Abstract: A capillary 1 is moved to a position above a vessel 17 having a solution 15 stored therein. Then, the vessel 17 is raised while the inside of a tube 5 is opened to an ambient pressure until one end 1a of the capillary 1 is dipped into the solution 15. The solution 15 is introduced into the capillary 1 by means of capillarity. Next, the vessel 17 is lowered, to thereby removing the end 1a of the capillary 1 from the solution 15 stored in the vessel 17. Subsequently, the capillary 1 is moved to a position above a vessel 19 to which the solution 15 is to be transferred. Then, the tube 5 is connected to a pressure mechanism, thereby pressurizing the inside of the capillary 1 from another end 1b thereof. The solution 15 in the capillary 1 is then discharged to the vessel 19.

Abstract: A holder for a capillary cassette closes a chamber, and is fixed to a detection side holder fixing member. An acidic solution container, an alkaline solution container, a pure water container and a drain container are arranged on a reservoir stage having a dry chamber. A holder up/down mechanism and a stage moving mechanism successively bring an end of a capillary array into contact with an acidic solution, an alkaline solution, pure water and nitrogen gas, and the chamber is decompressed for successively introducing these into capillary columns and performing pretreatment. Thereafter a gel container is arranged in the chamber, which in turn is pressurized for charging the capillary columns with a gel.

Abstract: A table is used to position eight specimen reservoirs of an electrophoretic chip into which a specimen is firstly dispensed to a specimen dispensing position. A specimen dispensing mechanism is used to move a head to thereby suck a test-specimen contained in eight different wells of specimen plates into eight nozzles respectively and then move the head to the specimen dispensing position, thus dispensing the test-specimen sucked into the nozzles into the specimen reservoirs simultaneously. The table is used to sequentially position the specimen reservoirs to the specimen dispensing position and then the specimen dispensing mechanism is used to sequentially dispensing the specimen into the specimen reservoirs.

Abstract: The present invention relates to a method for peptide C-terminal fragment sequence analysis, in which the fragment collection is carried out on an allylamine group-derivatized polymer membrane or on allylamine group-derivatized glass fiber filter paper; the collected C-terminal fragment is immobilized thereon using a water-soluble carbodiimide etc.; and the obtained immobilized product is subjected directly to amino acid sequence analysis. The present invention also relates to an apparatus for collecting a peptide fragment. According to the method of the present invention, peptides which are rich in hydrophobic groups in their C-terminus and are therefore difficult to trap with polyvalent ion carriers, currently used in the gas-phase sequencer, can be completely analyzed up to their C-terminus. Also, amino acid sequence analysis can be made even when the amount of C-terminal fragments is very small.

Abstract: The present invention relates to an apparatus for collecting a peptide fragment, wherein a C-terminal peptide fragment is collected from a peptide fragment mixture resulting from specific cleavage of the peptide bond between a lysine residue and the C-terminal amino acid residue adjacent thereto. The apparatus has an immobilizing means, a cleaving means, a recovering means and a control means. According to the present invention, since the control means sequentially executes the immobilizing means, cleaving means and recovering means, no skillful work is required in peptide fragment collection, making it possible to collect the carboxyl-terminal peptide fragment with simple operation and high reproducibility.

Abstract: The present invention is directed to a simultaneous multiple chemical synthesizer comprising a number of reaction vessels wherein each vessel has a filter in the bottom portion thereof, a number of needles wherein each needle is connected to an aspiration injection line of a reaction mixture and a gas supply line in connection with each reaction vessel and each needle does not touch the resin contained in the reaction vessel, a number of arms which are horizontally and vertically movable and hold the respective needles, a bubbling gas line and a waste discharge line in connection with each reaction vessel, wherein each line is connected to the bottom portion of each reaction vessel, a number of purging means which move synchronously with said waste discharge lines, and means for washing the portions of the needles and purge means which contact with the reaction reagents.

Abstract: In connection with an automated peptide synthesizer, an apparatus and associated method through which peptides produced in simultaneous multiple synthesis are together isolable. Cleavage to liberate the peptides from linkage to the support matrix of the solid phase is carried out in the same vessels in which the synthesizing reactions took place. Prior to cleavage, a drainage port of each reaction vessel is closed off by a cap. The reaction vessel is inserted into a centrifuge tube, which in turn is put into a rack supporting a number of tubes equal to the number of channels of the peptide synthesizing apparatus. After the peptides are cleaved and dissolved into cleaving solution added into each of the reaction vessels, the caps are removed from the drainage ports of each reaction vessel, and the vessel is returned into the centrifuge tube. A plastic jet-fitting attached to the nozzle tip of a blow unit pressure gun is inserted into and pressed into contact against the supply opening of the reaction vessel.

Abstract: In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains.