Abstract: In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilises ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

Abstract: In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilizes ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

Abstract: This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production scale.

Abstract: The present invention relates to a medium containing non-animal derived proteinaceous material for cultivating patheogenic bacteria to produce an immunogenic factor and processes employing such medium.

Abstract: This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production scale.

Abstract: This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production scale.

Abstract: This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production scale.

Abstract: This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production scale.

Abstract: The present invention relates to a medium containing non-animal derived proteinaceous material for cultivating patheogenic bacteria to produce an immunogenic factor and processes employing such medium.

Abstract: The invention relates to a medium for culturing pathogenic bacteria to produce an immunogenic factor. The medium comprises non-animal derived proteinaceous material. The invention also relates to the use of the medium to cultivate pathogenic bacteria, obtaining immunogenic factors from the bacteria being cultivated and preparing vaccines using the immunogenic factors.

Abstract: The present invention relates to a new method for the growth of Helicobacter pylori and purification of the cytotoxin produced by H. pylori. In particular, H. pylori is cultured in a medium comprising more than 1 gl−1 of glucose to produce a vacuolating cytotoxin.

Abstract: Method for the culture of microorganisms of the genera Helicobacter, Campylobacter and Arcobacter, wherein culture media are employed, which comprise, in place of blood or its derivative, cyclodextrins, methylcellulose or mixtures thereof.

Abstract: Method for the culture of microorganisms of the genera Helicobacter, Campylobacter and Arcobacter, wherein culture media are employed, which comprise, in place of blood or its derivative, cyclodextrins.

Abstract: The process of the invention is useful for the preparation of L-.alpha.-aminoacids and is based on the use of an N-carbamyl-.alpha.-aminoacid compound which is contacted with a E.C.3.5.2.2, a dihydropyrimidine hydrolase, enzyme to form a D-hydantoin. The D-hydantoin is hydrolyzed and racemized to form the D-N-carbamyl-.alpha.-aminoacid and the L-N-carbonyl-.alpha.-aminoacid. The L-N-carbamyl-.alpha.-aminoacid is thereafter transformed into the corresponding L-.alpha.-aminoacid.

Abstract: This invention relates to a process for the preparation of D(-) mandelic acid by the acid hydrolysis of the corresponding carbamate in an aqueous environment at a temperature of between 40.degree. and 100.degree. C. and at a pH of between 1 and 3.5.The invention also relates to a process for the enzymatic hydrolysis of racemic 5-substituted 2,4-oxazolidinediones to give only one of the two possible optical isomers, i.e., the D-carbamyl-.alpha.-hydroxy acid. The free D-.alpha.-hydroxy acid can be obtained from the optically active carbamyl derivative by simple hydrolysis. Of particular interest is the case in which the D-.alpha.-hydroxy acid is D(-)mandelic acid. The enzymatic activity required for preparing the carbamyl derivative of D(-)mandelic acid has been found both in homogenised veal liver and in a series of microorganisms, including Agrobacterium radiobacter, Bacillus brevis, Bacillus stearothermophilus, Pseudomonas sp., Pseudomonas desmolytica, Pseudomonas fluorescens, Pseudomonas putida.

Abstract: The enzyme alpha-galactosidase is prepared from a constitutive mutant of the strain Saccharomyces cerevisiae NRRL-Y-12057, said mutant being marked by the identification number NRRL-Y-12533.

Abstract: Compositions are disclosed, which are adapted to depollute fresh and sea water bodies from crude oil and petroleum product pollution by microbial action. The growth of micro-organisms capable of metabolizing hydrocarbons is exalted by certain combination of nutrients, such as lecithin as a phosphorus source, hydantoins, amides allophanates, polyamines, acyl-ureas and esters of the hydantoic and allantoic acids as the nitrogen sources. Ureido-derivatives of amides are also contemplated as additional nutrients.

Abstract: It has been found that highly active uricase can be produced by fermentation from the microorganism Micrococcus roseus NRRL B 12196. The optimum activity of this microorganism is close to the pH values of the physiological liquors. The uricase so produced can be exploited in the clinical diagnostics for determining uric acid.

Abstract: This invention relates to a process for preparing carbamyl derivatives of .alpha.-hydroxy acids by the hydrolysis of 5-substituted 2,4-oxazolidinediones.The carbamyl derivatives can be further hydrolyzed to .alpha.-hydroxy acids.The invention also relates to a process for the enzymatic hydrolysis of racemic 5-substituted 2,4-oxazolidinediones to give only one of the two possible optical isomers, i.e. the D-carbamyl-.alpha.-hydroxy acid. The free D-.alpha.-hydroxy acid can be obtained from the optically active carbamyl derivative by simple hydrolysis. Of particular interest is the case in which the D-.alpha.-hydroxy acid is D(-)mandelic acid. The enzymatic activity required for preparing the carbamyl derivative of D(-)mandelic acid has been found both in homogenized veal liver and in a series of microorganisms, including Agrobacterium radiobacter, Bacillus brevis, Bacillus stearothermophilus, Pseudomonas sp., Pseudomonas desmolytica, Pseudomonas fluorescens, Pseudomonas putida.

Abstract: A method is disclosed for producing D-aminoacids from racemic mixtures of N-carbamoyl derivatives of such acids or from the corresponding hydantoins wherein the racemic compounds are subjected to the enzymic and microbiological action of an enzymic complex prepared from microorganisms of the Agrobacterium genus, more particularly the NRRL B 11291 strain.