Abstract: A tissue retraction system includes a dilator and a first retractor member and a retraction assembly. The dilator is configured to be inserted into a tissue body and also includes at least one engagement member. The first retractor member includes a body and at least one engagement member that is configured to attach to the at least one engagement member of the dilator so as to removably attach the retractor member to the dilator body. The retraction assembly includes a retractor body and at least a second retractor member that is movably supported by the retractor body. The retractor body is configured to be attached to the first retractor member. The tissue protection system may also include a neuromonitoring member configured to determine a characteristics of the tissue.

Abstract: A dilator assembly includes a dilator and a retractor member that is configured to be removably attached to the dilator.

Abstract: Methods and compositions for the expression of nucleotide sequences are provided. Compositions comprise the nucleic acid sequences of the transcriptional regulatory region of the PpSEC10 gene of Pichia pastoris. Methods are provided to chemically regulate the PpSEC10 transcriptional control region by modulating the iron concentration in the culturing medium. The methods find use in regulating expression of nucleotide sequences. Furthermore, the methods of the invention can be used to regulate polypeptide expression, more particularly in regulating heterologous polypeptide expression, particularly using a yeast host cell as the expression system.

Abstract: Polynucleotides, vectors and host cells comprising a polynucleotide having a fragment of a leader sequence and a second nucleotide sequence that encodes a polypeptide heterologous to the leader sequence, wherein the leader sequence fragment is sufficient for secretion and comprises an amino acid sequence that comprises at least about 70% sequence identity to the leader sequence of Pichia acaciae killer toxin, wherein the heterologous polypeptide is not naturally contiguous to the leader sequence, and wherein upon expression of the polynucleotide molecule in a host cell suitable for expression thereof, the heterologous polypeptide is produced that is free of additional N-terminal amino acids.

Abstract: Polynucleotides, vectors, and host cells comprising a polynucleotide having a fragment of a leader sequence and a second nucleotide sequence that encodes a polypeptide heterologous to the leader sequence, wherein the leader sequence fragment is sufficient for secretion and comprises an amino acid sequence that comprises at least about 70% sequence identity to the leader sequence of Pichia acaciae killer toxin, wherein the heterologous polypeptide is not naturally contiguous to the leader sequence, and wherein upon expression of the polynucleotide molecule in a host cell suitable for expression thereof, the heterologous polypeptide is produced that is free of additional N-terminal amino acids.

Abstract: A dynamic reef is provided in accordance with the invention. The dynamic reef includes anchoring means for anchoring the reef in a substantially fixed position with respect to a shoreline, an elastic rubber tubing secured to the anchoring means, and at least one flotation collar positioned so as to be rotatable about the rubber tubing for the purpose of dissipating wave energy. A solar strobe unit may be positioned on the rubber tubing at the end opposite the anchoring means and functions as a warning device. A method for preventing shoreline erosion and a system for reducing the effects of waves on a shoreline are also provided in accordance with the invention.

Abstract: A dynamic shoreline erosion control system includes a plurality of large rotatable hollow spheres spaced along a line at a distance from the shoreline. The spheres are held together by a heavy duty strong flexible netting which permits movement of the spheres with the incoming and outgoing waves. Openings in the spheres permit the sea water to pass through. A plurality of smaller air-filled balls are disposed within the spheres to likewise rotate under the influence of water flow and provide buoyancy. A group of spheres within a common netting is tied to a pair of pilings at the ends of the netting to limit the extent of movement. Narrower sections of the netting between spheres provide a link and spacing for adjacent elements. Like groups of spheres in nettings can be disposed in parallel pairs with staggered spacing and an array of spaced groups in various geometric arrangements can extend along a large shore area.

Abstract: An air bag module (50) comprising an inflator (40) for generating inflation gas; an air bag (12) disposed about the inflator to receive the inflation gas exiting the inflator; a structure (52) for supporting at least the inflator. The inflator comprises: a pressure vessel (42) and a plurality of exit ports (16). The exit ports are arranged to render the inflator in a thrust neutral condition when the inflator is not attached to the structure. A plug (60) or plugs is provided for closing a designated set of exit ports to render the inflator in a non-thrust neutral condition when supported by the structure. Each plug is received in each of the designated exit ports and is preferably received though a portion of the structure such that if the inflator is removed from the structure each plug must also be removed, thereby opening all of previously closed exit ports and rendering the inflator in its thrust neutral condition once again.

Abstract: A post activation device to indicate that pressure the existed within a pressure vessel just prior to the opening of the pressure vessel and the resulting loss of pressurization, having a diaphragm, plug or piston (62, 130, 160, 330) capable of rupturing, moving or deforming in response to a pressure differential thereacross above one or more low limit values and for providing an indication of the pressure within the pressure vessel just prior to its being opened, and a support member (70), within the pressure vessel for supporting the diaphragm, plug or piston means.

Abstract: An inflator comprising: a pressure vessel (10) for storing a quantity of stored inflation gas under pressure. The pressure vessel comprising a first burst disk (46) and a seal disk (310) both for selectively sealing same. The inflator (10) including a gas release mechanism (52, 300) for permitting the controlled, staged release of the inflation gas at determinable inflation rates. The gas release mechanism includes: a first pyrotechnic device, positioned outside of the pressure vessel and spaced from the first burst disk (46), including a piston (72) moved into a disk piercing position to rupture the first burst disk (46) in response to the buildup of pressure caused burning of a first quantity of propellant material (218).

Abstract: A pressure switch (30) adapted to be placed within a pressurized chamber for generating a logical signal when the pressure within the chamber reduces to a determinable pressure P.sub.1. The pressure switch (30) comprises: a pressure vessel (32) sealed at one end (34) by a snap action diagram (40) and having an inner stop (42) and an outer stop (60, 62) respectively positioned on opposing sides of the diaphragm (40) for limiting diaphragm motion. The outer stop comprises: a first cylindrical portion (78), a first pair of parallel slots (80a, b) formed therein and a through bore (70, 72, 74) extending therethrough. A switch assembly is loosely mounted to the outer stop. The switch assembly (90) comprises an upper housing member (92), a lower housing member (94), first and second electrical contacts (96a, 96b) and a pin (98).

Abstract: An inflator (110) including: a pressure means (12, 14, 16, 20) for storing a quantity of stored inflation gas under pressure. The pressure means including a first burst disk (46) in communication with the stored inflation gas and preventing egress of the gas from the chamber and a hollow sliding piston (172) having at one end thereof cutting edges (174) for puncturing the first burst disk (46). A shear disk (180) having a stationary outer portion (187) and a central portion or second disk (186), central portion is secured to one end of the piston (172) opposite the cutting edges (174). The central portion is capable of separating from the outer portion and moving with the piston (172). A quantity of propellant material (218) is provided near the second burst disk (80) to produce gaseous combustion products when burned.

Abstract: A device (20) for inflating an air bag (37) comprising: a pressure vessel (34) formed by a vessel part (22) and a generator body assembly (30). The generator body assembly is received within opposing openings (24, 26) in the vessel part (22) and secured thereto to provide a gas tight seal (28). The generator body assembly (30) includes: a first bore (38) to receive a quantity of propellant (150) and initiator assembly (120) to cause the propellant (150) to burn in response to a control signal indicative of a vehicle crash. Various passages are provided in the gas generator assembly and oriented to permit the products of combustion of the burning propellant to impinge on inner walls of the pressure vessel to reduce particulate emission.

Abstract: An inflator (10) comprising: a pressure tank (12, 14, 16, 20) for storing a quantity of stored inflation gas under pressure. The pressure tank comprising a first burst disk (46) in communication with the stored inflation gas; a sliding piston (72) including at one end thereof a sharp edge (74) for puncturing the first rupture disk and at its other end a second rupture disk (80). The sliding piston (72) movable into engagement with the first rupture disk in response to the build up of pressure acting on the second rupture disk (80) in response to the burning of propellant material (90) thereby permitting egress of the stored inflation gas. The second burst disk being ruptured by the continued build up of pressure due to the burning propellant whereupon after the rupturing of same the heated products of combustion resulting from the burning propellant flow through the sliding piston (72) to increase the temperature of the stored inflation gas prior to its exit from the inflator.

Abstract: An assembly comprising:a hollow cylindrical member or can including an opening into which a gas generator may be inserted, the gas generator of the type responsive to a control signal, for generating gas to inflate an air bag. The assembly including the air bag, for protecting, when activated, an occupant of a vehicle, comprising an inflatable bag or sack, disposed in a compacted or stored condition, during periods prior to deployment, proximate a side of the cylindrical member containing at least one aperture, the inflatable bag, including an open end disposed about the cylindrical member to receive the gas and including relatively opposing flaps extending from proximate the open end and adapted to envelop the cylindrical member, the flaps, proximate a side of the cylindrical member, away from the at least one aperture, disposed in an overlapping, condition, one to the other. A band of tearable material is provided to secure the air bag in its stored or predeployment condition.

Abstract: A resilient safety extension is provided for highway barriers of the type having a broad base and upstanding central portion with opposed convergently tapered surfaces and a length which is infinitely variable, in the form of an elongated resilient member having an inverted generally U-shaped transverse configuration provided by a top portion and spaced downwardly extending portions adapted to straddle the upstanding portion of the highway barrier, the downwardly extending portions being convergently tapered to provide inner surfaces closely interfitting with the upstanding portion of the barrier and outer surfaces which are slightly divergent in the direction of the top portion, and the thickness of the downwardly extending portions being variable by amounts of the order of 2 inches to provide sinusoidally curved outer surface patterns, repeating at approximately 24" intervals longitudinally of the safety extension.

Abstract: An inflator for an air cushion including a pressure vessel comprising a hollow sleeve for storing a quantity of inert gas such as Argon. One end of the sleeve is closed by a first pyrotechnic actuator assembly while the other end of the sleeve may include a closed end or another pyrotechnic actuator assembly. The pressure vessel is secured within a diffuser which directs inflation gases into the air cushion. The pressure vessel in cooperation with the diffuser defines various regions which assist in reducing the gas turbulence and heat transfer between the gas and the diffuser.

Abstract: An inflator for an air cushion including a pressure vessel comprising a hollow sleeve for storing a quantity of inert gas such as Argon. One end of the sleeve is closed by a first pyrotechnic actuator assembly while the other end of the sleeve may include a closed end or another pyrotechnic actuator assembly. The pressure vessel is secured within a diffuser which directs inflation gases into the air cushion. The pressure vessel in cooperation with the diffuser defines various regions which assist in reducing the gas turbulence and heat transfer between the gas and the diffuser.

Abstract: A shock-absorbing and motorist warning highway barrier is provided comprising a plurality of elongated upstanding units adapted to be joined in end to end relation, each unit comprising an outwardly flared base and an upstanding central portion having interfitting protrusions at opposed ends thereof facilitating end to end coupling of adjacent units, opposed side surfaces of the upstanding portion having a plurality of transversely aligned and longitudinally spaced recesses to receive resilient cylindrical members in a manner to protrude substantially from such surfaces, the cylindrical members being insertable and removable through the top of the unit and being secured in place by a cover member having downward protrusions interfitting with the aligned recesses in said unit. In preferred adaptations each unit may have a sinusoidal contour in the longitudinal dimension incorporating two sine curves, and/or the recesses and cylindrical members mounted therein may be outwardly inclined about 5.degree. to 10.

Abstract: A tissue retraction system includes a dilator and a first retractor member and a retraction assembly. The dilator is configured to be inserted into a tissue body and also includes at least one engagement member. The first retractor member includes a body and at least one engagement member that is configured to attach to the at least one engagement member of the dilator so as to removably attach the retractor member to the dilator body. The retraction assembly includes a retractor body and at least a second retractor member that is movably supported by the retractor body. The retractor body is configured to be attached to the first retractor member. The tissue protection system may also include a neuromonitoring member configured to determine a characteristics of the tissue.