Abstract: Provided are compositions and methods for precise genome editing. The compositions include a fusion protein comprising a T4 DNA polymerase segment and a segment of an MS2 bacteriophage coat protein. The fusion protein operates with a Cas enzyme and one or more guide RNAs to produce one or more indels. The indel is produced in a DNA repair template free manner. Methods for producing the indels are also provided. A method includes introducing into the cell a fusion protein containing a T4 DNA polymerase segment and a segment of an MS2 bacteriophage coat protein, a Cas enzyme, and a guide RNA comprising MS2 protein binding sites. The guide RNA directs the Cas enzyme, the T4 DNA polymerase and the MS2 binding protein to the selected chromosome locus to produce the indel. The indel may correct a mutation in an open reading frame encoded by the selected chromosome locus.

Abstract: Provided are compositions and methods that include an engineered DNA polymerase used in combination with a Cas9 protein. The combination exhibits improved on-target chromosomal alterations, increases the proportion of precise 1- to 3-base-pair insertions at target sites, and reduces translocations caused by previously available systems.

Abstract: A bed system with multiple functions includes a frame structure; a plurality of platforms disposed on the frame structure; an adjustable assembly coupled with the frame structure and the plurality of platforms for operably adjusting one or more of the plurality of platforms in desired positions; a plurality of fans mounted onto openings in the plurality of platforms and adapted for operably providing air circulation in a surrounding space of the bed system, and an aromatherapy system attached onto the one or more platforms for producing desired fragrance in the surrounding space so as to promote health and well-being of a user. Each aromatherapy device is configured to produce a fragrance when said aromatherapy device is turned on, and one or more working modes. The fragrance is identical to or different from that produced by other aromatherapy device of the one or more aromatherapy devices.

Abstract: A mattress includes a plurality of layers vertically stacked to one another, the plurality of layers comprising a first layer; a second layer; and a third layer. The first layer comprises a plurality of openings defined therein being operably in fluidic communication with a plurality of fans for providing air circulation to a user through the mattress. The second layer is disposed between the first layer and the third layer and comprises an array of springs.

Abstract: An adjustable bed system includes a frame structure, a back lifting assembly and a leg lifting assembly pivotally connected to the frame structure, platforms having a plurality of openings, disposed on the frame structure and operably coupled with the back and leg lifting assemblies, a mattress having a plurality of through holes and disposed on the platforms so that the plurality of through holes is aligned with the plurality of openings, respectively, and a plurality of fans installed in the plurality of openings of the platforms such that each fan is in communication with a respective through hole of the mattress for operably moving air via said through hole into the mattress body and outputting the air from the top surface of the mattress. Positions of upper and lower portions of the mattress are adjustable based on operations of the back and assemblies.