Patents by Inventor Richard Heller
Richard Heller has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6937890Abstract: The manipulator includes a support and at least one member affixed to and extending away from the support. The member has at least two differentially activatable areas of conductivity. The members are configured to establish a first electromagnetic field in vivo between selected areas of conductivity sufficient to manipulate a molecule relative to a target tissue and to establish a second, typically higher, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. Restraining means are also described for restricting movement of the members with relation to each other. One method of using the device is for enhancing the delivery of a molecule into a tissue site; another is for poration of the tissue alone or in combination with the migration. The target tissue may include a tumor, organ, or wound site.Type: GrantFiled: January 30, 2001Date of Patent: August 30, 2005Assignee: University of South FloridaInventors: Mark J. Jaroszeski, Richard Gilbert, Richard Heller
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Patent number: 6929949Abstract: The present invention provides a method and apparatus adapted to facilitate the entry of a preselected molecule into the intracellular space of a cellular sample through the use of ions generated by a corona charge source. With the present method and apparatus, molecules are manipulated within cells and in the extracellular space surrounding the cells. Manipulation enhances the permeability of cell barriers to allow the subsequent introduction of molecules of interest into the interior of a cell.Type: GrantFiled: June 13, 2003Date of Patent: August 16, 2005Assignee: University of South FloridaInventors: Drew Hoff, Mark J. Jaroszeski, Richard Gilbert, Richard Heller
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Publication number: 20050076335Abstract: A method is presented for processing data in a multithreaded application to alleviate impaired or substandard performance conditions. Work items that are pending processing by the multithreaded application are placed into a data structure. The work items are processed by a plurality of threads within the multithreaded application in accordance with a first algorithm, e.g., first-in first-out (FIFO). A thread within the multithreaded application is configured apart from the plurality of threads such that it processes work items in accordance with a second algorithm that differs from the first algorithm, thereby avoiding the impairing condition. For example, the thread may process a pending work item only if it has a particular characteristic. The thread restricts its own processing of work items by intermittently evaluating workflow conditions for the plurality of threads; if the workflow conditions improve or are unimpaired, then the thread does not process any work items.Type: ApplicationFiled: October 2, 2003Publication date: April 7, 2005Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Mark Cavage, Kristin Hazlewood, Richard Heller, Gary Williams
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Publication number: 20050054969Abstract: The present invention is a device for electromanipulation of chemical species in vivo relative to a target tissue including a nonconductive, conformable array base adapted to be placed coincident to the target tissue, a plurality of electrode elements projecting from the array base towards the target tissue, the electrode elements addressable individually, an electrical source coupled to the plurality of electrodes, a control means interposed between the electrical source and the plurality of electrode elements and in circuit communication therein, the control means adapted to establish an electrical potential between at least two electrodes, and a delivery means adapted to introduce chemical species to the target tissue.Type: ApplicationFiled: September 10, 2003Publication date: March 10, 2005Applicant: UNIVERSITY OF SOUTH FLORIDAInventors: Andrew Hoff, Richard Gilbert, Richard Heller, Mark Jaroszeski
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Patent number: 6778853Abstract: A device for manipulating a molecule in vivo relative to a target tissue includes at least one elongated member having at least two discrete and separately activatable electrodes separated by an insulating material interposed therebetween. The electrodes are configured to establish at least one of a first electromagnetic field between selected electrodes sufficient to manipulate a molecule relative to a target tissue and a second, typically higher-level, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. A third electromagnetic field may also be applied to cause further translation of the molecule into an electropermeabilized cell and/or manipulated with respect to the tissue. Thus three-dimensional manipulation of the molecule relative to the target tissue may be effected to optimize a desired positioning thereof, such as entry into a cell.Type: GrantFiled: October 24, 2000Date of Patent: August 17, 2004Assignee: University of South FloridaInventors: Richard Heller, Richard Gilbert, Mark J. Jaroszeski
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Patent number: 6714816Abstract: The electroporation system and method combine pulses having different characteristics for delivering molecules to cells in vivo. The pulses include a high-intensity pulse for inducing electroporation and a low-intensity pulse to induce electrophoretic molecule movement within an interstitial space, molecule adherence to a cell membrane, and electrophoretic movement of the molecule through the permeabilized membrane. The use of a high-intensity and a low-intensity pulse achieves improved delivery; reduction of intensity and/or duration of pulses for inducing electroporation; and decreased muscle stimulation, tissue damage, and patient discomfort.Type: GrantFiled: February 22, 2000Date of Patent: March 30, 2004Assignee: University of South FloridaInventors: Richard Heller, Richard Gilbert, Mark J. Jaroszeski, Loree C. Heller, Melinda L. Lucas
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Publication number: 20030216784Abstract: The present invention is a method of electromanipulation for effecting substantially simultaneous electroporation and electromigration of molecules into cells by applying to a cellular target a preselected electrical waveform. The preselected electrical waveform may be formed of at least one curved or linear component either increasing or decreasing in amplitude as a function of time. In a preferred embodiment of the invention the at least one component has a duration no greater than five minutes and a maximum amplitude no greater than 10,000 V/cm. Alternatively, the waveform may also include a substantially constant amplitude component interposed between the increasing and decreasing components. The substantially constant amplitude component may also be applied prior or subsequent to the at least one component.Type: ApplicationFiled: August 5, 2002Publication date: November 20, 2003Inventors: Richard Heller, Richard Gilbert, Mark J. Jaroszeski
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Publication number: 20030141294Abstract: An apparatus for producing cell electrofusion is provided. The apparatus for producing cell fusion, the apparatus including a chamber including an insert and a cuvette, the chamber also includes a substrate, a fluid medium for containing cells to be fused in said chamber, a device for inducing migration of cells in said chamber toward one side of said substrate, and a device for inducing fusion of at least a portion of the cells.Type: ApplicationFiled: January 28, 2002Publication date: July 31, 2003Inventors: Mark J. Jaroszeski, Richard A. Gilbert, Richard Heller
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Patent number: 6569149Abstract: A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.Type: GrantFiled: June 21, 2002Date of Patent: May 27, 2003Assignees: Genetronics, Inc., The University of South Florida Research Foundation, Inc.Inventors: S. B. Dev, Gunter A. Hofmann, Richard A. Gilbert, Richard Heller, Mark J. Jaroszeski
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Publication number: 20030044985Abstract: A method for facilitating a delivery of a molecule into an interior space of a cell includes the steps of introducing a molecule into a target tissue comprising a cell and applying a substantially continuous low-level electric field to the target tissue. The field is applied for a duration sufficient to effect a change in porosity the cell of the target tissue sufficient to facilitate an entry of a desired molecule into an interior of the cell.Type: ApplicationFiled: August 24, 2001Publication date: March 6, 2003Inventors: Mark J. Jaroszeski, Richard Gilbert, Richard Heller
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Publication number: 20020198485Abstract: A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.Type: ApplicationFiled: June 21, 2002Publication date: December 26, 2002Applicant: GENETRONICS, INC.Inventors: Sukhendu B. Dev, Gunter A. Hofmann, Richard A. Gilbert, Richard Heller, Mark J. Jaroszeski
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Patent number: 6451002Abstract: A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.Type: GrantFiled: October 25, 1999Date of Patent: September 17, 2002Assignee: Genetronics, Inc.Inventors: Sukhendo B. Dev, Gunter A. Hofmann, Richard A. Gilbert, Yasuhiko Hayakawa, Richard Heller, Mark J. Jaroszeski
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Patent number: 6418341Abstract: A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.Type: GrantFiled: April 18, 2000Date of Patent: July 9, 2002Assignee: Genetronics, Inc.Inventors: Gunter A. Hofmann, Richard A. Gilbert, Richard Heller, Mark J. Jaroszeski
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Publication number: 20020042588Abstract: The manipulator includes a support and at least one member affixed to and extending away from the support. The member has at least two differentially activatable areas of conductivity. The members are configured to establish a first electromagnetic field in vivo between selected areas of conductivity sufficient to manipulate a molecule relative to a target tissue and to establish a second, typically higher, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. Restraining means are also described for restricting movement of the members with relation to each other. One method of using the device is for enhancing the delivery of a molecule into a tissue site; another is for poration of the tissue alone or in combination with the migration. The target tissue may include a tumor, organ, or wound site.Type: ApplicationFiled: January 30, 2001Publication date: April 11, 2002Inventors: Mark J. Jaroszeski, Richard Gilbert, Richard Heller
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Patent number: 6355485Abstract: A new and useful apparatus for producing cell electrofusion is provided. The apparatus comprises: a. a chamber with a substrate disposed therein, b. means for directing the cells to be fused toward one side of the substrate; and c. a device for inducing fusion of the portion of the cells.Type: GrantFiled: July 14, 2000Date of Patent: March 12, 2002Assignee: University of South FloridaInventors: Mark J. Jaroszeski, Richard A. Gilbert, Richard Heller
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Patent number: 6314316Abstract: The manipulator includes a support and at least one member affixed to and extending away from the support. The member has at least two differentially activatable electrodes. The electrodes are configured to establish a first electromagnetic field in vivo between selected electrodes sufficient to manipulate a molecule relative to a target tissue and to establish a second, typically higher, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. One method of using the device is for enhancing the delivery of a molecule into a tissue site. In a related embodiment the device may be used to cause the electromigration of at least two components of a multicomponent reactive system into apposition to permit a reaction to occur at a desired target tissue site. The target tissue may comprise a tumor, organ, or wound site.Type: GrantFiled: December 17, 1998Date of Patent: November 6, 2001Assignee: University of South FloridaInventors: Richard Gilbert, Mark Jaroszeski, Richard Heller
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Patent number: 6221665Abstract: A new and useful apparatus for producing cell electrofusion is provided. The apparatus comprises: a. a chamber with a substrate disposed therein, b. means for directing the cells to be fused toward one side of the substrate in so that the cells in the fluid medium are retained against the one side of the substrate with a significant portion of the cells in cell-to-cell contact with each other along said one side of the substrate, and c. a device for inducing fusion of the portion of the cells in cell-to-cell contact with each other along the predetermined portion of the one side of the substrate.Type: GrantFiled: June 8, 1999Date of Patent: April 24, 2001Assignee: University of South FloridaInventors: Mark J. Jaroszeski, Richard A. Gilbert, Richard Heller
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Patent number: 6135990Abstract: A device for manipulating a molecule in vivo relative to a target tissue in three dimensions includes a support and at least one member affixed to and extending away from the support. The member has at least two discrete and separately activatable electrodes. The electrodes are configured to establish a first electromagnetic field between selected electrodes sufficient to manipulate a molecule relative to a target tissue. The electrodes are further configured to establish a second, typically higher-level, electromagnetic field sufficient to cause transient permeability of a cell membrane within the target tissue. A third electromagnetic field may also be applied to cause further translation of the molecule into an electropermeabilized cell and/or manipulated with respect to the tissue. Thus three-dimensional manipulation of the molecule relative to the target tissue is effected to optimize a desired positioning thereof, such as entry into a cell.Type: GrantFiled: December 17, 1998Date of Patent: October 24, 2000Assignee: University of South FloridaInventors: Richard Heller, Richard Gilbert, Mark Jaroszeski
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Patent number: 5830460Abstract: A method of producing a sustained localized brain immunosuppressive effect in localized tissues is achieved by transplanting Sertoli cells proximate to the brain tissue.Type: GrantFiled: March 13, 1995Date of Patent: November 3, 1998Assignee: University of South FloridaInventors: Paul R. Sanberg, Don F. Cameron, Cesario V. Borlongan, Richard Heller
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Patent number: 5827736Abstract: A purified and isolated Sertoli cell and secretory cell hybrid, or an aggregate of these two cells, wherein the secretory cells preferably are pancreatic islet cells and chromaffin cells characterized by beinga) capable of survival in situ after transplantation;b) able to provide immunoprotection for the hybrid cells when transplanted; andc) able to provide a mechanism for prolonged viability and cellular functionality of the transplanted hybrid cells wherein the hybrid maintains both the immunoprotection characteristics of the Sertoli cell and the secretory function of the secretory cell.Type: GrantFiled: January 9, 1997Date of Patent: October 27, 1998Assignee: University of South FloridaInventors: Richard Heller, Don F. Cameron, Paul R. Sanberg, Mark J. Jaroszeski