Abstract: The present application disclosures a double production line and a rapid prefabrication process of a segmental beam. The double production line including two production machine and a track system provided on the construction ground; the production machine includes a fixed end mold, two side molds, a bottom mold trolley, a middle internal mold trolley and two side internal mold trolley; two side molds are positioned on two sides of the fixed end mold respectively, the fixed end mold and two side molds together define a pouring position with an end opening, two openings of the pouring position are arranged facing each other; the track system includes a transverse track and a longitudinal track communicated with each other, two pouring positions are both positioned in the extension path of the transverse track, and the longitudinal track is positioned between two pouring positions.

Abstract: In a method for sensing translocation of a molecule through a nanopore, a molecule in a first fluidic solution in a first fluidic reservoir that is in direct fluidic connection with the nanopore is directed to a nanopore inlet and translocated through the nanopore to a nanopore outlet and through a fluidic passage that is in direct fluidic connection with the nanopore outlet, to a second fluidic solution in a second fluidic reservoir disposed in direct fluidic connection with the fluidic passage. The fluidic passage has at least one fluidic section in which a length of the fluidic section is greater than a width of the fluidic section. Translocation of the molecule through the nanopore is sensed by measuring the electrical potential local to the fluidic passage during the translocation of the molecule.

Abstract: In a nanopore sensor, a nanopore disposed in a support structure has a nanopore diameter and nanopore resistance, RPore. A fluidic passage, disposed in fluidic connection between a first fluidic reservoir and the nanopore, has a cross-sectional extent, along at least a portion of the fluidic passage length, that is greater than the diameter of the nanopore and that is less than the fluidic passage length. The fluidic passage has a fluidic passage resistance, RFP, of at least about 10% of the nanopore resistance, RPore, and no more than about 10 times the nanopore resistance, RPore. The nanopore is disposed in fluidic connection between the fluidic passage and a second fluidic reservoir. At least one electrical transduction element is disposed at the fluidic passage and electrically connected to produce an indication of electrical potential local to the fluidic passage.

Abstract: Embodiments of a generative framework comprise cooperative learning of two generative flow models, in which the two models are iteratively updated based on the jointly synthesized examples. In one or more embodiments, the first flow model is a normalizing flow that transforms an initial simple density into a target density by applying a sequence of invertible transformations, and the second flow model is a Langevin flow that runs finite steps of gradient-based MCMC toward an energy-based model. In learning iterations, synthesized examples are generated by using a normalizing flow initialization followed by a short-run Langevin flow revision toward the current energy-based model. Then, the synthesized examples may be treated as fair samples from the energy-based model and the model parameters are updated, while the normalizing flow directly learns from the synthesized examples by maximizing the tractable likelihood. Also provided are both theoretical and empirical justifications for the embodiments.

Abstract: Devices for improved nanopore sensing are described. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. The structure can include an array of nanopore structures, each nanopore structure comprising a passage for fluid connection through the structure between the analyte reservoir and outlet chamber. Control terminals can be arranged for applying a control signal to alter the electrical potential difference across that nanopore structure. Some embodiments include an electronic circuit configured to detect a signal from an electrical transduction element at each nanopore structure. Additional structural features and methods of operating and making the devices are described.

Abstract: Provided herein are compositions, systems, and methods for treating wounds with the combination of statins and cholesterol to help prevent and reduce scar formation.

Abstract: A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

Abstract: A decorative lighting dimmer switch without influencing charging is provided. The decorative lighting dimmer switch includes a charging module used for providing 12V direct current, wherein the charging module is connected to a charging interface of a light emitting diode (LED) lamp. The decorative lighting dimmer switch further includes a constant current source driving module connected to the charging module, wherein the constant current source driving module is connected to the LED lamp, and the constant current source driving module is a constant current source driving circuit including a PT4115 driving chip. The decorative lighting dimmer switch achieves a charging function and an on-line dimming function at a same time by arranging the constant current source driving module to drive the LED lamp to satisfy requirements of consumers.

Abstract: Devices for improved nanopore sensing are described. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. The structure can include an array of nanopore structures, each nanopore structure comprising a passage for fluid connection through the structure between the analyte reservoir and outlet chamber. Control terminals can be arranged for applying a control signal to alter the electrical potential difference across that nanopore structure. Some embodiments include an electronic circuit configured to detect a signal from an electrical transduction element at each nanopore structure. Additional structural features and methods of operating and making the devices are described.

Abstract: To sense the translocation of a molecule through a nanopore, there is directed to an inlet of the nanopore, having a nanopore fluidic resistance, RP, a molecule disposed in a cis fluidic ionic solution having a cis fluidic access resistance, RC. The molecule is caused to translocate through the nanopore from the inlet of the nanopore to an outlet of the nanopore and to a trans fluidic ionic solution having a trans fluidic access resistance, RT. The trans fluidic access resistance, RT, is of the same order of magnitude as the nanopore fluidic resistance, RP, and both RT and RP are at least an order of magnitude greater than the cis fluidic access resistance, RC. An indication of local electrical potential is produced at a site within the nanopore sensor that is on the trans fluidic ionic solution-side of the nanopore, to sense translocation of the molecule through the nanopore.

Abstract: The invention relates to a process for producing a substrate comprising an aperture, which process comprises providing a substrate which comprises a solid-state membrane and a chemical surface modification on a first surface of the solid-state membrane; and forming an aperture through the chemical surface modification and the solid-state membrane. The invention also relates to a substrate comprising a chemical surface membrane and an aperture, a sensor comprising such a substrate and an apparatus comprising such a substrate.

Abstract: There is disclosed a nanopore support structure comprising a wall layer comprising walls defining a plurality of wells, and overhangs extending from the walls across each of the wells, the overhang defining an aperture configured to support a membrane suitable for insertion of a nanopore. There is further disclosed a nanopore sensing device comprising a nanopore support structure, and methods of manufacturing the nanopore support structure and the nanopore sensing device.

Abstract: There is provided a nanopore sensor including cis and trans fluidic reservoirs. A nanopore is provided in a support structure separating the cis and trans reservoirs. The nanopore has an inlet in fluidic connection with the cis fluidic reservoir and an outlet in fluidic connection with the trans fluidic reservoir. The cis fluidic reservoir has a fluidic access resistance, RC, the trans fluidic reservoir has a fluidic access resistance, RT, and the nanopore has a fluidic resistance, RP. RP is of the same order of magnitude as RT and both RP and RT are at least an order of magnitude greater than RC. An electrical transduction element is disposed at a nanopore sensor site that exposes the transduction element to the trans reservoir. An electrical circuit is connected to the electrical transduction element for producing an electrical signal indicative of changes in electrical potential local to the trans reservoir.

Abstract: A file system executing on a computing device may store a placeholder for a file on secondary storage of the computing device. The placeholder may comprise a sparse data stream containing none or some of the data of the file and information which enables the remotely stored data of the file to be retrieved from the network. As some or all of the data for the file is being stored remotely, the computing device may rely on a storage virtualization provider to create metadata for the file. Thus, the file system executing on the computing device may receive, from the storage virtualization provider, a request to store metadata associated with the file. In response to this request, the file system may store the metadata as a Binary Large Object (BLOB) in a secondary data stream of the placeholder for the file.

Abstract: Using metadata for a contentless file to provide a guest context access to file content. Within a guest context, a file system is mounted from a container image which lacks a first file's content and which includes metadata defining properties of the first file and mapping data defining information for identifying a second file within another filesystem from which the first file's content is obtainable Based on the properties, a filesystem operation involving the first file is performed without switching to a host context, and a requested access to the first file's content is responded to. Responding includes, based on the mapping data, communicating a request for the host context to supply the first file's content and, after returning from a context switch, responding to the requested access by supplying content of the second file from guest memory page(s) which are mapped to host memory page(s) containing the second file's content.

Abstract: Methods, systems, and computer storage media for providing a set of common flat files in a composite image that can be mounted as a container (i.e. composite container) to support isolation and interoperation of computing resources. Container management is provided for a container management system based on a composite image file system engine that executes composite operations. In particular, a remote sharing manager operates with a composite engine interface to support generating composite images configured for split layer memory sharing, split layer direct access memory sharing, and dynamic base images. In operation, a plurality of files and a selection of a remote sharing configuration for generating a composite image are accessed. The composite image for the plurality of files and the remoting sharing configuration is generated. The composite image is communicated to cause sharing of the composite image, sharing of the composite image is based on the remote sharing configuration.

Abstract: Methods, systems, and computer storage media for providing a set of common flat files in a composite image that can be mounted as a container (i.e., composite container) to support isolation and interoperation of computing resources. Container management is provided for a container management system based on a composite image file system engine that executes composite operations to support resource isolation and operating system (OS) virtualization functionality. In particular, a container engine interface supports creating, mounting, and providing access to and from composite images or composite containers. In operation, a plurality of files for generating a composite image are accessed. The composite image for the plurality of files is generated. The composite image includes a set of common flat files. The composite image is communicated to cause mounting of the composite image, where mounting the composite image is based on a metadata file from the set of common flat files.