Abstract: A method for facilitating a delivery of a molecule into an interior space of a cell includes the steps of introducing a molecule into a target tissue comprising a cell and applying a substantially continuous low-level electric field to the target tissue. The field is applied for a duration sufficient to effect a change in porosity the cell of the target tissue sufficient to facilitate an entry of a desired molecule into an interior of the cell.

Abstract: Devices, systems and methods for regulating the distribution of electricity at events such as trade shows to prevent exhibitors from utilizing electricity not purchased from the convention center or other entity controlling electricity distribution, including lockable electric outlet boxes having a lockable fuse holder containing a fuse that restricts or limits a specific electrical outlet box or group of outlet boxes to a maximum electrical current or amperage.

Abstract: A device for manipulating a molecule in vivo relative to a target tissue includes at least one elongated member having at least two discrete and separately activatable electrodes separated by an insulating material interposed therebetween. The electrodes are configures to establish at least one of a first electromagnetic field between selected electrodes sufficient to manipulate a molecule relative to a target tissue and a second, typically higher-level, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. A third electromagnetic field may also be applied to cause further translation of the molecule into an electropermeabilized cell and/or manipulated with respect to the tissue. Thus three-dimensional manipulation of the molecule relative to the target tissue may be effected to optimize a desired positioning thereof, such as entry into a cell.

Abstract: The present invention is a device for electromanipulation of chemical species in vivo relative to a target tissue including a nonconductive, conformable array base adapted to be placed coincident to the target tissue, a plurality of electrode elements projecting from the array base towards the target tissue, the electrode elements addressable individually, an electrical source coupled to the plurality of electrodes, a control means interposed between the electrical source and the plurality of electrode elements and in circuit communication therein, the control means adapted to establish an electrical potential between at least two electrodes, and a delivery means adapted to introduce chemical species to the target tissue.

Abstract: A method for facilitating a delivery of a molecule into an interior space of a cell includes the steps of introducing a molecule into a target tissue comprising a cell and applying a substantially continuous low-level electric field to the target tissue. The field is applied for a duration sufficient to effect a change in porosity the cell of the target tissue sufficient to facilitate an entry of a desired molecule into an interior of the cell.

Abstract: By maintaining a substantially constant total die power during the entire lifetime of sophisticated integrated circuits, the performance degradation may be reduced. Consequently, greatly reduced guard bands for parts classification may be used compared to conventional strategies in which significant performance degradation may occur when the integrated circuits are operated on the basis of a constant supply voltage.

Abstract: The electroporation system and method combine pulses having different characteristics for delivering molecules to cells in vivo. The pulses include a high-intensity pulse for inducing electroporation and a low-intensity pulse to induce electrophoretic molecule movement within an interstitial space, molecule adherence to a cell membrane, and electrophoretic movement of the molecule through the permeabilized membrane. The use of a high-intensity and a low-intensity pulse achieves improved delivery; reduction of intensity and/or duration of pulses for inducing electroporation; and decreased muscle stimulation, tissue damage, and patient discomfort.

Abstract: A flow sensor for determining the flow rate of a fluid therethrough. The flow sensor may include a chamber for the fluid to flow therethrough, a moveable magnet positioned within the chamber, a stationary magnet positioned about the chamber, and one or more sensors positioned about the chamber to determine the position of the moveable magnet therein.

Abstract: The present invention provides a system and method of directing, focusing, or concentrating electrical charges within a defined electric field so that these charges can be used to exert forces on cells and tissues in vivo and/or cell cultures in vitro. The present invention reduces and/or eliminates the damage at a target site that would normally be caused by an electrode that acts as a current source or sink to accomplish the same task.

Abstract: A flow sensor for determining the flow rate of a fluid therethrough. The flow sensor may include a chamber for the fluid to flow therethrough, a moveable magnet positioned within the chamber, a stationary magnet positioned about the chamber, and one or more sensors positioned about the chamber to determine the position of the moveable magnet therein.

Abstract: The electroporation system and method combine pulses having different characteristics for delivering molecules to cells in vivo. The pulses include a high-intensity pulse for inducing electroporation and a low-intensity pulse to induce electrophoretic molecule movement within an interstitial space, molecule adherence to a cell membrane, and electrophoretic movement of the molecule through the permeabilized membrane. The use of a high-intensity and a low-intensity pulse achieves improved delivery; reduction of intensity and/or duration of pulses for inducing electroporation; and decreased muscle stimulation, tissue damage, and patient discomfort.

Abstract: In accordance with the present invention is provided a novel tri-pronged electrode that produces minimal damage to deep brain or spinal cord regions. The apparatus and associated method of the present invention provides for a single insertion of a novel probe effective for both delivery of DNA and delivery of therapeutic electroporating current, thereby minimizing the potential damage to the nervous system.

Abstract: The present invention provides for a system and method whereby aspirin and acetic acid help to permeabilize cell membranes to allow exogenous molecules to gain access to the cell interior. As such, the present invention provides a low cost drug and gene delivery tool that can be applied in combination with other molecular delivery methods.

Abstract: Signal Transducer and Activator of Transcription (STAT) proteins have a fundamental role cell signaling, and are activated by a large number of cytokines and growth factors. One member of the STAT family, STAT3, has a critical role in oncogenesis. The present invention relates generally to disruption of the pathway of STAT3 signaling in the treatment of human cancer. STAT3 activation is shown to be present in diverse tumor cell lines and tumors, to promote oncogenesis, to inhibit apoptosis, and to reduce sensitivity to chemotherapeutic agents. Inhibition of STAT3 signaling induces apoptosis specifically in tumor cell lines, and increases sensitivity to chemotherapeutic agents. The invention relates more particularly to methods, compositions, means of administering such compositions, and means for identifying such compositions for the inhibition of STAT3 intracellular signaling in the treatment of human cancers.

Abstract: The present invention is a method of eliciting an antitumor effect in vivo comprising the steps of identifying a species representative of a treatment subject, identifying at least one non-coding nucleic acid sequence, introducing the at least one nucleic acid to at least one tumor in the treatment subject and applying an energy source to the at least one tumor. The energy source may comprise, but is not limited to, electrical, sonic, photonic, and microwave output.

Abstract: The present invention provides an apparatus and method that will accomplish molecular delivery and subsequent electroporation with a single apparatus without the need for separate molecule injection and placement of the electroporation applicators.

Abstract: Each LDIF entry of a directory tree is read, split to a domain of LDIF fragments (corresponding to backend servers) and written to each LDIF fragment. The split may be accomplished through a hash function, establishing, for that iteration of LDIF entry, a write file. The LDIF entry is appended to the write file. A subsequent LDIF entry is read. A corresponding LDIF fragment is determined, which need not be different from the LDIF fragment to which the first LDIF entry was written. The current LDIF entry is written to the currently selected write file. The process continues until all LDIF entries are exhausted from the directory tree. LDIF fragments are each copied to distinct backend servers, where, each LDIF fragment may be loaded into a distributed directory data structure.

Abstract: A system is presented for performing a directory operation within a distributed directory environment that includes distributed directory servers and a proxy server that acts as an intermediate agent between a client and the distributed directory environment. The proxy server sends requests to directory servers to collect information about group memberships for a user with respect to group entries within each portion of a distributed directory that is supported by each directory server. The proxy server sends the compiled information of group memberships for the user along with any directory operation that the proxy server requests on behalf of the user. A directory server receives the compiled information of group memberships along with a requested directory operation and then performs the requested directory operation with respect to its locally stored portion of the distributed directory information tree and with respect to the received information of group memberships for the user.

Abstract: In accordance with the present invention is provided a device and method for delivering DNA for the purpose of gene therapy to specific regions within and around the eye.

Abstract: A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.