Abstract: A device and methods for detecting the effect of compounds on an organism are provided. Furthermore, the device and methods disclosed herein allow for the fractionation of complex samples and the isolation of one or more organisms for the samples. The device and methods also allow for the study of development of the organism.

Abstract: The present invention relates to methods, compositions and kits related to a novel in vitro assay for a high-capacity and high-throughput method for measuring the ability of cancer cells to migrate in a three-dimensional cellular assay. The three-dimensional cellular invasion assay provides a method for determining and quantitating the metastatic potential and invasive capacity of a cancer cell. Other aspects of the invention further relate to the use of the in vitro assay to screen for agents and compounds capable of inhibiting intravasation, and thereby modulating the metastatic potential of cancer cells. The methods, compositions and three-dimensional assay provide a highly sensitive assay system capable of mimicking the in vivo cellular and molecular interactions required for successful completion of intravasation.

Abstract: A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.

Abstract: The present invention generally relates to amplification of biological entities, for example, for phage display. In one aspect, members of a library of biological entities are encapsulated in separate compartments (e.g., in separate microfluidic droplets) and amplified. As a specific example, by putting members of a phage display library into microfluidic droplets such that no droplet contains more than one member of the library, the library can be amplified without any substantial changes in growth rates or population distributions, or other artifacts created due to differences in growth rates or amplification between different members of the library. In some cases, the volume of the compartments can be used to control the copy number of a biological entity during amplification. In certain cases, biological entities with different amplification rates can be amplified independently of each other.

Abstract: A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.

Abstract: A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.

Abstract: Three-dimensional cellular arrays, methods of making three-dimensional cellular arrays, and methods of identifying agents using the arrays are disclosed.

Abstract: A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.