Patents by Inventor Prasanna Thwar
Prasanna Thwar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150027889Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: ApplicationFiled: October 3, 2014Publication date: January 29, 2015Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Vijay Srinivasan, Allen E. Eckhardt, Alexander Shenderov, Dwayne J. Allen
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Patent number: 8927296Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads.Type: GrantFiled: May 18, 2009Date of Patent: January 6, 2015Assignee: Advanced Liquid Logic, Inc.Inventors: Ramakrishna Sista, Vamsee Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar
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Patent number: 8852952Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: GrantFiled: May 4, 2009Date of Patent: October 7, 2014Assignee: Advanced Liquid Logic, Inc.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Allen Eckhardt, Alexander Shenderov, Dwayne Allen, Vijay Srinivasan
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Publication number: 20140174933Abstract: A droplet actuator comprising: (a) a base substrate comprising electrodes configured for conducting droplet operations on a droplet operations surface thereof; (b) a droplet comprising one or more beads situated on the droplet operations surface; (c) a barrier arranged in relation to the droplet and the electrodes such that a droplet may be transported away from the beads using one or more droplet operations mediated by one or more of the electrodes while transport of the beads is restrained by a barrier.Type: ApplicationFiled: November 25, 2013Publication date: June 26, 2014Applicant: Advanced Liquid Logic, Inc.Inventors: ARJUN SUDARSAN, MICHAEL G. POLLACK, PRASANNA THWAR, DWAYNE ALLEN
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Publication number: 20140057363Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads.Type: ApplicationFiled: May 18, 2009Publication date: February 27, 2014Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Ramakrishna Sista, Vamsee Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar
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Patent number: 8658111Abstract: The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.Type: GrantFiled: February 22, 2011Date of Patent: February 25, 2014Assignee: Advanced Liquid Logic, Inc.Inventors: Vijay Srinivasan, Vamsee Pamula, Ramakrishna Sista, Arjun Sudarsan, Prasanna Thwar
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Patent number: 8591830Abstract: A droplet actuator comprising: (a) a base substrate comprising electrodes configured for conducting droplet operations on a droplet operations surface thereof; (b) a droplet comprising one or more beads situated on the droplet operations surface; (c) a barrier arranged in relation to the droplet and the electrodes such that a droplet may be transported away from the beads using one or more droplet operations mediated by one or more of the electrodes while transport of the beads is restrained by a barrier. Related methods and kits are also provided.Type: GrantFiled: August 25, 2008Date of Patent: November 26, 2013Assignee: Advanced Liquid Logic, Inc.Inventors: Arjun Sudarsan, Michael G. Pollack, Prasanna Thwar, Dwayne Allen
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Publication number: 20130288254Abstract: The invention is directed to certain droplet actuated molecular techniques. In one embodiment, the invention provides droplet actuator methods for detection of single nucleotide polymorphisms (SNPs) in a DNA sequence using digital microfluidics, including droplet actuator-based sample preparation and SNP analysis. In another embodiment, the invention provides droplet actuator devices and methods for providing integrated sample preparation and multiplexed detection of an infectious agent, such as HIV. In yet another embodiment, the invention provides droplet actuator devices and techniques for PCR amplification and detection of specific nucleic acid sequences using digital microfluidics, including droplet actuator-based sample preparation and target nucleic acid analysis. In yet another embodiment the invention provides methods for performing hot-start PCR on a droplet actuator.Type: ApplicationFiled: August 13, 2010Publication date: October 31, 2013Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Vijay Srinivasan, Allen E. Eckhardt, Prasanna Thwar, Zhishan Hua, Arjun Sudarsan, Theodore Winger
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Publication number: 20130233425Abstract: The present invention is directed to modified droplet actuators, fluids and methods for enhancing and/or maintaining oil film stability in a droplet actuator. In an exemplary embodiment, the invention provides a droplet actuator with one or more substrates arranged to form a droplet operations gap comprising gap-facing surfaces; droplet operations electrodes configured to conduct droplet operations in the droplet operations gap; at least one barrier included on at least one of the substrate surfaces and having dimensions selected to: permit droplet transport from atop a first droplet operations electrode to a second droplet operations electrode when the second droplet operations electrode is activated; and prevent movement of a droplet from atop a first droplet operations electrode when the first and second droplet operations electrodes are inactive.Type: ApplicationFiled: April 19, 2013Publication date: September 12, 2013Applicant: ADVANCED LIQUID LOGIC INC.Inventors: Vijay Srinivasan, Vamsee K. Pamula, Ramakrishna Sista, Prasanna Thwar
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Publication number: 20130225450Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.Type: ApplicationFiled: August 28, 2012Publication date: August 29, 2013Applicant: Advanced Liquid Logic IncInventors: Michael G. Pollack, Allen E. Eckhardt, Prasanna Thwar, Jeremy Rouse
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Publication number: 20130225452Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.Type: ApplicationFiled: August 28, 2012Publication date: August 29, 2013Applicant: ADVANCED LIQUID LOGIC INCInventors: Michael G. Pollack, Allen E. Eckhardt, Prasanna Thwar, Jeremy Rouse
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Publication number: 20130203606Abstract: A method of preparing a nucleic acid library in droplets in contact with oil, including: (a) blunt-ending nucleic acid fragments in a droplet in the oil to yield blunt-ended nucleic acid fragments; (b) phosphorylating the blunt-ended nucleic acid fragments in a droplet in the oil to yield phosphorylated nucleic acid fragments; coupling A-tails to the phosphorylated nucleic acid fragments in a droplet in the oil to yield A-tailed nucleic acid fragments; and (d) coupling nucleic acid adapters to the A-tailed nucleic acid fragments in a droplet in the oil to yield the nucleic acid library comprising adapter-ligated nucleic acid fragments.Type: ApplicationFiled: February 22, 2011Publication date: August 8, 2013Applicant: ADVANCED LIQUID LOGIC INCInventors: Michael G Pollack, Allen E. Eckhardt, Prasanna Thwar, Jeremy Rouse
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Publication number: 20120258516Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.Type: ApplicationFiled: April 9, 2012Publication date: October 11, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Thwar, Todd Roswech
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Publication number: 20110311980Abstract: The invention provides a droplet actuator device, as well as systems, methods and devices making use of the droplet actuator device. The droplet actuator device may include a substrate having electrodes arranged for conducting one or more droplet operations. The droplet actuator device may include a substrate having a reactor path with a wash region associated with a magnet for immobilizing mobilizing beads during bead washing operations. The droplet actuator device may include nucleotide base reservoirs and dedicated nucleotide base electrode paths arranged for transporting nucleotide base droplets from nucleotide base reservoirs to the reactor path. The droplet actuator device may include one or more wash buffer reservoirs associated with electrode paths arranged for transporting wash buffer droplets from wash buffer reservoirs to the reactor path.Type: ApplicationFiled: December 15, 2009Publication date: December 22, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Allen E. Eckhardt, Vijay Srinivasan, Prasanna Thwar
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Publication number: 20110256542Abstract: The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.Type: ApplicationFiled: August 24, 2009Publication date: October 20, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Zhishan Hua, Allen E. Eckhardt, Prasanna Thwar, Vijay Srinivasan, Vamsee K. Pamula
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Publication number: 20110180571Abstract: The present invention provides droplet actuators, modified fluids and methods relating to droplet operations. An aspect includes a droplet actuator including a droplet operations substrate; an oil based filler fluid on the droplet operations substrate comprising an oil soluble additive in the filler fluid; and a droplet in contact with the oil based filler fluid. Still other aspects are provided.Type: ApplicationFiled: February 22, 2011Publication date: July 28, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Vijay Srinivasan, Vamsee Pamula, Ramakrishna Sista, Arjun Sudarsan, Prasanna Thwar
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Publication number: 20110104816Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.Type: ApplicationFiled: May 4, 2009Publication date: May 5, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Prasanna Thwar, Vamsee K. Pamula, Vijay Srinivasan, Allen Eckhardt, Alexander Shenderov, Dwayne Allen
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Publication number: 20110104747Abstract: Methods of concentrating beads in a droplet and/or loading beads on a fluidic device are provided, including among other things, a method of concentrating beads in a droplet, the method comprising: (a) providing a droplet actuator comprising: (i) an interior droplet operations volume; and (ii) a reservoir exterior to the interior volume; (iii) a droplet established in a liquid path extending from the reservoir into the interior volume; (b) providing magnetically responsive beads in the portion of the droplet which is in the reservoir; (c) magnetically attracting the magnetically responsive beads through the liquid path into the portion of the droplet which is in the interior volume; and (d) forming a droplet comprising one or more of the magnetically responsive beads in the interior volume.Type: ApplicationFiled: March 9, 2009Publication date: May 5, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Ramakrishna Sista, Zhishan Hua, Vamsee K. Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar, Alexander Shenderov, Dwayne Allen
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Publication number: 20110097763Abstract: The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.Type: ApplicationFiled: May 13, 2009Publication date: April 28, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Michael G. Pollack, Zhishan Hua, Allen E. Eckhardt, Prasanna Thwar, Vijay Srinivasan, Vamsee K. Pamula
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Publication number: 20110091989Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads.Type: ApplicationFiled: May 18, 2009Publication date: April 21, 2011Applicant: ADVANCED LIQUID LOGIC, INC.Inventors: Ramakrishna Sista, Vamsee Pamula, Arjun Sudarsan, Vijay Srinivasan, Prasanna Thwar