Abstract: A device that can be used for in vivo fluorescence measurements by endoscopy or laparoscopy, including: an excitation light source illuminating an object, for example biological tissue, and inducing emission of emission light by the examined object, the emission light is, for example, a fluorescence light; a telemetry sensor emitting a telemetry light beam towards the object; a projector element to project the excitation beam and the telemetry beam directly on the object. The telemetry sensor can estimate a distance between the projector element and the object, which distance makes it possible to modulate intensity of the excitation light emitted by the object. When the object is biological tissue, this makes it possible to comply with illumination thresholds of biological tissue and to maintain an illumination of constant intensity. The projector element can be included at the end of a laparoscope or of an endoscope.

Abstract: The invention relates to a method allowing the use of the information accessible by fluorescence imaging to be optimized. For this purpose, it implements the combination of a protocol for calibration and synchronization of a pulsed light for exciting a fluorescent marker, with the operation in “rolling shutter” mode of a fluorescence camera. An appropriate correction factor allows the complete signal integrated by all of the photodiodes of the camera to be used so that no image is lost.

Abstract: A method for monitoring diffusion of a fluorescent marker within a region of biological tissue includes observing the marker's diffusion in a series of images captured by a camera and determining whether to display an image based at least in part on the evolution of the marker's spatial distribution as it perfuses through the tissue.

Abstract: The invention relates to a method allowing the use of the information accessible by fluorescence imaging to be optimized. For this purpose, it implements the combination of a protocol for calibration and synchronization of a pulsed light for exciting a fluorescent marker, with the operation in “rolling shutter” mode of a fluorescence camera. An appropriate correction factor allows the complete signal integrated by all of the photodiodes of the camera to be used so that no image is lost.

Abstract: Measuring fluorescence within a zone on a surface of biological tissue uses a probe that comprises first and second sensors that are constituents of corresponding first and second optical systems and that are fixed relative to each other. The first sensor is configured to detect fluorescent light emitted at said surface and the second sensor detects visible light. The measurement includes projecting a mark onto the zone, and, based on a position of an image of the mark on the second sensor, localizing and locating fluorescence detected by the first sensor in an image of the zone. The image is produced in visible light. The mark is a light mark that is detectable by said second sensor.

Abstract: Fluorescence imaging system for an operating theatre, comprising a device illuminating the operating theatre and emitting a white light, and a fluorescence imaging device. Said fluorescence imaging device comprises a light source emitting radiation for excitation of a fluorescent marker in a range of emission wavelengths of between 600 and 900 nm. The light emitted by the illuminating device is filtered by a low-pass filter, of which the cut-off wavelength is below the emission range of the fluorescent marker, but is nonetheless able to show an increase or fluctuations in the attenuation for wavelengths above the emission range of the fluorescent marker, the product of the attenuation of the filter of the detector and of the attenuation of the low-pass filter of the illuminating device leading to an attenuation by a factor of at least 106.

Abstract: Fluorescence imaging system for an operating theatre, comprising a device illuminating the operating theatre and emitting a white light, and a fluorescence imaging device. Said fluorescence imaging device comprises a light source emitting radiation for excitation of a fluorescent marker in a range of emission wavelengths of between 600 and 900 nm. The light emitted by the illuminating device is filtered by a low-pass filter, of which the cut-off wavelength is below the emission range of the fluorescent marker, but is nonetheless able to show an increase or fluctuations in the attenuation for wavelengths above the emission range of the fluorescent marker, the product of the attenuation of the filter of the detector and of the attenuation of the low-pass filter of the illuminating device leading to an attenuation by a factor of at least 106.

Abstract: A method for monitoring diffusion over time of a fluorescent marker that has been injected into a biological tissue at an injection-time includes using an excitation light source, exciting the fluorescent marker and, during an interval that begins after the injection-time and ends at an end-time, using a camera to acquire fluorescence images of an area of the biological tissue. This includes executing first and second image-acquisition sequences starting at different start-times after the injection time. The method includes comparing first and second images that result from having processed the image-acquisition sequences.

Abstract: The invention relates to a method allowing the use of the information accessible by fluorescence imaging to be optimized. For this purpose, it implements the combination of a protocol for calibration and synchronization of a pulsed light for exciting a fluorescent marker, with the operation in “rolling shutter” mode of a fluorescence camera. An appropriate correction factor allows the complete signal integrated by all of the photodiodes of the camera to be used so that no image is lost.

Abstract: A method for correcting a fluorescence image of an object, for example a biological tissue that can include fluorescent agents. The distance between a fluorescence probe generating the fluorescence image and the object examined is measured. This distance is used to apply a correction function to the fluorescence image. The method may find application in perioperative fluorescence imaging for diagnosis and monitoring of evolution of pathologies, for example of cancers.

Abstract: Measuring fluorescence within a zone on a surface of biological tissue uses a probe that comprises first and second sensors that are constituents of corresponding first and second optical systems and that are fixed relative to each other. The first sensor is configured to detect fluorescent light emitted at said surface and the second sensor detects visible light. The measurement includes projecting a mark onto the zone, and, based on a position of an image of the mark on the second sensor, localizing and locating fluorescence detected by the first sensor in an image of the zone. The image is produced in visible light. The mark is a light mark that is detectable by said second sensor.

Abstract: A device that can be used for in vivo fluorescence measurements by endoscopy or laparoscopy, including: an excitation light source illuminating an object, for example biological tissue, and inducing emission of emission light by the examined object, the emission light is, for example, a fluorescence light; a telemetry sensor emitting a telemetry light beam towards the object; a projector element to project the excitation beam and the telemetry beam directly on the object. The telemetry sensor can estimate a distance between the projector element and the object, which distance makes it possible to modulate intensity of the excitation light emitted by the object. When the object is biological tissue, this makes it possible to comply with illumination thresholds of biological tissue and to maintain an illumination of constant intensity. The projector element can be included at the end of a laparoscope or of an endoscope.

Abstract: A method for correcting a fluorescence image of an object, for example a biological tissue that can include fluorescent agents. The distance between a fluorescence probe generating the fluorescence image and the object examined is measured. This distance is used to apply a correction function to the fluorescence image. The method may find application in perioperative fluorescence imaging for diagnosis and monitoring of evolution of pathologies, for example of cancers.

Abstract: Fluorescence imaging system for an operating theatre, comprising a device illuminating the operating theatre and emitting a white light, and a fluorescence imaging device. Said fluorescence imaging device comprises a light source emitting radiation for excitation of a fluorescent marker in a range of emission wavelengths of between 600 and 900 nm. The light emitted by the illuminating device is filtered by a low-pass filter, of which the cut-off wavelength is below the emission range of the fluorescent marker, but is nonetheless able to show an increase or fluctuations in the attenuation for wavelengths above the emission range of the fluorescent marker, the product of the attenuation of the filter of the detector and of the attenuation of the low-pass filter of the illuminating device leading to an attenuation by a factor of at least 106.

Abstract: A system of analysis with the naked eye and by fluorescence of a field in an illuminated area comprising a periodically-excited first low-remanence white light illumination source; a second light source for exciting fluorescent elements located in said field, active at least during part of the time periods when the first source is off; and a fluorescence analysis device active during time periods when the first source is off and the second source is on.

Abstract: A system of analysis with the naked eye and by fluorescence of a field in an illuminated area comprising a periodically-excited first low-remanence white light illumination source; a second light source for exciting fluorescent elements located in said field, active at least during part of the time periods when the first source is off; and a fluorescence analysis device active during time periods when the first source is off and the second source is on.

Abstract: A system of analysis with the naked eye and by fluorescence of a field in an illuminated area comprising a periodically-excited first low-remanence white light illumination source; a second light source for exciting fluorescent elements located in said field, active at least during part of the time periods when the first source is off; and a fluorescence analysis device active during time periods when the first source is off and the second source is on.

Abstract: In the field of videoendoscopes for medical or industrial use, a videoendoscope has a bispectral optical head comprising a microcamera, white light lighting means and fluorescence light lighting means. The optical head is linked by appropriate electrical and optical connectors to the other elements of the videoendoscope. Preferably, it comprises inexpensive standard elements and is packaged in a single-use sterile packaging. There are different possible embodiments depending on whether the optical head comprises a single sensor or two sensors, depending on whether the device operates in continuous mode or in sequential mode and, finally, depending on whether the light sources are incorporated in the optical head or external thereto, the light being then routed by means of optical fibres.

Abstract: The invention relates to a method of localising a fluorophore (22) in a scattering medium (20), by means of a radiation source (8, 10) suited to emitting an excitation radiation of this fluorophore and detection means (4, 12) suited to measuring a fluorescence signal (?fluo) emitted by this fluorophore (22) comprising: a) for at least 3 different pairs of positions of the radiation source and detection means, an excitation by a radiation coming from the radiation source (8), and a detection by means (4) of detecting the fluorescence signal emitted by this fluorophore after this excitation, b) for each of these pairs, the identification of a surface on which the fluorophore is situated, or a volume comprising this surface and in which the fluorophore is situated, c) an estimation of the localisation of the fluorophore in its surrounding medium, by calculation of the intersection of the three surfaces, or if necessary a volume around this intersection.