Abstract: The present invention relates to a new cassette for the expression of an endothiapepsin precursor in Cryphonectria parasitica, to a strain of this species transformed with this cassette, to a process for preparing endothiapepsin using this strain and also to a process for preparing such a strain devoid of a dominant selection marker.

Abstract: The invention relates to a method for the extraction of recombinant periplasmic proteins wherein arginine is used as the extraction agent. In particular, the invention relates to a method for the extraction of a periplasmic protein of interest, which essentially consists in:(i) suspending the pellet of cells or of cell debris from cells, which cells originate from the culture of a prokaryotic microorganism transformed with an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and(ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.

Abstract: The invention relates to a DNA fragment of sequence S.sub.1 as described in the application and encoding the S.sub.2 peptide sequence as described in the application. This DNA sequence carries the gene encoding the enzyme for fragmenting N-acetylheparosan and the adjacent sequences permitting its expression. The invention also relates to an enzyme intended for fragmenting N-acetylheparosan derived from this gene and the processes for fragmenting high molecular mass N-acetylheparosan using this enzyme.

Abstract: The invention relates to a method for the extraction of recombinant periplasmic proteins wherein arginine is used as the extraction agent. In particular, the invention relates to a method for the extraction of a periplasmic protein of interest, which essentially consists in:(i) suspending the pellet of cells or of cell debris from cells, which cells originate from the culture of a prokaryotic microorganism transformed with an expression vector containing a gene coding for the protein and means for its expression in the periplasm of the microorganism, in a buffer solution containing arginine; and(ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.

Abstract: The invention concerns a new urate oxidase activity protein which has the following sequence (SEQ ID NO:1): ##STR1## optionally preceded by a methionine, or in that it may present a degree of substantial homology with this sequence.The invention is also aimed at medicines containing this protein as well as the genetic engineering implements to obtain it.

Abstract: The invention relates to a DNA fragment of SEQ ID NO: 1 herein and encoding the SEQ ID NO: 2 peptide sequence herein. This DNA sequence carries the gene encoding the enzyme for fragmenting N-acetylheparosan and the adjacent sequences permitting its expression. The invention also relates to an enzyme intended for fragmenting N-acetylheparosan derived from this gene and the processes for fragmenting high molecular mass N-acetylheparosan using this enzyme.

Abstract: Recombinant DNA which codes for a protein with endochitinase activity or for a precursor thereof, comprising the sequence (a1) (SEQ ID NO. 1).

Abstract: The invention concerns a new urate oxidase activity protein which has the following sequence:______________________________________ Ser Ala Val Lys Ala Ala Arg Tyr Gly Lys Asp Asn Val Arg Val Tyr Lys Val His Lys Asp Glu Lys Thr Gly Val Gln Thr Val Tyr Glu Met Thr Val Cys Val Leu Leu Glu Gly Glu Ile Glu Thr Ser Tyr Thr Lys Ala Asp Asn Ser Val Ile Val Ala Thr Asp Ser Ile Lys Asn Thr Ile Tyr Ile Thr Ala Lys Gln Asn Pro Val Thr Pro Pro Glu Leu Phe Gly Ser Ile Leu Gly Thr His Phe Ile Glu Lys Tyr Asn His Ile His Ala Ala His Val Asn Ile Val Cys His Arg Trp Thr Arg Met Asp Ile Asp Gly Lys Pro His Pro His Ser Phe Ile Arg Asp Ser Glu Glu Lys Arg Asn Val Gln Val Asp Val Val Glu Gly Lys Gly Ile Asp Ile Lys Ser Ser Leu Ser Gly Leu Thr Val Leu Lys Ser Thr Asn Ser Gln Phe Trp Gly Phe Leu Arg Asp Glu Tyr Thr Thr Leu Lys Glu Thr Trp Asp Arg Ile Leu Ser Thr Asp Val Asp Ala Thr Trp Gln Trp Lys Asn Phe Ser Gly Leu Gln Glu Val Arg Ser His Val Pro Lys Phe Asp Ala Thr Trp Ala Thr Ala Arg Glu Val Thr Leu Lys Thr Phe Ala Glu Asp Asn

Abstract: The invention relates to a signal peptide of the formula:MXKSTLLLLFLLLCLPSWNAGAand X represents a direct bond between M and K, an amino acid selected from the group comprising the 20 amino acids of the genetic code, or a peptide containing 2, 3 or 4 amino acids selected, each independently of the other, from the group comprising the 20 amino acids of the genetic code.

Abstract: DNA sequence participating in the regulation of the expression of a DNA sequence coding for a precursor of a polypeptide.Expression vectors containing such a sequence.Process for the periplasmic production of a polypeptide in a strain of Gram-negative bacteria transformed by one of these vectors.Application to the production of human growth hormone.

Abstract: The invention relates to a microbiological process for the preparation of a protein by culturing a mutant bacterial strain.This process consists in using a bacterial strain carrying a mutation which limits, or even suppresses, the expression of the operons whose transcription depends on the fixation of the cAMP-CRP complex, a DNA sequence coding for a protein precursor having been introduced into the said bacterial strain.