Abstract: The present invention provides a method of making a genetically transformed plant which has an altered content of at least one product of a secondary metabolic pathway. The method consists of introducing into a plant cell capable of being transformed and regenerated to a whole plant a DNA expression cassette. The expression cassette includes DNA sequences required for transformation and selection in plant cells. It also includes a DNA sequence that, under the control of a promoter active in plant cells, encodes a protein capable of modifying the utilization of a substrate in the secondary metabolic pathway. The substrate is not a primary metabolite of the group selected from glucose, amino acids, common fatty acids and nucleotides. A plant or plant tissues including seeds can then be recovered having an altered content of at least one product of the secondary metabolic pathway. The invention also provides for feed products derived from the plants and seeds obtained according to the method.

Abstract: The sinapine content of seeds of Brassica napus, and other crucifera plants, and the resulting seed meal made therefrom, is reduced by transforming cells of the plants to incorporate an expressible exogenous CYP84 monooxygenase enzyme, particularly ferulate 5-hyroxylase (F5H:) enzyme, or an antisense equivalent thereof. This allows for the production of a seed meal that is commercially more valuable. Three specific nucleic acid sequences encoding the F5H polypeptide are disclosed, designated BNF5H1, BNF5H2 and BNF5H3, and genetic constructs produced. The antisense suppression of sinapine is preferred, which can reduce the sinapine content of seed meal by up to 40% compared to wild type or vector-only transformed plants.

Abstract: Probes and methods of using the probes to detect chromosomal rearrangements and/or deletions are provided. The methods utilize probes that are free of repeat sequences to provide greater selectivity and sensitivity; methods for producing such probes are also disclosed. The probe sets utilized in the detection methods are designed to hybridize to chromosomes at regions outside known breakpoints, instead of spanning the breakpoint as with conventional FISH methods, and, in some instances, are further designed to bind to regions located outside the genes involved in the rearrangement. Methods utilizing probe sets with two and four colors are also described, as are automated methods for analyzing rearrangements.

Abstract: A lamp for recessed ceiling mounting consisting of a ceiling aperture lining flange defining an air dam receiving space; a mounting plate fixedly attached to and extending outwardly from the upper end of said flange; a ballast; spirally threaded screws removedly mounting the ballast upon the mounting plates; a concave air dam having an interior flourescent tube receiving space; a plurality of friction clips connecting the air dam to said flange, said clips positioning the air dam within said flange's air dam receiving space; a flourescent light tube; and a plurality of spring clips mounting the flourescent light tube within the interior space of the concave air dam.

Abstract: An animal, e.g., transgenic mouse, in which the MSH5 gene is misexpressed. The animal is useful for screening treatments for a number of conditions. Methods for identifying contraceptive agents are also described.

Abstract: This invention provides an isolated nucleic acid which encodes bcl-8. This invention also provides an oligonucleotide of at least 15 nucleotides capable of specifically hybridizing with a sequence of nucleotides within a nucleic acid which encodes bcl-8. Further, this invention provides an agent capable of blocking the expression of BCL-8. This invention also provides various methods of determining whether a subject is afflicted with diffuse large cell lymphoma. This invention further provides a method of determining whether a subject has a predisposition for diffuse large cell lymphoma. This invention further provides various methods of treating a subject afflicted with diffuse large cell lymphoma. This invention also provides various methods of preventing diffuse large cell lymphoma in a subject. This invention also provides pharmaceutical compositions for treating and/or preventing diffuse large cell lymphoma.

Abstract: A computer system and method for a lessor to determine correct use tax on moveable equipment which may be subject to tax by more than one tax authority comprising inventory records for each individual item of equipment comprising an equipment identifier, equipment category, acquisition cost, depreciation, net book value, and branch; updating the inventory records to reflect each modification, improvement, disposition, and change in equipment category, and use tax paid; activity records comprising each lease event pertaining to each item of equipment; and a set of tax rules, which are periodically updated, for each taxing authority where any of the items of equipment may be leased or used, comprising sets of formulas reflecting relationships between tax due and acquisition of equipment, first leasing of equipment, subsequent leasing of equipment, length of lease term, age of equipment, and/or equipment categories.

Abstract: This invention provides an isolated vertebrate nucleic acid molecule the bcl-6 locus. This invention also provides an isolated human nucleic acid molecule of bcl-6 locus. This invention further provides a nucleic acid molecule comprising a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with a sequence included within the sequence of the nucleic acid molecule of bcl-6 locus. This invention provides an isolated vertebrate nucleic acid molecule of bcl-6 operatively linked to a promoter of RNA transcription. This invention provides a vector which comprises the nucleic acid molecule of bcl-6 locus. This invention provides a host vector system for the production of a polypeptide encoded by bcl-6 locus, which comprises the vector of bcl-6 locus in a suitable host. This invention provides a polypeptide encoded by the isolated vertebrate nucleic acid molecule of bcl-6 locus. This invention provides an antibody capable of binding to polypeptide encoded by bcl-6 locus.

Abstract: This invention provides a method for detecting a rearrangement of a bcl-6 gene in B-cell lymphoma in a subject, comprising: (a) obtaining a DNA sample from the subject; (b) cleaving the DNA sample into fragments; (c) separating the DNA fragments by size fractionation; (d) hybridizing the DNA fragments with a nucleic acid molecule comprising at least 15 contiguous nucleotides which is complementary to a sequence of an isolated nucleic acid molecule having the nucleic acid sequence as set forth in SEQ ID NO:1 and specifically hybridizes with the nucleic acid sequence as set forth in SEQ ID NO:1 to detect the DNA fragment containing the bcl-6 nucleic acid sequence; and (e) comparing the detected DNA fragment from (d) with a DNA fragment from a known normal subject, the difference in size of the fragments indicating occurrence of a rearrangement of the bcl-6 gene in B-cell lymphoma in the subject.

Abstract: Salient embodiments comprise magnetic recording sliders whose record-confronting face is characterized by two or three rails wherein stiction forces are counter-acted by provision of "boss means" on one or several rails, or therebetween; and also characterized by provision of "back-bar means" and associated purge channel means; and methods for rendering such are also described.

Abstract: This invention provides an isolated vertebrate nucleic acid molecule the bcl-6 locus. This invention also provides an isolated human nucleic acid molecule of bcl-6 locus. This invention further provides a nucleic acid molecule comprising a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with a sequence included within the sequence of the nucleic acid molecule of bcl-6 locus. This invention provides an isolated vertebrate nucleic acid molecule of bcl-6 operatively linked to a promoter of RNA transcription. This invention provides a vector which comprises the nucleic acid molecule of bcl-6 locus. This invention provides a host vector system for the production of a polypeptide encoded by bcl-6 locus, which comprises the vector of bcl-6 locus in a suitable host. This invention provides a polypeptide encoded by the isolated vertebrate nucleic acid molecule of bcl-6 locus. This invention provides an antibody capable of binding to polypeptide encoded by bcl-6 locus.

Abstract: Genetic markers of this invention provide a biochemically assayable reporter activity and a conditionally selectable growth advantage. They comprise a first gene which encodes a product whose activity can be assdyed biochemically e.g., .beta.-glucuronidase, to a second gene which confers a conditional growth advantage such as antibiotic resistance in a transformed cell (e.g., neomycin phosphotransferase-II). The marker genes and their translational products are stable in cells. The genetic markers provide for the positive genetic selection and the biochemical and histochemical screening of transformed cells. They can be used as probes for identification and isolation of novel genetic regulatory elements, such as promoters, and the genes they regulate.

Abstract: Salient embodiments comprise magnetic recording sliders whose record-confronting face is characterized by two or three rails wherein stiction forces are counter-acted by provision of "boss means" on one or several rails, or therebetween; and also characterized by provision of "back-bar means" and associated purge channel means; and methods for rendering such are also described.

Abstract: Salient embodiments comprise a magnetic recording slider whose record-confronting face is characterized by two or three rails wherein stiction forces are counter-acted by provision of piezo-electric boss means on one or several rails, or therebetween.

Abstract: Salient embodiments comprise magnetic recording sliders whose record-confronting face is characterized by two or three rails wherein stiction forces are counter-acted by provision of "boss means" on one or several rails, or therebetween; and also characterized by provision of "back-bar means" and associated purge channel means; and methods for rendering such are also described.

Abstract: A disk drive having a hermetically sealed disk chamber capable of being filled with gas at pressures above ambient pressure with a specially designed connector assembly for use with the sealed chamber. Helium or nitrogen is injected into the sealed disk chamber. To decrease power consumption at high platter spin rates, the present invention advantageously utilizes a sealed disk chamber filled with helium to reduce tribological resistance forces associated with the surfaces of the spinning platters. Nitrogen is used to reduce oxidation of the disk platters. Special fixtures maintaining high position tolerance and connectors are designed to coupled the PC board controller of the disk drive unit to the components of the disk drive within the sealed disk chamber to eliminate connecting flexcircuits from the junction of the hermetically sealed chamber.

Abstract: Salient embodiments comprise magnetic recording sliders whose record-confronting face is characterized by two or three rails wherein stiction forces are counter-acted by provision of a "boss" on one or several rails, or therebetween; and also characterized by provision of a "back-bar" and associated a purge channel; and methods for rendering such are also described.

Abstract: A method of adapting a magnetic recording slider having a shallow "flying cavity" on its media-confronting face to exhibit "self-purging", better stability and increased stiffness, this method including: providing a back bar across the trailing face of the cavity, with a purge channel in the back-bar to purge the cavity of gas and associated contaminants.

Abstract: The present invention is directed to a method for producing high purity carbon monoxide. In the method, a hydrocarbon feed stock and import carbon dioxide are introduced into a reaction system containing a primary reformer and a secondary reformer. The hydrocarbon feed stock, import carbon monoxide and recycle carbon monoxide are passed through the primary reformer while being heated to a temperature sufficient to reform the feed stock into a first carbon monoxide reaction product. The first reaction product and oxygen are fed into the secondary reformer. The first reaction product is reacted in the secondary reformer in an autothermal reaction to provide a second carbon monoxide reaction product having a lower hydrocarbon level than the first reaction product. The second reaction product is removed from the secondary reformer at a second predetermined temperature and a high purity carbon monoxide is recovered from the second reaction product without the use of cryogenic separation techniques.

Abstract: Salient embodiments comprise a magnetic recording slider whose record-confronting face is characterized by two or three rails wherein stiction forces are counteracted by provision of piezo-electric boss means on one or several rails, or therebetween.