Patents by Inventor Prasanna Thwar
Prasanna Thwar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230417787Abstract: The present disclosure provides for processes and systems that rely on software, bioinformatics, and robotics principles for actuating the automation of a workflow from nucleic acid extraction from a sample to sequencing without human intervention in between the aforementioned steps.Type: ApplicationFiled: June 23, 2023Publication date: December 28, 2023Inventors: Tiffany Cheung, Michael Balamotis, Werther Dong, Alfred Haddad, Prasanna Thwar
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Publication number: 20230417786Abstract: The present disclosure provides for processes and systems that rely on software, bioinformatics, and robotics principles for actuating the automation of a workflow from nucleic acid extraction from a sample to sequencing without human intervention in between the aforementioned steps.Type: ApplicationFiled: June 23, 2023Publication date: December 28, 2023Inventors: Tiffany Cheung, Michael Balamotis, Werther Dong, Alfred Haddad, Prasanna Thwar
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Publication number: 20220154270Abstract: Provided herein are fully-automated next-generation sequencing platforms and processes for detection of a target specimen (e.g., SARS-CoV-2) and for distinguishing infectious from non-infectious signals from the specimen. An analysis can provide simultaneous diagnosis and genomic surveillance of a multitude of distinct specimens in a sample information. The analysis can comprise distinguishing between infectious versus infectious specimens and provide a recommendation as to how infectious the sample can be. The information on can be used to better inform the status of a subject or a location with regards to infectivity from the specimen.Type: ApplicationFiled: November 19, 2021Publication date: May 19, 2022Inventors: Sasan Amini, Ramin Khaksar, Michael Taylor, Prasanna Thwar Krishnan, David Tran, Shaokang Zhang, Shadi Shokralla, Kyle Rhoden, Susan Jisoon Lee, Bryan Thomas Barney, Sima Mortazavi, Adam Forrest Allred, Henrik Gehrmann
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Publication number: 20220084630Abstract: Food processing facilities should employ environmental sampling programs to monitor for general levels of hygiene (the efficacy of general cleaning and sanitation for the removal of transient microorganisms). The instant disclosure provides kits, systems and methods for amplifying a portion of a genome of a pathogen at a plurality of physical locations within a facility; and associating, via a computer, the presence of said pathogen with a location of the plurality of physical locations within said facility.Type: ApplicationFiled: November 23, 2021Publication date: March 17, 2022Inventors: Sasan AMINI, Ramin KHAKSAR, Michael TAYLOR, Julius Christopher BARSI, Hossein NAMAZI, Adam ALLRED, Shaokang ZHANG, Henrik GEHRMANN, Kyle S. RHODEN, Shadi SHOKRALLA, Daniel MCDONOUGH, Prasanna Thwar KRISHNAN
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Patent number: 11248265Abstract: Provided herein are fully-automated next-generation sequencing platforms and processes for detection of a target specimen (e.g., SARS-CoV-2) and for distinguishing infectious from non-infectious signals from the specimen. An analysis can provide simultaneous diagnosis and genomic surveillance of a multitude of distinct specimens in a sample information. The analysis can comprise distinguishing between infectious versus infectious specimens and provide a recommendation as to how infectious the sample can be. The information can be used to better inform the status of a subject or a location with regards to infectivity from the specimen.Type: GrantFiled: November 23, 2020Date of Patent: February 15, 2022Assignee: CLEAR LABS, INCInventors: Sasan Amini, Ramin Khaksar, Michael Taylor, Prasanna Thwar Krishnan, David Tran, Shaokang Zhang, Shadi Shokralla, Kyle Rhoden, Susan Jisoon Lee, Bryan Thomas Barney, Sima Mortazavi, Adam Forrest Allred, Henrik Gehrmann
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Patent number: 10941439Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: GrantFiled: October 31, 2018Date of Patent: March 9, 2021Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Steven M. Menchen, Guobing Xiang, Prasanna Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
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Publication number: 20190062807Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.Type: ApplicationFiled: October 31, 2018Publication date: February 28, 2019Inventors: Steven M. Menchen, Guobing Xiang, Prasanna Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
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Patent number: 9901887Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: GrantFiled: September 30, 2016Date of Patent: February 27, 2018Assignee: Life Technologies CorporationInventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech, John Andrew Sheridan
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Patent number: 9861986Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: GrantFiled: October 3, 2014Date of Patent: January 9, 2018Assignee: Advanced Liquid Logic, Inc.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Vijay Srinivasan, Allen E. Eckhardt, Alexander Shenderov, Dwayne J. Allen
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Publication number: 20170266653Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.Type: ApplicationFiled: June 6, 2017Publication date: September 21, 2017Inventors: Michael G. POLLACK, Ramakrishna SISTA, Zhishan HUA, Vamsee K. PAMULA, Arjun SUDARSAN, Vijay SRINIVASAN, Prasanna THWAR, Alexander SHENDEROV, Dwayne ALLEN
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Patent number: 9675972Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.Type: GrantFiled: March 9, 2009Date of Patent: June 13, 2017Assignee: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Ramakrishna Sista, Zhishan Hua, Vamsee K. Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar, Alexander Shenderov, Dwayne Allen
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Publication number: 20170014784Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: ApplicationFiled: September 30, 2016Publication date: January 19, 2017Inventors: Jonathan SCHULTZ, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech, John Andrew Sheridan
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Publication number: 20160271623Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.Type: ApplicationFiled: May 26, 2016Publication date: September 22, 2016Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Vamsee K. Pamula, Prasanna Thwar, Arjun Sudarsan, Ramakrishna Sista
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Patent number: 9358551Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.Type: GrantFiled: October 30, 2015Date of Patent: June 7, 2016Assignee: ADVANCED LIQUID LOGIC, INC.Inventors: Prasanna Thwar, Vamsee K. Pamula, Arjun Sudarsan, Ramakrishna Sista
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Publication number: 20160051992Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.Type: ApplicationFiled: October 30, 2015Publication date: February 25, 2016Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: PRASANNA THWAR, VAMSEE K. PAMULA, ARJUN SUDARSAN, RAMAKRISHNA SISTA
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Patent number: 9205433Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.Type: GrantFiled: April 27, 2015Date of Patent: December 8, 2015Assignee: Advanced Liquid Logic, Inc.Inventors: Prasanna Thwar, Vamsee K. Pamula, Arjun Sudarsan, Ramakrishna Sista
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Patent number: 9121047Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: GrantFiled: February 25, 2013Date of Patent: September 1, 2015Assignee: Life Technologies CorporationInventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech, Andrew Sheridan
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Patent number: 9017993Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: GrantFiled: April 9, 2012Date of Patent: April 28, 2015Assignee: Life Technologies CorporationInventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech
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Publication number: 20150107995Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads.Type: ApplicationFiled: January 5, 2015Publication date: April 23, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Ramakrishna Sista, Vamsee Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar
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Publication number: 20150038344Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.Type: ApplicationFiled: October 22, 2014Publication date: February 5, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Allen E. Eckhardt, Prasanna Thwar, Jeremy Rouse