Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.

Abstract: The present invention features a method for isolating and purifying proteins and peptides of interest from a plant host, which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating proteins and peptides of interest than those methods described in the prior art.

Abstract: A flexible automated apparatus for isolating and purifying viruses, proteins and peptides of interest from a plant material is disclosed, the apparatus being applicable for large scale purification and isolation of such substances from plant material. The flexible automated apparatus provides an efficient apparatus for isolating viruses, proteins and peptides of interest with little waste material. The automated apparatus for isolating viruses, proteins and peptides of interest includes a grinding apparatus for homogenizing a plant to produce a green juice, a means for adjusting the pH of and heating the green juice, a means for separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.

Abstract: The present invention features a method for isolating and purifying vitamins and sugars from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating vitamins and sugars than those methods described in the prior art. In general, the present method of isolating vitamins and sugars comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either vitamins or sugars, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying vitamins or sugars by such procedure as PEG-precipitation, chromatography and/or salt precipitation.

Abstract: A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.

Abstract: The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspenion, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Abstract: The present invention features a method for isolating and purifying viruses, proteins and peptides of interest from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating viruses, proteins and peptides of interest than those methods described in the prior art. In general, the present method of isolating viruses, proteins and peptides of interest comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either virus or protein/peptide, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying virus particles by such procedure as PEG-precipitation or purifying proteins and peptides by such procedures as chromatography and/or salt precipitation.

Abstract: The present invention relates to the production of three (3) distinct savory flavor types of dehydrated mushrooms: (1) a light-colored material with strong mushroom flavor, (2) a tan-colored material with a meaty, buttery and savory flavor and (3) a dark-colored material with a beefy, meaty, chocolate flavor. All three products exhibit "umami" or monosodium glutamate (MSG)-like mouthfeel and flavor synergy. The mushrooms are produced using specialized nutrient additives, controlled growing conditions and specialized dehydration protocols.

Abstract: The present invention relates to the production of three (3) distinct savory flavor types of dehydrated mushrooms: (1) a light-colored material with strong mushroom flavor, (2) a tan-colored material with a meaty, buttery and savory flavor and (3) a dark-colored material with a beefy, meaty, chocolate flavor. All three products exhibit "umami" or monosodium glutamate (MSG)-like mouthfeel and flavor synergy. The mushrooms are produced using specialized nutrient additives, controlled growing conditions and specialized dehydration protocols.

Abstract: The present invention includes an improved nutrient, in particle form, for use in enhancing fungus growth, and particularly directed to edible mushrooms. The nutrient is in the form of conglomerate particles including an irregularly shaped matrix particle and a plurality of enhancer droplets attached to the matrix particle at various recessed attachment positions. The matrix material is denatured protein while the enhancer droplets are fatty oil and phospholipid material microencapsulated in a layer of activated protein mixed with mycelium growth stimulators. The present invention further includes a method for preparing nutrient particles for increasing the growth and yield of mushrooms.

Abstract: The present invention is a process for preparing growth and nutritional enhancement additives for use with mushroom cultivation and products prepared therefrom. The process includes subprocesses for preparing an activator particle containing ingredients which help maximize growth activity in the fungus and a combination particle which includes activator particles and delayed release nutrient material which helps to sustain optimal growth. The particles produced by the process, namely the activator particle, the combination particle and a method for bringing the particles into contact with mushroom mycelia at the optimum time are also included. The invention is intended to maximize efficient, rapid and healthy growth in commercial mushroom strains.

Abstract: The present invention includes an improved nutrient, in particle form, for use in enhancing fungus growth, and particularly directed to edible mushrooms. The nutrient is in the form of conglomerate particles including an irregularly shaped matrix particle and a plurality of enhancer droplets attached to the matrix particle at various recessed attachment positions. The matrix material is denatured protein while the enhance droplets are fatty oil and phospholipid material microencapsulated in a layer of activated protein mixed with mycelium growth stimulators. The present invention further includes a method for preparing nutrient particles for increasing the growth and yield of mushrooms.