Patents by Inventor Pil Seok Chae
Pil Seok Chae has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190241597Abstract: The present invention relates to a mesitylene-cored amphiphile, a method of preparing the same and a method of extraction, solubilization, stabilization, crystallization or analysis of a membrane protein using the same. When a mesitylene-cored compound according to the present invention is used, membrane proteins can be stored in a stable conformation for a long time in an aqueous solution in comparison with conventional compounds, by which the membrane proteins are usable in both functional and structural analyses thereof. The functional and structural analyses of membrane proteins are one of the fields that are most spotlighted in current biology and chemistry. The present invention can be used to research on a protein structure closely related to new drug development.Type: ApplicationFiled: August 31, 2016Publication date: August 8, 2019Applicant: Industry-University Cooperation Foundation Hanyang Erica CampusInventors: Pil Seok CHAE, Kyung Ho CHO
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Patent number: 10316057Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: July 22, 2014Date of Patent: June 11, 2019Assignee: Wisconsin Alumni Research FoundationInventors: Pil Seok Chae, Samuel Helmer Gellman
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Publication number: 20190169218Abstract: Disclosed are an amphipathic compound having a penta-saccharide hydrophilic group, a method of preparing the same, and a method of extracting, solubilizing, stabilizing, crystallizing or analyzing membrane proteins and membrane protein complexes using the same. In particular, since the compound has a high-density penta-saccharide hydrophilic group composed of five glucose units, the compound may have an excellent effect on crystallization of membrane proteins. In addition, since the hydrophilic group used in the amphipathic compound has a novel structure, the hydrophilic group may be applied to the development of various amphipathic molecules.Type: ApplicationFiled: April 14, 2017Publication date: June 6, 2019Inventors: Pil Seok CHAE, Muhammad EHSAN
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Publication number: 20190077822Abstract: The present invention relates to a mannitol-based amphipathic compound, a method of preparing the same, a method of extracting, solubilizing, stabilizing or crystallizing a membrane protein using the compound, and a method of analyzing a structure of the membrane protein under an electron microscope using the compound. When the mannitol-based compound according to the present invention is used, the membrane protein can be stably stored in an aqueous solution for a prolonged period of time and thus can be applied to analysis of functions and structures thereof. Since the analysis of the structures and functions of the membrane protein is one of the fields of most interest in biology and chemistry currently, and more than half of new drugs currently in development are targeting membrane proteins, the present invention is applicable to research on the structures of membrane proteins closely related to the development of the new drugs.Type: ApplicationFiled: December 14, 2015Publication date: March 14, 2019Inventors: Pil Seok Chae, Hazrat Hussain
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Publication number: 20180273570Abstract: The present invention relates to a xylene-based amphiphilic compound, a method for preparing the same, and a method for extracting, solubilizing, stabilizing or crystallizing a membrane protein using the same. By using the xylene-based compound according to the present invention, a membrane protein may be stably stored in an aqueous solution for a long time, and may be applied in functional and structural analyses. The structural and functional analysis of the membrane protein is one of the fields of highest interest in biology and chemistry, and the xylene-based compound according to the present invention can be applied in research on protein structure that is closely related to development of a new drug.Type: ApplicationFiled: April 15, 2016Publication date: September 27, 2018Inventors: Pil Seok Chae, Kyung Ho Cho
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Patent number: 9556217Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: November 3, 2015Date of Patent: January 31, 2017Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
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Patent number: 9540409Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: GrantFiled: August 7, 2013Date of Patent: January 10, 2017Assignee: WIsconsin Alumni Research FoundationInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasumssen
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Publication number: 20160060290Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: ApplicationFiled: November 3, 2015Publication date: March 3, 2016Inventors: Samuel Helmer GELLMAN, Pil Seok CHAE, Brian KOBILKA, Soren RASMUSSEN
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Patent number: 9255122Abstract: Disclosed are compounds and methods for manipulating proteins in general and membrane proteins in particular. The compounds can be prepared from cholic acid, deoxycholic acid, lithocholic acid, or derivatives thereof. The compounds typically possess critical micelle concentrations lower than those of known detergents such as CHAPS and CHAPSO. Accordingly, lower amounts of the compounds are required for effective solubilization of membrane proteins. The compounds can be used aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: December 1, 2014Date of Patent: February 9, 2016Assignee: Wisconsin Alumni Research FoundationInventors: Samuel Helmer Gellman, Pil Seok Chae
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Patent number: 9206221Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: March 14, 2013Date of Patent: December 8, 2015Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
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Publication number: 20150203441Abstract: Disclosed are compounds and methods for manipulating proteins in general and membrane proteins in particular. The compounds can be prepared from cholic acid, deoxycholic acid, lithocholic acid, or derivatives thereof. The compounds typically possess critical micelle concentrations lower than those of known detergents such as CHAPS and CHAPSO. Accordingly, lower amounts of the compounds are required for effective solubilization of membrane proteins. The compounds can be used aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: ApplicationFiled: December 1, 2014Publication date: July 23, 2015Inventors: Samuel Helmer Gellman, Pil Seok Chae
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Publication number: 20150011739Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: ApplicationFiled: July 22, 2014Publication date: January 8, 2015Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Pil Seok Chae, Samuel Helmer GELLMAN
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Patent number: 8877906Abstract: The invention provides amphiphiles for manipulating membrane proteins. The amphiphiles can feature carbohydrate-derived hydrophilic groups and branchpoints in the hydrophilic moiety and/or in a lipophilic moiety. Such amphiphiles are useful as detergents for solubilization and stabilization of membrane proteins, including photosynthetic protein superassemblies obtained from bacterial membranes.Type: GrantFiled: September 10, 2012Date of Patent: November 4, 2014Assignees: Wisconsin Aumni Research Foundation, UChicago Argonne, LLCInventors: Samuel Helmer Gellman, Pil Seok Chae, Phillip D. Laible, Marc J. Wander
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Patent number: 8815263Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: November 5, 2012Date of Patent: August 26, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Pil Seok Chae, Samuel Helmer Gellman
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Publication number: 20130324707Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: ApplicationFiled: August 7, 2013Publication date: December 5, 2013Applicants: The Board of Trustees of the Leland Stanford Junior University, Wisconsin Alumni Research FoundationInventors: Samuel Helmer GELLMAN, Pil Seok CHAE, Brian KOBILKA, Soren RASUMSSEN
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Publication number: 20130266656Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: ApplicationFiled: March 14, 2013Publication date: October 10, 2013Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
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Patent number: 8530631Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: GrantFiled: March 24, 2010Date of Patent: September 10, 2013Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
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Publication number: 20130001465Abstract: The invention provides amphiphiles for manipulating membrane proteins. The amphiphiles can feature carbohydrate-derived hydrophilic groups and branchpoints in the hydrophilic moiety and/or in a lipophilic moiety. Such amphiphiles are useful as detergents for solubilization and stabilization of membrane proteins, including photosynthetic protein superassemblies obtained from bacterial membranes.Type: ApplicationFiled: September 10, 2012Publication date: January 3, 2013Applicants: UCHICAGO ARGONNE, LLC, WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Samuel Helmer GELLMAN, Pil Seok CHAE, Phillip D. LAIBLE, Marc J. WANDER
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Patent number: 8263754Abstract: The invention provides amphiphiles for manipulating membrane proteins. The amphiphiles can feature carbohydrate-derived hydrophilic groups and branchpoints in the hydrophilic moiety and/or in a lipophilic moiety. Such amphiphiles are useful as detergents for solubilization and stabilization of membrane proteins, including photosynthetic protein superassemblies obtained from bacterial membranes.Type: GrantFiled: April 8, 2009Date of Patent: September 11, 2012Assignees: Wisconsin Alumni Research Foundation, UChicago Argonne, LLCInventors: Samuel Helmer Gellman, Pil Seok Chae, Philip D. Laible, Marc J. Wander
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Publication number: 20100311956Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: ApplicationFiled: March 24, 2010Publication date: December 9, 2010Applicant: WARF - Wisconsin Alumni Research FoundationInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen