line liquid filtration device useable for filtration of blood, blood products or the like includes a housing having an inlet port, an outlet port, at least one filter element disposed in the housing between the inlet port and outlet port so as to filter liquid which flows into the filtration device via the inlet port. The filter element divides the housing into a first chamber and a second chamber. The device allows gases to vent the filtration device through the outlet port. The means may include a flow deflector within the first chamber and/or the second chamber. The means may also include a channel, preferably spiral, within either the first chamber and/or second chamber. The filtration device allows air therein to be purged downstream into either an air collecting bag or into the blood receiving bag without the manipulation of the height of the filtration device or the blood receiving bag.

Abstract: A liquid filtration device useable for removing leukocytes from blood is disclosed. Located in-line on the upstream side of the filtration device is a vent device which is configured to allow liquid being filtered to flow therethrough and creates a pressure head sufficient to prevent air from entering the vent until the fluid flow therethrough stops and thereby allows air to automatically enter the vent to allow the air to drain liquid through the filtration device. The vent minimizes air entering the filtration system during the flow of liquid flow therethrough while automatically allowing air to enter the system and thereby drain the system when the flow of fluid stops.

Abstract: A liquid filtration device useable for removing leukocytes from blood contains a first chamber which is in fluid flow relationship with a second chamber. Filtration elements separate the first chamber from the second chamber so that liquid flowing from the first chamber is filtered thereby prior to entry into the second chamber. A passage leads from the second chamber into the first chamber and a hydrophobic filter may be used to prevent liquid from the first chamber from flowing through the passage into the second chamber while allowing air to flow therethrough. An outlet is located in the second chamber, preferably at the bottom thereof. A second outlet may be located within the second chamber and may be placed in fluid flow relationship therewith by a conduit or the like.

Abstract: The vacuum filtration device has a disposable filter funnel and a disposable filtrate receptacle. The funnel and receptacle are used with a reusable base that is connected to a vacuum source. When the receptacle and funnel are mounted on the base the vacuum is applied through a connecting passage to the receptacle to draw the filtrate from the funnel into the receptacle.

Abstract: A filtration device for separating filtrate and concentrate from a solution may contain a variable dead stop feature. The apparatus includes a housing with a means for collecting filtrate and a multiple sided filter unit insertable within the housing. The filter unit contains a semipermeable membrane thereon for separating filtrate from concentrate and a means for directing filtrate to a discharge within the housing. The apparatus also contains a means for preventing concentrate within the housing from escaping through the discharge opening of the housing.

Abstract: A sparger useful for introducing gas into a liquid is composed of one or more gas-permeable members positioned between a gas inlet means and a gas distribution means. A method and apparatus for culturing cells, eukaryotic cells and hybridomas in particular, wherein circulation of a liquid medium contained in the apparatus is achieved by gas introduced into the liquid medium by means of the aforementioned sparger.