Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

Abstract: An apparatus for detecting analytes in a sample is provided. The apparatus can include: a flow cytometry system including one or more detection zones; one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from detectable analytes in the one or more detection zones, which have been excited by the radiation, wherein at least one detector includes an output; a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector; and a sorting mechanism, for example, to effect flow cytometry. Methods for detecting analytes in a sample are also provided.

Abstract: Apparatus and methods for detecting analytes in a sample are provided. The apparatus can include: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with radiation; a detector capable of collecting at least one charge corresponding to an emission beam emitted from the detection zone, the detector having an output; and a time delay integration system coupled to the detector for effecting time delay integration of the at least one charge by accumulating the at least one charge before reading the at least one charge at the output of the detector. Various embodiments provide an apparatus with a multi-notch filter. Various embodiments provide a solid state laser, a micro-wire laser, or an organic light-emitting diode as a irradiation source.

Abstract: Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Abstract: An optical system for analyzing light from a plurality of samples is provided. The optical system includes a plurality of holders adapted to have samples located therein, a collection lens, a transmission grating, and a reimaging lens. The collection lens is configured to receive and substantially collimate light from the samples. The transmission grating is configured to spectrally disperse the substantially collimated light from the collection lens. The reimaging lens is configured to receive the light from the light dispersing element and direct the light onto a light detection device. A method of optically analyzing at least one sample is also provided.

Abstract: A method for correcting cartridge color shifts for imaging with an imaging apparatus includes determining a spectral signature band for each colorant to yield a plurality of spectral signature bands; determining a minimum number of the spectral signature bands from the plurality of spectral signature bands to form a group of spectral signature bands that are applicable to all colorants of the plurality of colorants; determining a primary colorant profile for each colorant of the plurality of colorants of the cartridge based on the minimum number of the spectral signature bands; determining primary colorant shift data based on the primary colorant profile for each colorant and based on a standard cartridge primary colorant profile; estimating combined colorant shift data using the primary colorant shift data; and generating color correction data based on the combined colorant shift data for use in imaging with an imaging apparatus using the cartridge.

Abstract: A method of selecting inks for use in imaging with an imaging apparatus includes establishing a high fidelity colorant cube having a plurality of vertex lines pertaining to a corresponding plurality of basic inks, and at least one other vertex line pertaining to at least one high fidelity ink; determining first mixing coordinates of the at least one high fidelity ink and the plurality of basic inks for face grids of the high fidelity colorant cube based on a maximum permitted ink amount; determining second mixing coordinates of the at least one high fidelity ink and the plurality of basic inks for interior grids of the high fidelity colorant cube based on the maximum permitted ink amount; and generating a color conversion lookup table based on the first mixing coordinates and the second mixing coordinates for printing with the imaging apparatus.

Abstract: Various embodiments described in the application relate to an apparatus, system, and method for fluidically controlling, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise combinations of detectors, controllers, valves, and fluid supply units to control the spatial size, location and/or fluidic composition of discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

Abstract: Various embodiments relate to systems and/or methods for sample preparation that can be used for biochemical and/or molecular biology procedures involving small volumes, for example, micro volumes or smaller. Methods and systems that can reduce sample size requirements and increase the number of samples on a substrate are provided. Samples can be applied to a plate or other appropriate substrate and can be used for, inter alia, sequencing reactions. In some embodiments, apparatuses, systems, and/or methods for charged analyte collection are provided. Charged analytes in a sample can be electrokinetically collected or extracted from a conduit through a hole formed in a sidewall of the conduit, by application of an electric field that causes the charged analytes to migrate in a direction that is transverse to the conduit.

Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.

Abstract: A method of correcting color shift caused by printing with an imaging system using a first cartridge and a second cartridge different from the first cartridge includes determining individual cartridge characterization data based on each of the first cartridge and the second cartridge individually; determining combined cartridge characterization data that characterizes a usage combination of the first cartridge and the second cartridge; and determining color correction data based on the individual cartridge characterization data and the combined cartridge characterization data.

Abstract: Embodiments of methods and devices are disclosed for the manipulation (e.g., concentration, purification, capture, trapping, location, transfer) of analytes, e.g., biomolecules, with respect to analyte-containing solutions, using one or more electric fields.

Abstract: A method of selecting inks for use in imaging with an imaging apparatus includes determining a maximum usage of a diluted ink for use in conjunction with a saturated ink based on visual perception characteristics relating to a combination of the diluted ink and the saturated ink; generating an initial colorant space based on the maximum usage of the diluted ink, the initial colorant space expressing an initial usage of the diluted ink and an initial usage of the saturated ink at each point in the initial colorant space; optimizing the initial usage of the diluted ink and the initial usage of the saturated ink in the initial colorant space to generate a final usage of the diluted ink and a final usage of the saturated ink in a final colorant space; and generating a color conversion lookup table based on the final colorant space.

Abstract: A method for converting a source gamut of an image to a destination gamut includes providing an imaging apparatus having a plurality of destination gamuts, determining a source gamut boundary for the source gamut of the image; selecting an appropriate destination gamut from the plurality of destination gamuts based on a desired output; mapping to the appropriate destination gamut based at least in part on the source gamut boundary; and determining a color conversion lookup table based on the mapping to the appropriate destination gamut for use in replicating the image to provide the desired output using the imaging apparatus.

Abstract: A method of printing using a plurality of basic colorants and at least one high fidelity colorant includes obtaining system variables that impact high fidelity color printing; and printing with an optimized usage of the plurality of basic colorants and the at least one high fidelity colorant based on a colorant level, wherein a reduced output of the at least one high fidelity colorant will occur if an average colorant level of the at least one high fidelity colorant is lower than an average colorant level of the plurality of basic colorants.

Abstract: A method for correcting neutral color shift in a production cartridge for use in an imaging system includes determining a colorant increment data based on a standard cartridge neutral variation signature color data and a standard cartridge neutral sensitivity signature color data associated with a standard cartridge, and based on a production cartridge neutral variation signature color data and a production cartridge neutral sensitivity signature color data associated with the production cartridge, and using the colorant increment data to correct the neutral color shift.

Abstract: An optical system for analyzing light from a plurality of samples is provided. The optical system includes a plurality of holders adapted to have samples located therein, a collection lens, a transmission grating, and a reimaging lens. The collection lens is configured to receive and substantially collimate light from the samples. The transmission grating is configured to spectrally disperse the substantially collimated light from the collection lens. The reimaging lens is configured to receive the light from the light dispersing element and direct the light onto a light detection device. A method of optically analyzing at least one sample is also provided.

Abstract: An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted from markers in the detection zone excited by the radiation, the detector array having an output; and a system coupled to the detector array for effecting time delay integration of the charges on the detector array corresponding to the light signals by accumulating the charges before reading the charges at the output of the detector array. Other apparatus and methods for detecting analytes in a sample are also provided.