Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

Abstract: Bioreactor bags formed from sheet materials are disclosed. The bioreactor bags include an opening which is openable and recloseable in a fluid tight manner for providing an opening for insertion of solid materials into the bag.

Abstract: A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.

Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

Abstract: A bioreactor system and packaging is provided. The bioreactor system includes a vessel for housing biomaterials for processing and a support structure. The vessel includes a flexible material defining a chamber and a mixing system positioned within the chamber. The mixing system includes an agitator for imparting motion and mixing to the contents of the vessel and includes a base affixed to the flexible material at a base section of the chamber, a shaft moveably mounted in the base and extending from the base into the chamber and at least one mixing element mounted to the shaft, the shaft configured to be driven by a motor magnetically coupled to the shaft and external to the lower portion of the chamber. The support structure is connected to the mixing system such that the shaft is moveable therein and configured to cooperate with an external structure to provide support for the shaft.

Abstract: A filter holding device (1; 41; 75; 81) arranged for providing at least one filter (7; 43a, 43b; 85) inside a flexible bag (3; 71), said filter holding device comprising attaching means (19a-d, 21a-d; 79; 83) for attaching the filter holding device to an inner surface of the flexible bag such that the at least one filter held by the filter holding device is provided at a distance from the inner surface of the flexible bag.

Abstract: A connection clamping device (21; 61; 81; 131; 151) arranged for connecting a flexible tube (1) to a barbed end (5b) of a connection means (5), whereby the barbed end (5b) comprises a barb (9) and the connection means during connection is arranged to protrude into an end of the flexible tube (1), characterized in that said connection clamping device comprises two halves (23a, 23b) which, during connection when the connection means protrudes into an end of the flexible tube, are arranged to be connected and locked to each other by a locking mechanism (33a,33b,34a,34b; 63a, 63b, 64a, 64b) around the connection means (5) and the flexible tube (1), such that the flexible tube (1) is compressed against the connection means (5) by a rib (41a, 41b) provided on the inner circumference of the connection clamping device.

Abstract: The present invention provides a system for the insertion of a pre-sterilized sensor probe into a sterile vessel. The system of the invention provides a reliable and straightforward way to insert one or more sterile probes into a sterile vessel. The present invention also provides a sterile vessel that includes one or more of the systems of the invention. The sterile vessel can be a flexible or semi-rigid bag or tubing of the type typically used for carrying out biochemical and/or biological processes and/or manipulating liquids and other products of such processes. Furthermore, the present invention provides a method for aseptically inserting a probe into a sterile vessel where the method makes use of the system of the invention.

Abstract: The invention discloses a plastic bag comprising: at least one longitudinal end weld (112), wherein the end weld comprises an elongate transversely-oriented weld (114) bounded by a first pair (116) and a second pair (118) of coterminous oblique welds (116a, 116b, 118a, 118b) extending along an oblique angle from the first (114a) and second (114b) ends of transversely-oriented weld (114), respectively, such that each pair of oblique welds (116a, 116b, 118a, 118b) forms a first (120a) and second (120b) apex, respectively, adjacent its respective end (114a, 114b) of the transversely-oriented weld (114), a first (122) and second (124) planar gusset attached to the bag (110) along or within the first (116a, 116b) and second (118a, 118b) pair of oblique welds so as to overlie the first (120a) and second (120b) apex, respectively.

Abstract: A bioreactor system and packaging is provided. The bioreactor system includes a vessel for housing biomaterials for processing and a support structure. The vessel includes a flexible material defining a chamber and a mixing system positioned within the chamber. The mixing system includes an agitator for imparting motion and mixing to the contents of the vessel and includes a base affixed to the flexible material at a base section of the chamber, a shaft moveably mounted in the base and extending from the base into the chamber and at least one mixing element mounted to the shaft, the shaft configured to be driven by a motor magnetically coupled to the shaft and external to the lower portion of the chamber. The support structure is connected to the mixing system such that the shaft is moveable therein and configured to cooperate with an external structure to provide support for the shaft.

Abstract: The invention discloses a bioreactor assembly comprising: a) a plurality of cultivation bags; b) at least one flexible temperature control bag comprising a thermostating fluid, and; c) a tray adapted to rock back and forth around at least one axis, wherein the flexible temperature control bag is mounted on the tray and the cultivation bags are located on the flexible temperature control bag.