Patents by Inventor Reginald Donovan Smith
Reginald Donovan Smith has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9469839Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.Type: GrantFiled: June 29, 2009Date of Patent: October 18, 2016Assignee: General Electric CompanyInventors: Slawomir Rubinsztajn, Prameela Susarla, Anton Beletskii, Reginald Donovan Smith, Brian Polizzotti
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Patent number: 9453196Abstract: A method of culturing adherent cells is provided, wherein the method comprises providing a carrier for growing the cells, comprising one or more surfaces; and one or more relief features/indentations are present on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features/indentations has a height above the surfaces in a range from about 2 to 200 ?m, and width in a range from about 20 to 200 ?m; seeding the cells on the carriers; and growing the cells on the carrier.Type: GrantFiled: November 2, 2011Date of Patent: September 27, 2016Assignee: GENERAL ELECTRIC COMPANYInventors: Reginald Donovan Smith, Scott Michael Miller, Yosang Yoon, Slawomir Rubinsztajn, Prameela Susarla
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Publication number: 20160252434Abstract: Provided herein are kit for providing a gamma sterilized aqueous dextran solution that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation while maintaining sufficient molecular weight to act as a red blood cell aggregate. Also provided are methods of use.Type: ApplicationFiled: May 9, 2016Publication date: September 1, 2016Inventors: Reginald Donovan Smith, Patrick Joseph McCloskey
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Publication number: 20160123848Abstract: A semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Further, the recovery conduit includes a first port and a second port, where the first port of the recovery conduit is configured to be operatively coupled to the sample source. Also, the second port of the recovery conduit is operatively coupled to at least a portion of the sampling conduit. Moreover, the one or more sampling kits are operatively coupled to the sampling conduit, and the pumping device is operatively coupled to the sampling conduit.Type: ApplicationFiled: October 31, 2014Publication date: May 5, 2016Inventors: Weston Blaine Griffin, Chengkun Zhang, Kenneth Roger Conway, Reginald Donovan Smith
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Publication number: 20160122798Abstract: A sampling assembly configured to be coupled to a sample source and facilitate aseptic sampling at one or more instances in time is provided. Further, the sampling assembly includes a first conduit having first and second ports, where the first port is configured to be coupled to the sample source. The sampling assembly also includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connector junctions. Also, each of the sub-ports is in fluidic communication with the first conduit. The sampling assembly also includes a plurality of sampling kits and one or more pumping devices. Further, each sampling kit is operatively coupled to a respective sub-port of a corresponding sub-conduit. Moreover, the one or more pumping devices are operatively and aseptically coupled to the second port of the first conduit.Type: ApplicationFiled: October 31, 2014Publication date: May 5, 2016Inventors: Chengkun Zhang, Kenneth Roger Conway, Donald Joseph Buckley, Eugene Pauling Boden, Weston Blaine Griffin, Anshika Bajaj, Reginald Donovan Smith
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Publication number: 20160053296Abstract: A sampling assembly configured to be coupled to a sample source is provided. The sampling assembly is configured to facilitate aseptic sampling at one or more instances in time. The sampling assembly includes a first conduit having a first port and a second port, where the first port is configured to be coupled to the sample source, and where the second port is configured to be hermetically sealed. The sampling assembly further includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connection points, and where each of the sub-ports is in fluidic communication with the first conduit. Moreover, the sampling assembly includes a plurality of sampling kits, where each sampling kit of the plurality of sampling kits is operatively connected to a respective sub-port of a corresponding sub-conduit.Type: ApplicationFiled: August 19, 2014Publication date: February 25, 2016Inventors: Chengkun Zhang, Kenneth Roger Conway, Donald Joseph Buckley, Eugene Pauling Boden, Weston Blaine Griffin, Anshika Bajaj, Reginald Donovan Smith, Zhipeng Zhang
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Publication number: 20160040114Abstract: A coupling device configured to form a sample access assembly is provided. The sample access assembly is configured to house a sample. The coupling device includes a heating component and a separating component. Further, the separating component is configured to separate portions of first and second containers that form first and second compartments of the sample access assembly. Moreover, the heating component is configured to heat at least a portion of the sample.Type: ApplicationFiled: August 7, 2014Publication date: February 11, 2016Inventors: Ying Mao, Kenneth Roger Conway, Weston Blaine Griffin, Reginald Donovan Smith, Evelina Roxana Loghin, Vandana Keskar, Chengkun Zhang, Zhipeng Zhang
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Publication number: 20150299657Abstract: Provided herein are gamma stable dextran solutions and kits that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation and maintain sufficient molecular weight to act as a red blood cell aggregant. Further provided are methods of use.Type: ApplicationFiled: April 16, 2014Publication date: October 22, 2015Applicant: GENERAL ELECTRIC COMPANYInventors: Patrick Joseph McCloskey, James Edward Pickett, Gregory Daryll Goddard, Reginald Donovan Smith, Anshika Bajaj
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Publication number: 20150093739Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.Type: ApplicationFiled: December 9, 2014Publication date: April 2, 2015Inventors: Nichole Lea Wood, Reginald Donovan Smith, Hongyi Zhou, Liming Yu, Brian David Polizzotti, Peter D. Miller
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Patent number: 8993322Abstract: Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.Type: GrantFiled: February 25, 2013Date of Patent: March 31, 2015Assignee: General Electric CompanyInventors: Slawomir Rubinsztajn, Prameela Susarla, Reginald Donovan Smith, Evelina Roxana Loghin
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Patent number: 8961787Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.Type: GrantFiled: December 1, 2008Date of Patent: February 24, 2015Assignee: General Electric CompanyInventors: Nichole Lea Wood, Reginald Donovan Smith, Hongyi Zhou, Liming Yu, Brian Polizzotti, Peter Miller
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Patent number: 8399252Abstract: Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.Type: GrantFiled: September 30, 2009Date of Patent: March 19, 2013Assignee: General Electric CompanyInventors: Slawomir Rubinsztain, Prameela Susarla, Reginald Donovan Smith, Evelina Roxana Loghin
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Publication number: 20120156773Abstract: A method of culturing adherent cells is provided, wherein the method comprises providing a carrier for growing the cells, comprising one or more surfaces; and one or more relief features/indentations are present on one or more of the surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.012 mm to 0.5 mm; and wherein each of the relief features/indentations has a height above the surfaces in a range from about 2 to 200 ?m, and width in a range from about 20 to 200 ?m; seeding the cells on the carriers; and growing the cells on the carrier.Type: ApplicationFiled: November 2, 2011Publication date: June 21, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: Reginald Donovan Smith, Scott Michael Miller, Yosang Yoon, Slawomir Rubinsztajn, Prameela Susarla
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Publication number: 20120156777Abstract: A carrier for growing adherent cells is provided, wherein the carrier comprises one or more outer surfaces; and one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. The carrier may comprise a single indentation or ‘cup’ like structure, or may comprise a plurality of indentations. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.Type: ApplicationFiled: December 16, 2010Publication date: June 21, 2012Applicant: GENERAL ELECTRIC COMPANYInventors: Arvind Rangarajan, Prameela Susarla, Scott Michael Miller, Reginald Donovan Smith, Slawomir Rubinsztajn, Anton Beletskii
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Publication number: 20110070596Abstract: Agents and methods for qualitative and quantitative analysis a protein complex or protein complexes using isotope-labeled symmetrical bifunctional crosslinkers and mass spectrometry are provided. Targeting moieties, cell permeability moieties, or affinity moieties, may be appended to the bifunctional crosslinkers. The isotope-labeled symmetrical bifunctional crosslinkers may be used in a kit or as a library.Type: ApplicationFiled: February 15, 2010Publication date: March 24, 2011Applicant: GENERAL ELECTRIC COMPANYInventors: Nichole Lea Wood, Mohan Mark Amaratunga, Hans Grade, Faisal Ahmed Syud, Anup Sood, Reginald Donovan Smith, Ayse Betul Dinc, Amy Casey Williams, Anthony John Murray, Gregory Daryll Goddard
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Publication number: 20100330674Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.Type: ApplicationFiled: June 29, 2009Publication date: December 30, 2010Applicant: GENERAL ELECTRIC COMPANYInventors: Slawomir Rubinsztajn, Prameela Susarla, Anton Beletskii, Reginald Donovan Smith, Brian Polizzotti
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Publication number: 20100151438Abstract: The methods and kits provide sedimentation-enhancing agents that significantly increase the efficiency of cell-sedimentation. They also increase the efficiency of blood cell separation methods and thereby increase the recovery of total nucleated cells.Type: ApplicationFiled: December 12, 2008Publication date: June 17, 2010Applicant: GENERAL ELECTRIC COMPANYInventors: Liming Yu, Hongyi Zhou, Nichole Lee Wood, Anup Sood, Reginald Donovan Smith, Brian David Polizzotti
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Publication number: 20100133181Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.Type: ApplicationFiled: December 1, 2008Publication date: June 3, 2010Applicant: General Electric CompanyInventors: Nichole Lea Wood, Reginald Donovan Smith, Hongyi Zhou, Liming Yu, Brian Polizzotti, Peter Miller
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Publication number: 20080308422Abstract: In one aspect, the invention provides methods for protein sample preparation for electrophoretic separation. The method comprises a step of providing a protein sample in solution, adding a chaotrope to the protein sample, adding a surfactant to the protein sample, wherein the final concentration of the surfactant in the solution is less than critical micelle concentration of the surfactant. In another aspect, the invention also provides methods of electrophoretic separation of protein samples that includes the protein sample preparation method as described herein. In yet another aspect, the invention provides a loading buffer solution composition for protein sample preparation that includes a surfactant in solution at a final concentration that is less than critical micelle concentration of the surfactant, and; a chaotrope.Type: ApplicationFiled: June 13, 2007Publication date: December 18, 2008Applicant: GENERAL ELECTRIC COMPANYInventors: Reginald Donovan Smith, Erin Jean Finehout