Abstract: Disclosed is a computer-implemented method of rebalancing persistent client connections to a cluster of servers. The method comprises identifying an increase in a total client connection capacity of the cluster of servers with a network connection balancing component; and for each server in a selection of servers in the cluster of servers calculating a current client connection capacity utilization of the server from the number of persistent connections to the server and the current capacity of the server; comparing the current client connection capacity utilization with the target client connection capacity utilization; and terminating a selection of its persistent client connections by a server based on its current client connection capacity utilization exceeding the target client connection capacity utilization. Also disclosed are a computer program product and a computer system for utilizing the computer-implemented method.

Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5? to the target sequence, and a second probe-binding sequence 3? to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

Abstract: Described herein are food formulations and methods, which promote sleep.

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5? to the target sequence, and a second probe-binding sequence 3? to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

Abstract: Methods and apparatus for tilting and filling a carton with a pouch and a flowable food product are described. The carton can be tilted prior to filling in order to minimize a void between the pouch and a sidewall of the carton. The carton is pivoted to a tilted orientation, such that the sidewall includes a lowermost inclined segment. The pouch is then inserted into the carton either prior to or after pivoting the carton to the tilted orientation so that the pouch can substantially rest against the inclined segment. The flowable food product is then at partially deposited into the carton, such that the flowable food product substantially fills in next to the pouch instead of between the pouch and the inclined segment. After a sufficient height of flowable food product has been deposited into the carton, the carton can be returned to a vertical orientation and closed.

Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein.

Abstract: Method and system are provided for automatic failover between multiple nodes in a middle-tier layer between client applications and a back-end service. The method at a first node includes: receiving a request from a client application; determining that the first node cannot service the request successfully; forwarding the request from the first node to a second peer node; receiving a response at the first node on completion of the request by the second peer node; and forwarding the response from the first node to the client application. An original path of communication between the client application and the first node is maintained. The failover of the request from the first node to the second peer node is transparent to the client application. Nodes may be registered in a group, wherein a node in a group has awareness of other nodes in the group.

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract: A method, system, and computer programming product for returning a response directly to a transaction request originator in a customer information control system (CICS) is provided. The method can include receiving a transaction request in a CICS and allocating resources in the CICS in response to receiving the transaction request. The method additionally can include determining whether the CICS is an intended endpoint of the transaction request. Finally, the method can include extracting an address of an originator of the transaction request from the transaction request and sending a response to the originator at the extracted address upon determining that the CICS is the intended endpoint of the transaction request, but otherwise routing the transaction request to a different CICS along with the address within the transaction request.

Abstract: Embodiments of systems, devices, and methods for treating, remediating, or abating carbon-containing wastes generate at least one of clean water; non-toxic, non-hazardous ash; or power. Some embodiments are modular, permitting rapid deployment, flexible configuration, and easy transportation. Embodiments of the systems treat carbon-containing aqueous waste, carbon containing waste, or a combination thereof. The systems, devices, and methods are particularly suited to treating hydrocarbon containing waste generated in oil and natural gas drilling and hydrofracturing.

Abstract: Described herein are food formulations and methods, which promote sleep.

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

Abstract: Method and system are provided for automatic failover between multiple nodes in a middle-tier layer between client applications and a back-end service. The method at a first node includes: receiving a request from a client application; determining that the first node cannot service the request successfully; forwarding the request from the first node to a second peer node; receiving a response at the first node on completion of the request by the second peer node; and forwarding the response from the first node to the client application. An original path of communication between the client application and the first node is maintained. The failover of the request from the first node to the second peer node is transparent to the client application. Nodes may be registered in a group, wherein a node in a group has awareness of other nodes in the group.

Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

Abstract: A system and computer program product for assigning runtime artifacts to software components. Source code and metadata are extracted from a library, where the metadata includes a component membership for the source code. A compile unit is formed from the extracted source code and metadata. Runtime artifacts are produced from the compile unit or an element derived from the compile unit. Each runtime artifact is interrogated to determine the defined component for each runtime artifact. The determined component is recorded.

Abstract: A method for assigning runtime artifacts to software components. Source code and metadata are extracted from a library, where the metadata includes a component membership for the source code. A compile unit is formed from the extracted source code and metadata. Runtime artifacts are produced from the compile unit or an element derived from the compile unit. Each runtime artifact is interrogated to determine the defined component for each runtime artifact. The determined component is recorded.

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.