Abstract: A method for sequencing a nucleic acid molecule includes providing a sequencing cell (200) having a nanopore (216) in a membrane (214) that resides over a well (205), a first electrode (202) at a bottom of the well, a second electrode (210) in a chamber (215) above the membrane, and an electrolyte (206, 208) in the well (205) and the chamber (215). The first electrode (202) is configured to facilitate non-Faradaic conduction of ionic current and forms a capacitance with ions in the electrolyte. A first voltage signal (1310) is applied across the first and second electrodes (202, 210), thereby creating a force that moves a nucleic acid molecule in the sequence cell through the nanopore (216). The first voltage signal (1310) increases to compensate for changes in the capacitance at the first electrode (202) during application of the first voltage signal.

Abstract: Provided herein are methods and compositions for detecting and/or quantitating target analytes, including nucleic acids and polypeptides, using nanopore detectable barcodes.

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and insert-ing the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract: There is provided a system and method for user generated gameplay in chat. The system including a memory storing a text and multimedia messaging software application, and a processor configured to execute the text and multimedia messaging software application to initiate a message exchange session with a remote mobile device, in response to a request received from a user of the mobile device, the message exchange session displaying a user interface for exchanging text and multimedia messages, provide a plurality of games for selection by the user to be played during the message exchange session and within the user interface for exchanging text and multimedia messages, receive a selection of one of the plurality of games by the user after initiating the message exchange session, and initiate the one of the plurality of games for playing within the user interface for exchanging text and multimedia messages.

Abstract: Methods for recovering solvent from biomass are disclosed herein. In one example, the method can include exerting pressure on wetted plant material to force liquid solvent from the plant matter so the solvent can be collected for reuse. The method can also include flowing pressurized gas through the wetted plant matter, thereby forcing liquid solvent from the plant material so the solvent can be collected for reuse.

Abstract: Apparatuses for recovering solvent from biomass are disclosed herein. In one example, the apparatus can include a mechanical press configured to exert pressure on wetted plant material to force liquid solvent from the plant matter so the solvent can be collected for reuse. The apparatus can also be configured to flow pressurized gas through the wetted plant matter, thereby forcing liquid solvent from the plant material so the solvent can be collected for reuse.

Abstract: Provided is a method for detecting a target molecule. The method includes providing a chip, the chip including a nanopore in a membrane that is disposed adjacent to or in proximity to a sensing electrode. A nucleic acid molecule is then directed through the nanopore, the nucleic acid molecule being associated with a reporter molecule. The nucleic acid molecule also includes an address region and a probe region, the reporter molecule being associated with the nucleic acid molecule at the probe region. The reporter molecule is also coupled to a target molecule. While the nucleic acid molecule is directed through the nanopore, the address region can be sequenced to determine a nucleic acid sequence of the address region. The target molecule can then be identified, with the aid of a computer processor, based upon the nucleic acid sequence of the address region.

Abstract: Provided are methods of nucleic acid sample preparation for analysis, including analysis using nanopore sequencing applications. In particular provided are methods that allow for the creation of circular and linear DNA samples that contain asymmetric ends to ligate different and desired adaptors to the end of the sample, methods to create single nucleic acid molecules that automatically form concatemers for higher sequencing throughput; methods to create multiple copies (temperature dependent, multi-primed, open-fold replication) of one original nucleic acid sample, or to create single nucleic acid molecules containing multiple copies of more than one original nucleic acid sample (concatemerized samples) for improved accuracy and throughput are described; and methods for improved modified base calling.

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

Abstract: This disclosure provides systems and methods for attaching nanopore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides.

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract: Systems and methods are disclosed for detecting tremors in a subject. One method comprises receiving data from a digital device, the data comprising a plurality of digital device positions and a plurality of timestamps, each timestamp in the plurality of timestamps being associated with a digital device position in the plurality of digital device positions. The method further comprises determining a plurality of frequencies of hand movements of the subject based on the plurality of digital device positions and plurality of timestamps. The method further comprises determining a subportion of the data corresponding to frequencies of hand movements above a low tremor threshold, and determining a magnitude of tremors of the subject's hand based, at least in part, on the subportion of the data.

Abstract: A method includes receiving data representing graphomotor motion during a succession of executions of graphomotor diagnostic tasks performed in a medical context by a subject, processing the received data using a computer, including determining a first set of quantitative features from a first execution of a task by the subject, and determining a second set of quantitative features from a second execution of a task by the subject, determining one or more metrics based on a comparison to the successive executions, including using at least the first set of quantitative features and the second set of quantitative features to determine said metrics, and providing a diagnostic report associated with neurocognitive mechanisms underlying the subject's execution of the tasks based on the determined metrics.

Abstract: This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to compositions, methods, and systems for sequencing nucleic acids using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

Abstract: A method includes receiving data representing graphomotor motion during a succession of executions of graphomotor diagnostic tasks performed in a medical context by a subject, processing the received data using a computer, including determining a first set of quantitative features from a first execution of a task by the subject, and determining a second set of quantitative features from a second execution of a task by the subject, determining one or more metrics based on a comparison to the successive executions, including using at least the first set of quantitative features and the second set of quantitative features to determine said metrics, and providing a diagnostic report associated with neurocognitive mechanisms underlying the subject's execution of the tasks based on the determined metrics.

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract: Systems and methods are disclosed for ranking electronic content using a trained topic model to correlate a collection of source content to externally specified target content. Unstructured content is converted to elemental sub-content or interrelated sub-content. A probability vector for the converted externally specified content is generated by use of trained topic model. The externally specified topic probability vector is correlated against a collection of source content, previously converted to vectors that were generated using the same topic model, and a plurality of correlation methods. Rank ordered correlation results are merged to provide the user with a ranked set of source content. Source content from the ranked results can be fed back into the system to adjust the target vector.

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.