Abstract: Methods for preparing strand-specific sequencing libraries of oligonucleotides using an RNA polymerase promoter to re-transcribe antisense cDNA which has been reverse transcribed from mRNA are provided. The transcription step linearly amplifies sRNA prior to production of double-stranded cDNA to be sequenced and may be sufficient to eliminate the conventional PCR amplification step prior to sequencing. The methods incorporate anchor sequences, amplification sequences and other sequences required for a particular sequencing system or reaction by hybridization and extension of primers, and transcription of RNA, rather than ligation, thus reducing the number of steps and the time required for sample preparation for sequencing of RNA. Use of primer hybridization and transcription reactions in the methods also results in a library that exhibits reduced 3? sequence bias.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods and kits are provided for the production and use in microarray assays of labeled miRNA molecules and labeled cDNA molecules complementary to miRNA molecules.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods, reagents and kits are provided for the production and use in detection assays of labeled nucleic acid molecules wherein a labeling molecule is attached directly to the 3? end of the nucleic acid molecules.

Abstract: Methods and kits are provided for performing multiple rounds of sense RNA synthesis. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules are prepared by: providing a single stranded cDNA molecule having 5? and 3? ends; attaching an oligodeoxynucleotide tail to the 3? end of said single stranded cDNA molecule; providing a double stranded RNA polymerase promoter having a sense strand and antisense strand, wherein the sense strand comprises a single stranded 3? overhang comprising a sequence complementary to said oligodeoxynucleotide tail; annealing said double stranded RNA polymerase promoter to said oligodeoxynucleotide tail by complementary base pairing with said 3? overhang sequence; ligating the 5? end of the antisense strand of said double stranded RNA polymerase promoter to the 3? end of said oligodeoxynucleotide tail; and initiating RNA transcription using an RNA polymerase which recognizes said double stranded promoter, thus producing a sense RNA molecule (sRNA).

Abstract: Methods, reagents and kits are provided for the production and use in detection assays of labeled nucleic acid molecules wherein a labeling molecule is attached directly to the 3? end of the nucleic acid molecules.

Abstract: Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

Abstract: Methods for detecting nucleic acids that do not require incorporation of labeled nucleotides into the hybridized strand of either the target or the probe nucleic acid. A nucleic acid is detected by adding a capture sequence onto the end of the single stranded probe or target and then hybridizing that capture sequence to a complementary sequence on a signal carrying molecule. Alternatively, a nucleic acid is detected by using an existing sequence on one of the two unlabeled single strands as the capture sequence, and then hybridizing that sequence to a complementary sequence of a signal carrying molecule. In preferred embodiments, the signal carrying molecule is a dendrimer.

Abstract: A composition for forming a protective coating over the surface of an assay substrate having an indicating agent capable of generating a detectable signal associated therewith, includes a protective coating forming material, and a delivery system for delivering the protective coating forming material in an amount sufficient to coat the surface of the assay substrate, wherein the delivery system evaporates from the surface of the assay substrate to form a protective coating at least substantially composed of the protective coating forming material that is at least substantially transparent to the detectable signal generated by the indicating agent. The present invention is further directed to a method for forming the protective coating over the surface of the assay substrate, and a substrate produced by such method.

Abstract: Methods are provided for blocking non-specific and specific hybridization of nucleic acid samples on a microarray. The method of the present invention comprises applying a blocking reagent to the microarray, wherein the blocking reagent comprises modified nucleotide bases, preferably LNA (Locked Nucleic Acid—modified bicyclic monomeric units with a 2′-O-4′-C methylene bridge). In further embodiments, the method includes applying a mixture including: a) a cDNA reagent obtained from mRNA of a target sample, the cDNA having a capture sequence; b) a dendrimer with a label for emitting a detectable signal and a second nucleotide sequence complementary to the capture sequence; and c) an blocking reagent containing LNA to a microarray, for producing a detectable signal from said label whereby a hybridization pattern is generated on the microarray.

Abstract: Disclosed are reagents for conducting quality control reactions on microarrays of nucleic acids and kits containing the reagents, along with directions for conducting the reactions with the components in the kits. Also disclosed are methods of preparing the kits as well as using them to conduct the quality control reactions. A preferred reagent embodiment is illustrated in FIG. 3.

Abstract: The present invention is directed to a method for determining the presence of a specific nucleotide sequence in a cDNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the cDNA reagent on a microarray through temperature cycling.

Abstract: The present invention is directed to a method for determining the presence of a specific nucleotide sequence in an RNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the RNA reagent on a microarray.