Patents by Inventor Richard C. Terry

Richard C. Terry has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of selectively sequencing amplicons in a biological sample
    Patent number: 12264358
    Abstract: Methods of selectively sequencing amplicons in a biological sample are provided.
    Type: Grant
    Filed: May 2, 2024
    Date of Patent: April 1, 2025
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Jehyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Flourescent in Situ Sequ
    Publication number: 20240327901
    Abstract: The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.
    Type: Application
    Filed: May 2, 2024
    Publication date: October 3, 2024
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry, Benjamin W. Pruitt, Brian M. Turczyk
  • Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix
    Publication number: 20240301468
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Application
    Filed: May 2, 2024
    Publication date: September 12, 2024
    Inventors: George M. Church, Je-hyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix
    Publication number: 20240287582
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Application
    Filed: May 2, 2024
    Publication date: August 29, 2024
    Inventors: George M. Church, Je-hyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Method and Apparatus for Volumetric Imaging
    Publication number: 20230279484
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Application
    Filed: September 30, 2022
    Publication date: September 7, 2023
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-Hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Methods and Compositions for the Single Tube Preparation of Sequencing Libraries Using Cas9
    Publication number: 20230272373
    Abstract: Methods and compositions of single tube preparation of sequencing libraries from a target DNA are provided. The methods include contacting the DNA with a composition comprising Cas9 endonuclease, a first and a second guide RNAs, a ligase, and sequencing adapters, subjecting the composition to thermal cycling to cleave the DNA at the sites flanking the regions of interest by the RNA guided endonuclease, and subjecting the composition to a temperature to allow ligation of the cleaved DNA fragments including the regions of interest with the sequencing adapters to generate the sequencing libraries.
    Type: Application
    Filed: July 25, 2022
    Publication date: August 31, 2023
    Inventors: George M. Church, Benjamin W. Pruitt, Richard C. Terry
  • Methods of Combining the Detection of Biomolecules Into a Single Assay Using Fluorescent In Situ Sequencing
    Publication number: 20230146821
    Abstract: The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.
    Type: Application
    Filed: August 10, 2022
    Publication date: May 11, 2023
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry
  • Method and apparatus for volumetric imaging
    Patent number: 11542554
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Grant
    Filed: August 6, 2021
    Date of Patent: January 3, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-Hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Spatial sequencing of nucleic acids using DNA origami probes
    Patent number: 11473139
    Abstract: A method of sequencing nucleic acids is provided using DNA origami as a barcode for a nucleic acid probe.
    Type: Grant
    Filed: March 26, 2020
    Date of Patent: October 18, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault
  • Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
    Patent number: 11447807
    Abstract: The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: September 20, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry
  • Sequencing by Structure Assembly
    Publication number: 20220251642
    Abstract: A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.
    Type: Application
    Filed: April 8, 2022
    Publication date: August 11, 2022
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault, Francois Vigneault
  • Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix
    Publication number: 20220228196
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Application
    Filed: March 30, 2022
    Publication date: July 21, 2022
    Inventors: George M. Church, Je-Hyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Sequencing by structure assembly
    Patent number: 11312992
    Abstract: A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.
    Type: Grant
    Filed: November 25, 2019
    Date of Patent: April 26, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault, Francois Vigneault
  • Method for generating a three-dimensional nucleic acid containing matrix
    Patent number: 11299767
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Grant
    Filed: June 30, 2021
    Date of Patent: April 12, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Je-Hyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Methods and Compositions for the Single Tube Preparation of Sequencing Libraries Using Cas9
    Publication number: 20220106591
    Abstract: Methods and compositions of single tube preparation of sequencing libraries from a target DNA are provided. The methods include contacting the DNA with a composition comprising Cas9 endonuclease, a first and a second guide RNAs, a ligase, and sequencing adapters, subjecting the composition to thermal cycling to cleave the DNA at the sites flanking the regions of interest by the RNA guided endonuclease, and subjecting the composition to a temperature to allow ligation of the cleaved DNA fragments including the regions of interest with the sequencing adapters to generate the sequencing libraries.
    Type: Application
    Filed: December 16, 2021
    Publication date: April 7, 2022
    Inventors: George M. Church, Benjamin W. Pruitt, Richard C. Terry
  • Method and Apparatus for Volumetric Imaging
    Publication number: 20210363579
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Application
    Filed: August 6, 2021
    Publication date: November 25, 2021
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-Hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Method for Generating A Three-Dimensional Nucleic Acid Containing Matrix
    Publication number: 20210324450
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Application
    Filed: June 30, 2021
    Publication date: October 21, 2021
    Inventors: George M. Church, Je-Hyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
    Patent number: 11118220
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Grant
    Filed: April 17, 2019
    Date of Patent: September 14, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Methods and Compositions for the Single Tube Preparation of Sequencing Libraries Using Cas9
    Publication number: 20210277389
    Abstract: Methods and compositions of single tube preparation of sequencing libraries from a target DNA are provided. The methods include contacting the DNA with a composition comprising Cas9 endonuclease, a first and a second guide RNAs, a ligase, and sequencing adapters, subjecting the composition to thermal cycling to cleave the DNA at the sites flanking the regions of interest by the RNA guided endonuclease, and subjecting the composition to a temperature to allow ligation of the cleaved DNA fragments including the regions of interest with the sequencing adapters to generate the sequencing libraries.
    Type: Application
    Filed: May 3, 2021
    Publication date: September 9, 2021
    Inventors: George M. Church, Benjamin W. Pruitt, Richard C. Terry
  • Compositions and methods for analyte detection
    Patent number: 11111521
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Grant
    Filed: December 15, 2020
    Date of Patent: September 7, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Je-hyuk Lee, Richard C. Terry, Evan R. Daugharthy