Abstract: The present disclosure relates to the field of bioengineering technology, and particularly to an electrical signal communication platform for mimicking cardiomyocyte clusters. The transmitting end employs an Arduino Mega 2560™ main control board and an L298N motor drive module, the main control board sends a pulse signal to the motor drive module, and outputs a pulse waveform to a communication channel through the motor drive module; the communication channel employs a plurality of cell clusters, configured for receiving the pulse waveform output by the motor drive module and transmitting it to the receiving end; and the receiving end employs an oscilloscope to receive the pulse waveform passing through the communication channel, to demodulate the pulse waveform and send a demodulation result to a display device. The present disclosure provides multi-angle data acquisition to enhance the comprehensiveness of analysis, and facilitates a more accurate simulation of cardiomyocyte pacing and communication.

Abstract: A voice interaction method includes, when detecting a first voice command of a first user, first determining a screen corresponding to the first user, for example, a first screen, and then determining the first screen as a primary voice screen. The voice interaction method further includes displaying a first voice interaction interface through the first screen. In a process in which a vehicle-mounted device receives the first voice command, when detecting a second voice command issued by a second user, and determining a screen corresponding to the second user as another screen, determining the second screen as a secondary voice screen, displaying a second voice interaction interface through the second screen, and displaying, through the first screen, an interaction identifier indicating that there is another interacting user.

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: An optical perfect matching solver based on frequency grouping and multi-photon coincidence measurement comprises a broadband biphoton source, a wavelength selector, and a multi-photon coincidence detection system. The broadband biphoton source generates photon pairs using pulsed laser pumping in a nonlinear material with broadband phase matching. The wavelength selector, made up of a grating and a spatial light modulator, can combine any frequency component of the input light into a designated output port. The multi-photon coincidence detection system comprises single-photon detection channels and a coincidence counting logic circuit board, is used to measure multi-photon coincidence counts and distributions.

Abstract: The present invention provides a method for constructing an L-valine production strain, the L-valine production strain, and use thereof. According to the method for constructing the L-valine-producing strain, a 2,3-butanediol- or acetoin-producing strain is used as a starting strain, and genetic engineering modification is performed on the strain to improve the L-valine yield thereof. The present invention provides a new thought and way for efficient production of L-valine, and obtains a new production strain for efficiently producing L-valine. The L-valine-producing strain obtained in the present invention requires a simple culture medium and has low fermentation substrate and culture costs; meanwhile, the strain has a high L-valine yield and has a single product component easy to separate.

Abstract: Provides an artificial enzyme obtained by improving upon a sequence of a natural nicotine dehydrogenase, wherein the improvement comprises replacing at least one amino acid hindering product release with an amino acid with smaller side chains, thereby improving a catalytic rate.

Abstract: A method for preparing heat-conductive cement slurry for well cementation includes the following steps: S1, uniformly mixing sodium 1-butanesulfonate, sodium dodecyl diphenyl ether disulfonate and polyvinylpyrrolidone to obtain an admixture; S2, dissolving the admixture in deionized water and stirring to obtain a dispersant solution; S3, adding graphite to the dispersant solution and stirring to obtain a graphite dispersion; S4, stirring cement and deionized water in a slurry cup to obtain cement slurry; and S5, mixing and stirring the graphite dispersion and the cement slurry to obtain the heat-conductive cement slurry. The heat-conductive cement slurry can effectively improve the heat conductivity coefficient of set cement, and significantly improve the heat conductivity of the set cement, and has a broad market application prospect.

Abstract: A point-of-interest (POI) information management method includes: obtaining a webpage link; obtaining a webpage text associated with the webpage link; obtaining at least one item of POI information matching the webpage text from a POI information repository; presenting the at least one item of the POI information; in response to a pre-determined operation being performed on a first item of the POI information of the at least one item of the POI information, presenting a route from a current location to an address indicated in the first item of the POI information and a first operable icon; and in response to detecting another pre-determined operation is performed on the first operable icon, setting the route as a target trip.

Abstract: The present invention discloses an L-alanine-producing genetically engineered strain, as well as a method of construction and use thereof, and pertains to the field of bioengineering. According to the present invention, through enhancing the glycolysis pathway or/and introducing a gene for thermostable alanine dehydrogenase, a genetically engineered strain capable of high-yield production of alanine under a high temperature condition of 42° C. to 55° C. can be constructed. Moreover, through knocking out alanine racemase genes, optical purity of L-alanine can be significantly increased. When the original strain possesses a lactate synthesis pathway, blocking this lactate synthesis pathway can augment the proportion of a pyruvate synthesis pathway, resulting in an additionally increased yield of L-alanine.

Abstract: Provided are a method for constructing a lactic acid-producing strain, a lactic acid-producing strain and use thereof. The method for constructing lactic acid-producing strains is characterized by genetically engineering a starting strain to increase lactic acid production, wherein the engineering includes: 1) introducing a lactic acid synthesis pathway; 2) optimizing the lactic acid synthesis pathway; and 3) inhibiting by-product synthesis pathways.

Abstract: A transcriptional regulator LhgR is specifically responsive to L-2-hydroxyglutarate (L-2-HG) and an L-2-HG biosensor based on this transcriptional regulator; wherein the biosensor is a fusion protein of cyan fluorescent protein mTFP, L-2-HG specific transcriptional regulator LhgR, and yellow fluorescent protein Venus, including three types of L-2-HG biosensor LHGFR0N0C, LHGFR0N3C, and LHGFR0N7C. The application of the L-2-HG biosensor in the detection of L-2-HG-containing biological samples, real-time detection of intracellular L-2-HG concentration in bacteria and in human cells. The experiments confirmed that the biosensor can achieve high specificity, sensitivity, and accuracy in the detection of L-2-HG and can determine intracellular L-2-HG dynamics in real time, which has good application prospects.

Abstract: The present invention discloses a DL-alanine-producing genetically engineered strain, as well as a method of construction and use thereof, and pertains to the field of bioengineering. According to the present invention, through enhancing the glycolysis pathway or/and introducing thermostable alanine dehydrogenase, a genetically engineered strain capable of high-yield production of alanine at 42° C. to 55° C. This strain can be used in a two-step method for producing racemic DL-alanine, which includes fermentation and subsequent addition of microbial alanine racemase. Through inactivating or deleting alanine racemase genes in this strain and then separately introducing overexpressed alanine racemase gene(s), a genetically engineered strain capable of producing racemic DL-alanine using a direct fermentation method can be constructed.

Abstract: The present disclosure provides a proteolysis-targeting compound TPB-L-E3B, a method for synthesizing the same, and use thereof. The compound can treat human tumor diseases through the eRF3a-targeting proteolysis mechanism, and exhibits great potential in treating such diseases in in-vitro studies, particularly, in treating diseases such as prostate cancer, ovarian cancer, liver cancer, cervical cancer, leukemia, breast cancer, and the like.

Abstract: A motor vehicle lock comprising a locking mechanism which essentially consists of a rotary latch and a pawl, further comprising a drive and a securing element. The drive acts on the securing element via a toothed rack element that is equipped with a blocking element. According to the invention, the blocking element is in the form of a tilting lever defining at least two end positions of the toothed rack element.

Abstract: The disclosure provides a construction method including steps of: constructing a detailed model and a simplified model of the rail vehicle using finite element method based on the three-dimensional structure of the rail vehicle; initially setting the simplified model in accordance with the detailed model, including specifying materials, mechanical properties parameters, and mass point; setting different simplification thickness ratio to obtain a plurality of sets of the simplified models, designing longitudinal and lateral collision conditions, and in each type of the collision conditions, performing collision simulations on the detailed model and each set of the simplified models under each thickness ratio to obtain respective collision response curves; calculating a total CORA value corresponding to the plurality of sets of the simplified models; selecting an optimal simplified model from n sets of the simplified models with the highest total CORA values.

Abstract: Provided are a vehicle monitoring method and a vehicle monitoring system. The vehicle monitoring method includes that: a polarization angle of polarized light in a sky image reflected by a vehicle window in a monitoring scenario is calculated, and a light-filtering polarization angle is calculated according to the polarization angle of the polarized light in the sky image reflected by the vehicle window, where the polarized light in the sky image is formed by scattered sunlight in a sky region corresponding to the sky image; the polarized light in the sky image reflected by the vehicle window in the monitoring scenario is filtered out according to the light-filtering polarization angle; and the monitoring scenario is imaged to form a monitoring image.

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: Provided are a genetically engineered strain for producing polylactic acid and a method for producing polylactic acid. The genome of the genetically engineered strain is integrated with a coding sequence of exogenous D-lactate dehydrogenase gene, a coding sequence of exogenous propionyl-CoA transferase gene, and a coding sequence of exogenous polyhydroxyalkanoate synthase gene, enabling the genetically engineered strain to express exogenous D-lactate dehydrogenase, exogenous propionyl-CoA transferase, and exogenous polyhydroxyalkanoate synthase. The method includes: providing the above genetically engineered strain of Synechococcus elongatus; introducing carbon dioxide and culturing the genetically engineered strain under light; and when a growth OD of the genetically engineered strain reaches the maximum, collecting and drying the genetically engineered strain, and recycling the polylactic acid in the strain.

Abstract: A double-channel fluid injection apparatus includes a main shaft, a bearing box, and a sealing box. The main shaft includes an outer tube and an inner tube. An internal channel of the inner tube and a vertical through channel constitute a first fluid passageway. The inner tube and the outer tube form an annular gap, and a channel D is radially formed at a lower part of the outer tube and is in communication with the annular gap, thus constituting a second fluid passageway. The bearing box is mounted on a boss of the outer tube. The sealing box includes a sealing cylinder fixedly connected to a lower end of a lower end cap of the bearing box. A channel E in communication with the second fluid passageway is radially formed at a position of the sealing cylinder corresponding to the channel of the outer tube.

Abstract: A startup stage protection device used in Electric Multiple Unit (EMU) train coupler experiment is provided between two test cars. The startup stage protection device is arranged between and tightly abuts two test cars at the starting stage of the experiment to receive a compressing force in place of the coupler. The startup stage protection device separates from the two test cars after the end of the starting stage.