Abstract: High throughput and virtual screening methods are disclosed that can identify potential anti-viral agents. The virtual screening methods identify agents that interact with a viral nucleoprotein binding site. The high throughput methods identify compounds that inhibit viral infection by binding to viral nucleoprotein. Also disclosed are pharmaceutical formulations useful for treating or preventing viral infections, especially influenza A.

Abstract: Cell-based and cell-free assays are disclosed that detect compounds that promote aggregation of proteins, glycoproteins, and protein-nucleic acid complexes. Also disclosed are pharmaceutical formulations useful for treating or preventing viral infections, bacterial infections, cancer, and diseases involving hyper-proliferative cells.

Abstract: Compounds which exhibit antiviral activity, particularly against influenza virus, and methods of making and using thereof are described herein. In one embodiment, the compounds are heterocyclic amides containing piperazine and isozazole rings and optionally substituted with one or more substituents. The compounds can be formulated with one or more pharmaceutically acceptable excipients to form compositions suitable for enteral or parenteral administration. The compounds are preferably used to treat or prevent Influenza A infections, such as H1N1, H2N2, H3N2, H5N1, H7N7, H1N2, H9N2, H7N2, H7N3, and H10N7.

Abstract: Lead compounds were obtained in a high throughput screen (HTS) of angiogenin (ANG; a potent inducer of angiogenesis) enzyme activity, an RNase. One lead was shown to delay appearance of tumors in an animal tumor system, and to reduce the number of animals having tumors. Several lead compound analogs were found to be even more potent inhibitors of ANG activity compared to the original leads, and two were also found to have greater affinity for ANG than for pancreatic RNase. Other embodiments disclose a method comprising obtaining a ribonuclease inhibitor and a composition; and admixing the ribonuclease inhibitor and the composition to form an admixture, wherein a ribonuclease that may be present in the admixture is inhibited.

Abstract: The present application discloses an apparatus for gasification of solid, liquid and gaseous organic feed materials having a fuel value into synthesis gas. The apparatus comprises: a gasification reactor vessel having a tapered interior with a minimum of four zones for introducing reaction gas mixtures, a central perforated diffuser pipe, a solid and liquid material inlet at the upper end of the uppermost zone, a solid and gas discharge outlet at the lower end of the bottom zone, wherein the reactor vessel defining a sequence of reaction zones from the material inlet to the material outlet including drying, pyrolysis, combustion and gasification zones, and confining downwardly moving the column of the feed materials within the zones, gas feed inlet pipes for introducing reaction gas into the zones, a synthesis gas discharge pipe below the zones, and a heat exchanger below the zones.

Abstract: A novel driver circuit that uses a differential driver as a design backbone is described. Unlike a conventional differential interface, which typically has two or more outputs for providing an output signal and its complement, one of the differential driver's outputs is coupled to drive an output signal onto a signal line, while another one of the differential driver's outputs is unused and terminated, for instance by coupling the output to package ground or a voltage source via a capacitor. The performance of the driver circuit is significantly improved over conventional singled-ended driver designs.

Abstract: A novel driver circuit that uses a differential driver as a design backbone is described. Unlike a conventional differential interface, which typically has two or more outputs for providing an output signal and its complement, one of the differential driver's outputs is coupled to drive an output signal onto a signal line, while another one of the differential driver's outputs is unused and terminated, for instance by coupling the output to package ground or a voltage source via a capacitor. The performance of the driver circuit is significantly improved over conventional singled-ended driver designs.

Abstract: A novel driver circuit that uses a differential driver as a design backbone is described. Unlike a conventional differential interface, which typically has two or more outputs for providing an output signal and its complement, one of the differential driver's outputs is coupled to drive an output signal onto a signal line, while another one of the differential driver's outputs is unused and terminated, for instance by coupling the output to package ground or a voltage source via a capacitor. The performance of the driver circuit is significantly improved over conventional singled-ended driver designs.

Abstract: A novel driver circuit that uses a differential driver as a design backbone is described. Unlike a conventional differential interface, which typically has two or more outputs for providing an output signal and its complement, one of the differential driver's outputs is coupled to drive an output signal onto a signal line, while another one of the differential driver's outputs is unused and terminated, for instance by coupling the output to package ground or a voltage source via a capacitor. The performance of the driver circuit is significantly improved over conventional singled-ended driver designs.

Abstract: A waste-to-synthesis gas system including: a first gasifier for receiving biomass; a gas distributor for delivering reactant gas and oxygen into the first gasifier in a countercurrent direction to the biomass flow and to define a plurality of reaction regions including a drying region, a pyrolysis region, a gasification region and a combustion region; and, a second gasifier for receiving gases from the plurality of regions of the first gasifier and a gas distributor for delivering reactant gas and oxygen into the second gasifier in a concurrent direction to the flow of gases from the first gasifier. As a result, no carbon chars, oils or tars are expected to be present in the synthesis gas produced.

Abstract: The present invention concerns a method for detecting the presence of a ribosome inactivating protein in a sample. The method comprises contacting a sample suspected of containing a ribosome inactivating protein with a 2?-O-methylated oligonucleotide substrate having a GAxGA tetraloop wherein “Ax” is a deoxyribonucleoside comprising a fluorescent adenine derivative or analog base capable of emitting a fluorescence when released from the nucleoside, “Ax”, and detecting the presence of the fluorescent adenine derivative or analog base released from the tetraloop as an indication of the presence of the ribosome inactivating protein in the sample. The present invention is also directed to an assay kit and a reagent useful for carrying out the steps of the above method.

Abstract: A method for propagating a business event is provided. The method includes defining an event, registering a source for the event and registering a component interested in the event. The method also includes defining a data channel to be used for a notification of the event. The event is identified and the notification of the event is transmitted to the interested component via the data channel. A system for implementing the method is also provided.

Abstract: The storage stability of peroxidase-reactive luminescent cocktails which employ chemiluminescent 2,3-dihydro-1,4-phthalazinediones, oxidants reactant therewith to cause light emission in peroxidase-mediated luminescent reactions, and phenolic sensitivity enhancers is improved by maintaining the pH of the cocktail prior to use at a value in the range of about 3 to about 6 and preferably at a pH of about 5. An analyte sample may be subjected to a reaction resulting in a peroxidase-containing phase, the peroxidase content of which is related to the quantity of analyte in the sample, and the peroxidase phase may then be combined with the thus-described cocktail and an aqueous alkaline organic buffer to raise the pH of the resulting reaction mixture to a value in the range of 7-9, favoring light emission.