Patents by Inventor Richard Mathies

Richard Mathies has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9969970
    Abstract: The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.
    Type: Grant
    Filed: March 1, 2016
    Date of Patent: May 15, 2018
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Shih-Chia Hsiao, Matthew B. Francis, Carolyn Bertozzi, Richard Mathies, Ravi Chandra, Erik Douglas, Amy Twite, Nicholas Toriello, Hiroaki Onoe
  • Patent number: 9835624
    Abstract: The present disclosure provides methods of detecting mycobacterium in an individual, generally involving detecting antibody to a mycobacterial lipid in a biological sample obtained from the individual. The present disclosure further provides compositions and kits for carrying out the methods.
    Type: Grant
    Filed: April 16, 2012
    Date of Patent: December 5, 2017
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Lee W. Riley, Richard A. Mathies, Amador Goodridge, Jungkyu Kim, Robert Eugene Snyder
  • Patent number: 9791068
    Abstract: The present disclosure relates to method, system for microfluidic control. One or more embodiments of the disclosure relate to pneumatically actuated “lifting gate” microvalves and pumps. In some embodiments, a microfluidic control module is provided, which comprises a plurality of pneumatic channels and a plurality of lifting gate valves configured to be detachably affixed to a substrate. The plurality of lifting gate valves are aligned with at least one fluidic channel on the substrate when affixed to the substrate. Each of the valves comprises: a pneumatic layer, a fluidic layer, and a pneumatic displacement chamber between the pneumatic layer and the fluidic layer. The fluidic layer has a first side facing the pneumatic layer and a second side facing away from the pneumatic layer, wherein the second side has a protruding gate configured to obstruct a flow of the fluidic channel when the fluidic layer is at a resting state.
    Type: Grant
    Filed: January 14, 2014
    Date of Patent: October 17, 2017
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Jungkyu Kim, Erik C. Jensen
  • Patent number: 9651039
    Abstract: Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.
    Type: Grant
    Filed: September 3, 2008
    Date of Patent: May 16, 2017
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, William H. Grover, Alison Skelley, Eric Lagally, Chung N. Liu
  • Patent number: 9644623
    Abstract: Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.
    Type: Grant
    Filed: May 18, 2010
    Date of Patent: May 9, 2017
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, William H. Grover, Alison Skelley, Eric Lagally, Chung N. Liu
  • Publication number: 20160319238
    Abstract: The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.
    Type: Application
    Filed: March 1, 2016
    Publication date: November 3, 2016
    Inventors: Shih-Chia Hsiao, Matthew B. Francis, Carolyn Bertozzi, Richard Mathies, Ravi Chandra, Erik Douglas, Amy Twite, Nicholas Toriello, Hiroaki Onoe
  • Publication number: 20160265043
    Abstract: A short tandem repeat (STR) typing method and system are developed for forensic identification of individual cells. Agarose-in-oil droplets are produced with a high frequency using a microfluidic droplet generator. Statistically dilute single cells, along with primer-functionalized microbeads, are randomly compartmentalized in the droplets. Massively parallel single-cell droplet PCR is performed to transfer replicas of desired STR targets from the single-cell genomic DNA onto a coencapsulated microbead. These DNA-conjugated beads are subsequently harvested and reamplified under statistically dilute conditions for conventional capillary electrophoresis STR fragment size analysis. The methods and systems described herein are valuable for the STR analysis of samples containing mixtures of cells/DNA from multiple contributors and for low concentration samples.
    Type: Application
    Filed: November 5, 2014
    Publication date: September 15, 2016
    Inventors: Tao Geng, Richard Novak, Richard A. Mathies
  • Patent number: 9327036
    Abstract: The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.
    Type: Grant
    Filed: April 8, 2010
    Date of Patent: May 3, 2016
    Assignee: The Regents of the University of California
    Inventors: Shih-Chia Hsiao, Matthew B. Francis, Carolyn Bertozzi, Richard Mathies, Ravi Chandra, Erik Douglas, Amy Twite, Nicholas Toriello, Hiroaki Onoe
  • Patent number: 8841116
    Abstract: Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.
    Type: Grant
    Filed: October 25, 2007
    Date of Patent: September 23, 2014
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Robert Blazej, Palani Kumaresan
  • Publication number: 20140197339
    Abstract: The present disclosure relates to method, system for microfluidic control. One or more embodiments of the disclosure relate to pneumatically actuated “lifting gate” microvalves and pumps. In some embodiments, a microfluidic control module is provided, which comprises a plurality of pneumatic channels and a plurality of lifting gate valves configured to be detachably affixed to a substrate. The plurality of lifting gate valves are aligned with at least one fluidic channel on the substrate when affixed to the substrate. Each of the valves comprises: a pneumatic layer, a fluidic layer, and a pneumatic displacement chamber between the pneumatic layer and the fluidic layer. The fluidic layer has a first side facing the pneumatic layer and a second side facing away from the pneumatic layer, wherein the second side has a protruding gate configured to obstruct a flow of the fluidic channel when the fluidic layer is at a resting state.
    Type: Application
    Filed: January 14, 2014
    Publication date: July 17, 2014
    Inventors: Richard A. Mathies, Jungkyu Kim, Erik C. Jensen
  • Publication number: 20140162283
    Abstract: The present disclosure provides methods of detecting mycobacterium in an individual, generally involving detecting antibody to a mycobacterial lipid in a biological sample obtained from the individual. The present disclosure further provides compositions and kits for carrying out the methods.
    Type: Application
    Filed: April 16, 2012
    Publication date: June 12, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Lee W. Riley, Richard A. Mathies, Amador Goodridge, Jungkyu Kim, Robert Eugene Snyder
  • Patent number: 8454906
    Abstract: Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: June 4, 2013
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Palani Kumaresan, Chaoyang Yang, Robert G. Blazej
  • Patent number: 8420318
    Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: April 16, 2013
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Robert Blazej, Chung Liu, Palani Kumaresan, Stephanie H. I. Yeung
  • Patent number: 8286665
    Abstract: Membrane valves and latching valve structures for microfluidic devices are provided. A demultiplexer can be used to address the latching valve structures. The membrane valves and latching valve structures may be used to form pneumatic logic circuits, including processors.
    Type: Grant
    Filed: June 18, 2010
    Date of Patent: October 16, 2012
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, William H. Grover, Erik C. Jensen
  • Publication number: 20120142088
    Abstract: The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.
    Type: Application
    Filed: April 8, 2010
    Publication date: June 7, 2012
    Applicant: The Regents of the University of California
    Inventors: Shih-Chia Hsiao, Matthew B. Francis, Carolyn Bertozzi, Richard Mathies, Ravi Chandra, Erik Douglas, Amy Twite, Nicholas Toriello, Hiroaki Onoe
  • Publication number: 20120142010
    Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.
    Type: Application
    Filed: February 13, 2012
    Publication date: June 7, 2012
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Richard A. MATHIES, Robert BLAZEJ, Chung LIU, Palani KUMARESAN, Stephanie H.I. YEUNG
  • Publication number: 20110020920
    Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.
    Type: Application
    Filed: July 27, 2010
    Publication date: January 27, 2011
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Richard A. MATHIES, Robert BLAZEJ, Chung LIU, Palani KUMARESAN, Stephanie H. I. YEUNG
  • Publication number: 20100285975
    Abstract: Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with.
    Type: Application
    Filed: July 24, 2008
    Publication date: November 11, 2010
    Applicant: The Regents of the University of California
    Inventors: Richard A. Mathies, Palani Kumaresan, Chaoyang Yang, Robert G. Blazej
  • Publication number: 20100252123
    Abstract: Membrane valves and latching valve structures for microfluidic devices are provided. A demultiplexer can be used to address the latching valve structures. The membrane valves and latching valve structures may be used to form pneumatic logic circuits, including processors.
    Type: Application
    Filed: June 18, 2010
    Publication date: October 7, 2010
    Applicant: The Regents of the University of California
    Inventors: Richard A. Mathies, William H. Grover, Erik C. Jensen
  • Patent number: 7799553
    Abstract: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments.
    Type: Grant
    Filed: May 25, 2005
    Date of Patent: September 21, 2010
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Robert Blazej, Chung Liu, Palani Kumaresan, Stephanie H. I. Yeung