Abstract: The present invention provides methods and compositions for amplifying and sorting adapter tagged nucleic acid fragments using selective primer extension. Immortalized pooled polynucleotide samples and method of producing the same are also provided.

Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.

Abstract: Aspects of the present invention are drawn to methods and compositions for the genetic analysis of regions of interest (ROI) from one or more starting polynucleotide sample(s). In certain aspects, adapter tagged polynucleotide fragments from a plurality of initial polynucleotide samples are pooled, circularized and amplified to produce an immortalized library. Multiple ROI's from this immortalized library are amplified (e.g., in independent iPCR reactions) to generate amplicons, and, in some embodiments, pooled to form a pooled ROI amplicon sample. In certain embodiments, the amplicons for each ROI amplicon in the pooled ROI amplicon sample are present at known molar or mass ratios. The pooled ROI amplicon sample can be analyzed/processed as desired, e.g., sequenced using next generation sequencing technology.

Abstract: A method of “stateful failover” is provided that allows email gateway systems in a cluster to deliver email messages that have been accepted for delivery by a member of the cluster, but has failed with out delivering the messages. The method involves creating a backup copy of the messages that have been accepted for delivery by one email gateway system in the stateful failover cluster on one or more other email gateway systems in the stateful failover cluster. Upon detecting the failure of the email gateway system that accepted the message, another member of the stateful failover cluster that has access to the backup copy of the message queue takes responsibility for the delivery of the messages on the mirrored queue.

Abstract: Disclosed are compositions and methods related to the interaction of polyCUG and polyCCUG repeat RNA and proteins that bind to these repetitive RNA sequences. Also disclosed are methods of treating DM1 or DM2 comprising inhibiting the interaction of poly(CUG)exp or poly(CCUG)exp RNA with muscleblind proteins, or by causing improvement of spliceopathy in myotonic dystrophy.

Abstract: A system and method for recognizing an incoming email as a desired email examines outgoing email messages to arrange the email into fragments for which representations are created and stored. When an incoming message is received, the message is arranged into fragments for which representations are created. The representations of the incoming message are compared to the stored representations and if the matches between stored representations and the representations of the incoming message meet a predefined threshold test, the incoming message is recognized as being desirable. An incoming email message which has been recognized as being desirable can be subjected to a lesser examination to recognize a SPAM message, or to no further examination to recognize a SPAM message.

Abstract: Sensor nodes (or addresses therefore), acting as real-time message decoys, are distributed across a real-time communications network to attract unsolicited real-time messages. Filtering rules are derived from the message characteristics (such as the source address) and messaging content of the traffic encountered at the sensor nodes. The filtering rules are distributed to filtering agents positioned in the communications network in such a way that they can filter traffic for legitimate users. The filtering agents may identify and control the disposition of real-time messaging traffic that is part of a mass communication campaign on behalf of legitimate users of the real-time messaging communication system. Disposition may include suppressing, diverting, or labeling.

Abstract: A method of “stateful failover” is provided that allows email gateway systems in a cluster to deliver email messages that have been accepted for delivery by a member of the cluster, but has failed with out delivering the messages. The method involves creating a backup copy of the messages that have been accepted for delivery by one email gateway system in the stateful failover cluster on one or more other email gateway systems in the stateful failover cluster. Upon detecting the failure of the email gateway system that accepted the message, another member of the stateful failover cluster that has access to the backup copy of the message queue takes responsibility for the delivery of the messages on the mirrored queue.

Abstract: A system and method for recognizing an incoming email as a desired email examines outgoing email messages to arrange the email into fragments for which representations are created and stored. When an incoming message is received, the message is arranged into fragments for which representations are created. The representations of the incoming message are compared to the stored representations and if the matches between stored representations and the representations of the incoming message meet a predefined threshold test, the incoming message is recognized as being desirable. An incoming email message which has been recognized as being desirable can be subjected to a lesser examination to recognize a SPAM message, or to no further examination to recognize a SPAM message.

Abstract: Sensor nodes (or addresses therefore), acting as real-time message decoys, are distributed across a real-time communications network to attract unsolicited real-time messages. Filtering rules are derived from the message characteristics (such as the source address) and messaging content of the traffic encountered at the sensor nodes. The filtering rules are distributed to filtering agents positioned in the communications network in such a way that they can filter traffic for legitimate users. The filtering agents may identify and control the disposition of real-time messaging traffic that is part of a mass communication campaign on behalf of legitimate users of the real-time messaging communication system. Disposition may include suppressing, diverting, or labeling.

Abstract: A method of “stateful failover” is provided that allows email gateway systems in a cluster to deliver email messages that have been accepted for delivery by a member of the cluster, but has failed with out delivering the messages. The method involves creating a backup copy of the messages that have been accepted for delivery by one email gateway system in the stateful failover cluster on one or more other email gateway systems in the stateful failover cluster. Upon detecting the failure of the email gateway system that accepted the message, another member of the stateful failover cluster that has access to the backup copy of the message queue takes responsibility for the delivery of the messages on the mirrored queue.

Abstract: Systems and methods that control a voice mail experience from a client side of a voice mail server system and enhance features thereof. A dynamic voice mail user interface can change based on what predetermined activity the user engages in. Moreover, a field component further enables a caller to identify characteristics of the voice mail. An XML based protocol empowers a client application to control state of the voice mail system, wherein a session Initiation Protocol (SIP) INFO request or response can carry out voicemail control command, result and event.

Abstract: A method and system for configuring a client computer for real-time communication is provided, in which configuration settings are sent to the computer in the form of a configuration document, such as mark-up language document, that is readable by the client computer. The client computer may request the configuration document on an as-needed basis, or may request to be notified whenever new configuration settings are needed.

Abstract: A system and method for recognizing an incoming email as a desired email examines outgoing email messages to arrange the email into fragments for which representations are created and stored. When an incoming message is received, the message is arranged into fragments for which representations are created. The representations of the incoming message are compared to the stored representations and if the matches between stored representations and the representations of the incoming message meet a predefined threshold test, the incoming message is recognized as being desirable. An incoming email message which has been recognized as being desirable can be subjected to a lesser examination to recognize a SPAM message, or to no further examination to recognize a SPAM message.

Abstract: Techniques to allow applications to propagate information to the other instances of the applications running on downstream servers are provided. An indicator, such as a token, tag, etc., is placed within or added to a message that is flowing through the distributed environment to signify that a process has been performed on the message. For example, when an instance of an application running on a server receives a message, the application instance can examine the indicator contained in the message and take appropriate action. If the application instance decides to process the message, the application instance can include an indicator in the message to signify that the application instance processed the message. The presence of the indicator signifies that the message has been processed by an upstream instance of the same application.

Abstract: A method of “stateful failover” is provided that allows email gateway systems in a cluster to deliver email messages that have been accepted for delivery by a member of the cluster, but has failed with out delivering the messages. The method involves creating a backup copy of the messages that have been accepted for delivery by one email gateway system in the stateful failover cluster on one or more other email gateway systems in the stateful failover cluster. Upon detecting the failure of the email gateway system that accepted the message, another member of the stateful failover cluster that has access to the backup copy of the message queue takes responsibility for the delivery of the messages on the mirrored queue.

Abstract: Sensor nodes (or addresses therefore), acting as real-time message decoys, are distributed across a real-time communications network to attract unsolicited real-time messages. Filtering rules are derived from the message characteristics (such as the source address) and messaging content of the traffic encountered at the sensor nodes. The filtering rules are distributed to filtering agents positioned in the communications network in such a way that they can filter traffic for legitimate users. The filtering agents may identify and control the disposition of real-time messaging traffic that is part of a mass communication campaign on behalf of legitimate users of the real-time messaging communication system. Disposition may include suppressing, diverting, or labeling.

Abstract: A game apparatus (10) adapted for use in conjunction with a standard billiard table (16), the game apparatus (10) comprising a sheet of flexible material (12) and at least one target apparatus (14), wherein the or each target apparatus (14) is releasably engageable with the sheet of flexible material (12) and is adapted to releasably receive a ball member (not shown), said game apparatus (10) being adapted to enable the playing of the game in which one or more billiard balls interact with the ball member, the target apparatus (14) and the billiard table (16), in particular cushions and pockets thereof.

Abstract: In a coupling arrangement for freely selectively switching through optical WDM (Wavelength Division Multiplex) signals, the input stage is formed with 1-x-m switching matrices, an optical signal with exactly one wavelength being fed to each 1-x-m switching matrix. In addition, the 1-x-m switching matrices and/or the space division multiplex switches of a central stage connected to the input stage are formed with semiconductor amplifiers (Semiconductor Optical Amplifiers). The coupling arrangement has the capability of switching through individual input signals in a non-blocking fashion to a plurality of output signals (multi-cast capability).

Abstract: In an electric cable or other elongate body, a tubular textile braid comprises two sets of yarns each made up of a plurality of synthetic monofilaments, the yarns of the two sets extending in opposite directions around the axis of the braid corresponding to upper and lower yarns in the braiding machine differing in twist to an extent such that the more twisted yarns have their tensile strength increased by at least 10% but do not have their cover in the braid decreased by more then 25%, both by comparison with an otherwise identical yarn having the same twist of those of the ess twisted set. Preferably the twist in the upper yarns is zero. The invention allows higher production speed and reduced down-time without significant loss of cover, because the small extra twist in the lower yarns gives them better abrasion resistance to withstand passage through their greater exposure in the braiding machine.