Patents by Inventor Richard Jude Samulski

Richard Jude Samulski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20100310516
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Application
    Filed: August 2, 2010
    Publication date: December 9, 2010
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Publication number: 20100303776
    Abstract: The present invention provides a cell comprising a first heterologous nucleic acid construct and a second heterologous nucleic acid construct, wherein each of said first and second heterologous nucleic acid constructs comprises: A. a first nucleotide sequence encoding a nucleotide sequence of interest (NOI); and B.
    Type: Application
    Filed: April 16, 2010
    Publication date: December 2, 2010
    Inventors: Richard Jude Samulski, Kyson Xiaohuai Chou
  • Patent number: 7790154
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Grant
    Filed: January 19, 2007
    Date of Patent: September 7, 2010
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Publication number: 20100137211
    Abstract: The present invention provides methods and compositions for treating blood clotting factor disorders and/or reducing bleeding-associated joint damage by treatments delivered to the joint in a subject.
    Type: Application
    Filed: April 11, 2008
    Publication date: June 3, 2010
    Inventors: Paul E. Monahan, Richard Jude Samulski, Darrel W. Stafford
  • Publication number: 20090215879
    Abstract: The invention provides modified AAV capsid proteins comprising substitutions in the HI loop. Suitable substitutions include affinity tags, sequences that facilitate detection and/or targeting peptides. The invention also provides virus capsids and virus vectors comprising the modified AAV capsid proteins and methods of using the same. Further provided are methods of purifying the modified AAV capsid subunits, virus capsids and virus vectors of the invention.
    Type: Application
    Filed: February 12, 2009
    Publication date: August 27, 2009
    Inventors: NINA DIPRIMIO, RICHARD JUDE SAMULSKI
  • Publication number: 20090104155
    Abstract: The present invention relates to methods of delivering nucleic acids to connective tissue cells and to methods of treating connective tissue disorders; in particular, the invention provides methods of delivering nucleic acids to connective tissue cells and methods of treating connective tissue disorders using parvovirus vectors.
    Type: Application
    Filed: April 20, 2007
    Publication date: April 23, 2009
    Inventors: Laurie R. Goodrich, Richard Jude Samulski, Vivian W. Choi
  • Publication number: 20090004743
    Abstract: A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
    Type: Application
    Filed: June 17, 2008
    Publication date: January 1, 2009
    Inventors: Forrest K. Ferrari, Xiao Xiao, Richard Jude Samulski
  • Patent number: 7465583
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Grant
    Filed: May 31, 2001
    Date of Patent: December 16, 2008
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Publication number: 20080269149
    Abstract: The present invention is based, in part, on the discovery that parvovirus (including AAV) capsids can be engineered to incorporate small, selective regions from other parvoviruses that confer desirable properties. The inventors have discovered that in some cases as little as a single amino acid insertion or substitution from a first parvovirus (e.g., an AAV) into the capsid structure of another parvovirus (e.g., an AAV) to create a chimeric parvovirus is sufficient to confer one or more of the desirable properties of the first parvovirus to the resulting chimeric parvovirus and/or to confer a property that is not exhibited by the first parvovirus or is present to a lesser extent.
    Type: Application
    Filed: December 15, 2005
    Publication date: October 30, 2008
    Inventors: Dawn E. Bowles, Chengwen Li, Joseph E. Rabinowitz, Josh Grieger, Mavis Agbandje-McKenna, Richard Jude Samulski
  • Patent number: 7439065
    Abstract: A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
    Type: Grant
    Filed: August 22, 2005
    Date of Patent: October 21, 2008
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Forrest K. Ferrari, Xiao Xiao, Richard Jude Samulski
  • Patent number: 7229823
    Abstract: The present invention relates to methods and compositions for increasing the production of high titre stocks of recombinant AAV (rAAV) through regulation of expression of the AAV REP and CAP proteins. The methods and compositions of the invention are based on the observation that the low level expression of the AAV REP protein increases the production of AAV viral capsid protein and efficiency of packaging resulting in production of higher titre recombinant viral stocks. The invention encompasses recombinant AAV vectors that direct the expression of AAV REP and CAP proteins and the use of such vectors for the production of novel stable cell lines capable of generating high titre rAAV vectors. The invention provides methods for regulating the expression of the AAV REP gene at the transcriptional and post-translational level.
    Type: Grant
    Filed: September 30, 2002
    Date of Patent: June 12, 2007
    Inventors: Richard Jude Samulski, Xiao Xiao, Richard Snyder
  • Patent number: 7201898
    Abstract: The invention uses recombinant parvoviruses, and particularly recombinant adeno-associated virus (rAAV) to deliver genes and DNA sequences for gene therapy following manipulation of the therapeutic virus for packaging and transport. The invention delivers therapeutic viral vectors via rAAV affixed to support matrixes (i.e., sutures, surgically implantable materials, grafts, and the like).
    Type: Grant
    Filed: June 1, 2001
    Date of Patent: April 10, 2007
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Paul E. Monahan, Richard Jude Samulski, Joseph E. Rabinowitz
  • Patent number: 7172893
    Abstract: The present invention provides genetically-engineered parvovirus capsids and viruses designed to introduce a heterologous gene into a target cell. The parvoviruses of the invention provide a repertoire of vectors with altered antigenic properties, packaging capabilities, and/or cellular tropisms as compared with current AAV vectors.
    Type: Grant
    Filed: July 26, 2002
    Date of Patent: February 6, 2007
    Assignee: University of North Carolina at Chapel Hill
    Inventors: Joseph E. Rabinowitz, Richard Jude Samulski, Weidong Xiao
  • Patent number: 6951753
    Abstract: A method for producing helper-free stocks of recombinant adeno-associated virus (rAAV) which can be used to efficiently and stably transduce foreign genes into host cells or organisms. The method comprises the cotransfection of eukaryotic cells with rAAV and with helper AAV DNA in the presence of helper virus (e.g. adenovirus or herpesvirus) such that the helper AAV DNA is not associated with virion formation. The crux of the invention lies in the inability of the helper AAV DNA to recombine with rAAV vector, thereby preventing the generation of wild-type virus. The pure stocks of recombinant AAV provide an AAV viral expression vector system with increased yield of recombinant virus, improved efficiency, higher definition, and greater safety than presently used systems.
    Type: Grant
    Filed: September 24, 2002
    Date of Patent: October 4, 2005
    Assignee: The 501 Trustees of Princeton University
    Inventors: Thomas E. Shenk, Richard Jude Samulski, Long-Sheng Chang
  • Patent number: 6951758
    Abstract: A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
    Type: Grant
    Filed: July 13, 2004
    Date of Patent: October 4, 2005
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Forrest K. Ferrari, Xiao Xiao, Richard Jude Samulski
  • Publication number: 20040259255
    Abstract: A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
    Type: Application
    Filed: July 13, 2004
    Publication date: December 23, 2004
    Applicant: University of North Carolina at Chapel Hill
    Inventors: Forest K. Ferrari, Xiao Xiao, Richard Jude Samulski
  • Patent number: 6703237
    Abstract: Primary receptors and co-receptors for adeno-associated virus (AAV) attachment to and infection of target cells are described. Such receptors can be used to facilitate AAV attachment to and infection of cells, e.g., for gene therapy. Methods for purification and/or concentration of AAV are also described. Methods of facilitating or enhancing AAV infection of a cell are also provided. Also described are methods of inhibiting or preventing infection of AAV into a cell. Cell samples may be screened for permissiveness for AAV attachment and infection by detecting the presence or abundance of cellular receptors that mediate attachment and/or infection of AAV into the cell. Formulations and kits for mediating AAV attachment to, and infection of, cells are also provided herein.
    Type: Grant
    Filed: March 20, 2002
    Date of Patent: March 9, 2004
    Assignee: University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Candace Summerford
  • Publication number: 20040029106
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Application
    Filed: January 21, 2003
    Publication date: February 12, 2004
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Publication number: 20040013645
    Abstract: The invention uses recombinant parvoviruses, and particularly recombinant adeno-associated virus (rAAV) to deliver genes and DNA sequences for gene therapy following manipulation of the therapeutic virus for packaging and transport. The invention delivers therapeutic viral vectors via rAAV affixed to support matrixes (i.e., sutures, surgically implantable materials, grafts, and the like).
    Type: Application
    Filed: December 19, 2002
    Publication date: January 22, 2004
    Inventors: Paul E. Monahan, Richard Jude Samulski, Joseph E. Rabinowitz
  • Patent number: 6670176
    Abstract: The subject invention concerns a recombinant adeno-associated virus vector characterized as being capable of delivering and expressing at least one mammalian gene into a genome of a mammalian host cell such that the expression of the gene is regulated in a tissue specific manner by cis-acting regulatory and promoter elements of the gene. A method of using this recombinant adeno-associated virus vector for therapeutic purposes is also provided.
    Type: Grant
    Filed: September 30, 1996
    Date of Patent: December 30, 2003
    Assignees: National Institutes of Health, University of Pittsburgh
    Inventors: Richard Jude Samulski, Christopher E. Walsh, Arthur W. Nienhuis, Johnson M. Liu, Jeffrey L. Miller