Abstract: This disclosure provides, among other things, a transgenic animal and a method of using the same to make antibodies that have a common light chain. In certain embodiments, the transgenic animal may comprising a genome comprising a common light chain transgene, wherein the common light chain transgene comprises a non-immunoglobulin light-chain promoter and a common light-chain coding sequence. In certain embodiments, the common light chain is constitutively expressed.

Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.

Abstract: A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.

Abstract: The present invention is transgenic chickens obtained from long-term cultures of avian PGCs and techniques to produce and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. This invention includes compositions comprising long-term cultures of PGCs and offspring derived from them that are genetically modified.

Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

Abstract: The present invention is long-term cultures of avian PGCs and techniques to produce germline chimeric and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. These germline chimeric birds do not have substantial contributions of PGC-derived phenotypes in somatic cells or tissues. This invention includes compositions comprising long-term cultures of PGCs that can be genetically modified by gene targeting, that can accept large amounts of foreign DNA and that contribute to the germline of recipient embryos.

Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

Abstract: The invention relates to transgenic birds capable of producing chimeric immunoglobulins, with a combination of human and avian sequence, in their B cells. In some embodiments, the birds are chickens. When challenged with an antigen, the transgenic avians produce antigen-specific functional antibodies. The invention also relates to light chain immunoglobulin transgenes for making such transgenic avians, as well as methods and vectors for disrupting endogenous immunoglobulin loci in birds.

Abstract: The invention relates to transgenic birds capable of producing chimeric immunoglobulins, with a combination of human and avian sequence, in their B cells. In some embodiments, the birds are chickens. When challenged with an antigen, the transgenic avians produce antigen-specific functional antibodies. The invention also relates to light chain immunoglobulin transgenes for making such transgenic avians, as well as methods and vectors for disrupting endogenous immunoglobulin loci in birds.

Abstract: The present invention is transgenic chickens obtained from long-term cultures of avian PGCs and techniques to produce and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. This invention includes compositions comprising long-term cultures of PGCs and offspring derived from them that are genetically modified.

Abstract: The present invention is transgenic chickens obtained from long-term cultures of avian PGCs and techniques to produce and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. This invention includes compositions comprising long-term cultures of PGCs that can be genetically modified by gene targeting, that can accept large amounts of foreign DNA and that contribute to the germline of recipient embryos.

Abstract: Human antibodies are produced in transgenic chickens having functional genetic components of the human immune system stably integrated in the genome. Techniques are described to integrate unrearranged human heavy and light chain immunoglobulin loci into the genome of the transgenic chicken. The functional description of the endogenous immunoglobulin loci yields an animal whose antibody repertoire in response to antigen is fully human. In the preferred embodiment, the human immunoglobulin locus is at least as large as the native human locus and exhibits functional class switching to yield IgG isotype antibodies. In addition to monoclonal antibodies secreted from immortalized B cell populations, polyclonal antibodies may be obtained from eggs laid by the transgenic chickens of the invention.

Abstract: The present invention is long-term cultures of avian PGCs and techniques to produce germline chimeric and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs produce germline chimeric birds. These germline chimeric birds do not have PGC derived somatic cells or tissues. This invention includes compositions comprising long-term cultures of PGCs that can be genetically modified by gene targeting, that can accept large amounts of foreign DNA and that contribute to the germline of recipient embryos.

Abstract: A conveying system comprises a frame and an endless belt moveable relative to the frame. The belt comprises a first fabric layer having an inner surface and an outer surface that at least partially forms an upper surface of the belt. A composition comprising a flame retardant is adhered to the inner surface of the first fabric layer such that the composition at least partially forms the inner surface of the first fabric layer. A second fabric layer has an inner surface and an outer surface that at least partially forms a bottom surface of the belt. A composition comprising polyurethane is adhered to the inner surface of the second fabric layer such that the composition at least partially forms the inner surface of the second fabric layer. An at least substantially solid lubricant composition is impregnated in the second fabric layer and at least partially forms the bottom surface of the belt. The belt is supported on a slider bed for movement across the slider bed.

Abstract: A door panel comprises a fabric carcass, including a first fabric layer having an inner surface and an outer surface, a second fabric layer having an inner surface and an outer surface, and a composition comprising polyurethane, the composition disposed between the inner surface of the first fabric layer and the inner surface of the second fabric layer for joining the first fabric layer and the second fabric layer. A first foam polymeric layer has an inner surface adhered to the outer surface of the first fabric layer such that an outer surface of the first foam polymeric layer at least partially forms an outer surface of the door panel. A second foam polymeric layer has an inner surface adhered to the outer surface of the second fabric layer such that an outer surface of the second foam polymeric layer at least partially forms an inner surface of the door panel.

Abstract: Human antibodies are produced in transgenic chickens having functional genetic components of the human immune system stably integrated in the genome. Techniques are described to integrate unrearranged human heavy and light chain immunoglobulin loci into the genome of the transgenic chicken. The functional description of the endogenous immunoglobulin loci yields an animal whose antibody repertoire in response to antigen is fully human. In the preferred embodiment, the human immunoglobulin locus is at least as large as the native human locus and exhibits functional class switching to yield IgG isotype antibodies. In addition to monoclonal antibodies secreted from immortalized B cell populations, polyclonal antibodies may be obtained from eggs laid by the transgenic chickens of the invention.

Abstract: A conveying system comprises a frame and an endless belt moveable relative to the frame. The belt comprises a first fabric layer having an inner surface and an outer surface that at least partially forms an upper surface of the belt. A composition comprising a flame retardant is adhered to the inner surface of the first fabric layer such that the composition at least partially forms the inner surface of the first fabric layer. A second fabric layer has an inner surface and an outer surface that at least partially forms a bottom surface of the belt. A composition comprising polyurethane is adhered to the inner surface of the second fabric layer such that the composition at least partially forms the inner surface of the second fabric layer. An at least substantially solid lubricant composition is impregnated in the second fabric layer and at least partially forms the bottom surface of the belt. The belt is supported on a slider bed for movement across the slider bed.

Abstract: A belt assembly is provided for use as an endless belt traveling relative to a frame structure. The belt assembly comprises a first fabric layer and a second fabric layer having an outer surface that at least partially forms a bottom surface of the belt. A composition comprising polyvinyl chloride is adhered to the inner surface of the first fabric layer. A composition comprising polyurethane is adhered to the inner surface of the second fabric layer. A coating composition comprising polyvinyl chloride is disposed between the inner surfaces of the first fabric layer and the second fabric layer for joining the first fabric layer and the second fabric layer. An at least substantially solid lubricant composition is impregnated in the second fabric layer and at least partially forms the bottom surface of the belt.

Abstract: A belt assembly is provided for use as an endless belt traveling relative to a frame structure. The belt assembly comprises a first plain woven fabric layer having an inner surface and an outer surface that at least partially forms an upper surface of the belt. The first fabric layer includes synthetic yarns having a denier of at least about 1000. A second lofty woven fabric layer has an inner surface and an outer surface that at least partially forms a bottom surface of the belt. The second fabric layer includes an effective amount of textured synthetic yarns and/or synthetic staple filament yarns. An adhesive composition comprising an elastomeric material is adhered to the inner surface of the first fabric layer and the inner surface of the second fabric layer for joining the fabric layers.