Patents by Inventor Roelof Ary Lans Bovenberg

Roelof Ary Lans Bovenberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11396665
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: July 26, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos, Carsten Pohl, Arnold Jacob Mattieu Driessen, Roelof Ary Lans Bovenberg, Yvonne Irene Nygård
  • Publication number: 20190233477
    Abstract: The present invention relates to a method to improve the production of a secondary metabolite catalyzed by a non-ribosomal peptide synthetase comprising contacting in a eukaryotic host a eukaryotic non-ribosomal peptide synthetase with an MbtH-like protein. The present invention further relates to a composition comprising a eukaryotic non-ribosomal peptide synthetase that is not a hybrid and a prokaryotic MbtH and to a eukaryotic host cell comprising a non-ribosomal peptide synthetase and a polynucleotide allowing the expression of an MbtH-like protein.
    Type: Application
    Filed: April 13, 2017
    Publication date: August 1, 2019
    Applicant: DSM Sinochem Pharmaceuticals Netherlands B.V.
    Inventors: Roelof Ary Lans Bovenberg, Ulrike Maria Muller, Arnold Jacob Mathieu Driessen, Pohl Carsten, Reto Daniel Zwahlen
  • Publication number: 20190194692
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: June 27, 2019
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS, Carsten POHL, Arnold Jacob Mattieu DRIESSEN, Roelof Ary Lans BOVENBERG, Yvonne Irene NYGÅRD
  • Publication number: 20160362715
    Abstract: The present invention relates to the preparation of ?-lactam antibodies comprising contacting 4-hydroxyphenylglycine of phenylglycine, cysteine and valine with a non-ribosomal peptide synthetase and subsequent cyclization using an isopenicillin N synthase in the presence of an MbtH-like protein and to a host cell equipped to perform such preparation.
    Type: Application
    Filed: August 31, 2016
    Publication date: December 15, 2016
    Inventors: Ulrike Maria MUELLER, Rémon BOER, Roelof Ary Lans BOVENBERG
  • Publication number: 20140349340
    Abstract: The present invention relates to the preparation of ?-lactam antibiotics comprising contacting 4-hydroxyphenylglycine or phenylglycine, cysteine and valine with a non-ribosomal peptide synthetase and subsequent cyclization using an isopenicillin N synthase in the presence of an MbtH-like protein and to a host cell equipped to perform such preparation.
    Type: Application
    Filed: January 28, 2013
    Publication date: November 27, 2014
    Inventors: Ulrike Maria Mueller, Rémon Boer, Roelof Ary Lans Bovenberg
  • Publication number: 20140106398
    Abstract: The present invention relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell is a filamentous fungal cell. The invention also relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell comprises a recombinant polynucleotide construct comprising a polynucleotide encoding a biological compound of interest or a compound involved in the synthesis of a biological compound of interest.
    Type: Application
    Filed: March 12, 2012
    Publication date: April 17, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Roelof Ary Lans Bovenberg, Jan Andries Kornelis Willem Kiel, Thibaut José Wenzel, Alrik Pieter Los
  • Patent number: 8293511
    Abstract: The present invention describes a process for the production of an N-?-amino-hydroxyphenylacetyl or an N-?-aminophenylacetyl ?-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl ?-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides.
    Type: Grant
    Filed: October 2, 2007
    Date of Patent: October 23, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Marcus Hans, Roelof Ary Lans Bovenberg, Paul Klaassen, Rémon Boer, Jan Metske Van Der Laan
  • Patent number: 7951568
    Abstract: The present invention discloses a polypeptide selected from the group consisting of: a polypeptide having an amino acid sequence according to SEQ ID NO 3, a polypeptide having an amino acid sequence according to SEQ ID NO 6, a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 3 and a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 6, the polypeptide displaying acetamidase activity and providing a reverse selection on fluoroacetamide with an efficiency of at least 95%, preferably at least 96%, more preferably at least 97%, more preferably at least 98%, more preferably at least 99%, most preferably 100%. The gene encoding the polypeptide of the invention is used as an efficient bi-directional selection marker in the construction of selection marker free strains, in particular for processes for the production of a compound of interest.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: May 31, 2011
    Assignee: DSM IP Assets B.V.
    Inventors: Marco Alexander van den Berg, Roelof Ary Lans Bovenberg
  • Publication number: 20100009404
    Abstract: The present invention describes a process for the production of an N-?-amino-hydroxyphenylacetyl or an N-?-aminophenylacetyl ?-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl ?-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides.
    Type: Application
    Filed: October 2, 2007
    Publication date: January 14, 2010
    Inventors: Marcus Hans, Roelof Ary Lans Bovenberg, Paul Klaassen, Rémon Boer, Jan Metske Van Der Laan
  • Publication number: 20090246826
    Abstract: The present invention discloses a polypeptide selected from the group consisting of: a polypeptide having an amino acid sequence according to SEQ ID NO 3, a polypeptide having an amino acid sequence according to SEQ ID NO 6, a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 3 and a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 6, the polypeptide displaying acetamidase activity and providing a reverse selection on fluoroacetamide with an efficiency of at least 95%, preferably at least 96%, more preferably at least 97%, more preferably at least 98%, more preferably at least 99%, most preferably 100%. The gene encoding the polypeptide of the invention is used as an efficient bi-directional selection marker in the construction of selection marker free strains, in particular for processes for the production of a compound of interest.
    Type: Application
    Filed: April 12, 2007
    Publication date: October 1, 2009
    Inventors: Marco Alexander van den Berg, Roelof Ary Lans Bovenberg
  • Publication number: 20090233287
    Abstract: The present invention provides a recombinant Penicillium chrysogenum strain characterized in that a gene selected from the list consisting of penDE, pcbAB and pcbC is inactivated and the use of such a strain for the preparation of a compound of interest.
    Type: Application
    Filed: April 25, 2007
    Publication date: September 17, 2009
    Inventors: Roelof Ary Lans Bovenberg, Marco Alexander Van den Berg, Susanne Hage, Paul Klaassen, Bernard Meijrink, Lourina Madeleine Raamsdonk
  • Publication number: 20080311631
    Abstract: The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5S,6S]-5,6 dihydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes.
    Type: Application
    Filed: June 2, 2005
    Publication date: December 18, 2008
    Inventors: Marcel Gerhardus Wubbolts, Roelof Ary Lans Bovenberg, Georg Sprenger, Johannes Josef Bongaerts, Stefan Kozak, Michael Muller, Volker Lorbach
  • Patent number: 7402383
    Abstract: The present invention discloses a process for the preparation of variant polynucleotides using a combination of mutagenesis of a starting population of polynucleotides and recombination of the mutated polynucleotides. The process comprises the steps of subjecting a population of polynucleotides to (a series of) two (or more) separate PCR's comprising a first PCR with a forward mutation-specific primer for a position to be mutated and a reverse universal primer and a second PCR with a forward universal primer and a reverse mutation-specific primer for a position to be mutated. The products of the (series of) two (or more) PCR's are assembled by a polymerase, preferably in one tube.
    Type: Grant
    Filed: July 23, 2002
    Date of Patent: July 22, 2008
    Assignee: DSM IP Assets B.V.
    Inventors: Roelof Ary Lans Bovenberg, Richard Kerkman
  • Publication number: 20080131925
    Abstract: The present invention describes the transformation of a microorganism that does not naturally produce a ?-lactam compound with polynucleotides involved in the biosynthesis of ?-lactam compounds and the use of such transformed microorganisms in the production of ?-lactam compounds or in the identification of genes or factors involved in the synthesis of a ?-lactam compound.
    Type: Application
    Filed: December 8, 2005
    Publication date: June 5, 2008
    Inventors: Marco Alexander Van Den Berg, Roelof Ary Lans Bovenberg, Hendrik Jan Noorman, Bastiaan Romein
  • Patent number: 6995003
    Abstract: The invention provides DNA, heterologous host cells capable of transcribing, translating or expressing said DNA and methods employing such host cells and cultures thereof for an improved in vivo production of acylated cephalosporins with higher yield. According to the invention, a host cell is provided comprising an enzyme having expandase activity which is predominantly localized in the cytosol (as opposed to localized mainly in or with the peroxisomes or microbodies) of the host cell.
    Type: Grant
    Filed: May 19, 1999
    Date of Patent: February 7, 2006
    Assignee: DSM IP Assets B.V.
    Inventors: Maarten Nieboer, Roelof Ary Lans Bovenberg
  • Patent number: 6518039
    Abstract: The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.
    Type: Grant
    Filed: May 17, 1999
    Date of Patent: February 11, 2003
    Assignee: DSM N.V.
    Inventors: Maarten Nieboer, Erik De Vroom, Johannis Lugtenburg, Dirk Schipper, Adrianus Wilhelmus Hermanus Vollebregt, Roelof Ary Lans Bovenberg
  • Patent number: 6432672
    Abstract: The present invention relates to filamentous fungi that comprise in their genomes at least two substantially homologous DNA domains which are suitable for integration of one or more copies of a recombinant DNA molecule and wherein at least two of these DNA domains comprise an integrated copy of a recombinant DNA molecule. The invention also relates to methods for preparing such filamentous fungi and for further multiplying the DNA domains with integrated recombinant DNA molecules through gene conversion or amplification.
    Type: Grant
    Filed: January 24, 2000
    Date of Patent: August 13, 2002
    Inventors: Gerardus Cornelis Maria Selten, Bart Willem Swinkels, Roelof Ary Lans Bovenberg
  • Patent number: 6410259
    Abstract: The present invention disclosures a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.
    Type: Grant
    Filed: August 26, 1999
    Date of Patent: June 25, 2002
    Assignee: DSM Patents & Trademarks
    Inventors: Maarten Nieboer, Erik De Vroom, Johannis Lugtenburg, Dirk Schipper, Adrianus Wilhelmus Hermanus Vollebregt, Roelof Ary Lans Bovenberg
  • Patent number: 6368820
    Abstract: An improved process for production of an N-acylated cephalosporin is described. The process utilizes an Acremonium strain which has been modified to express an acyl transferase of a filamentous fungus and is selected for production of the N-acylated cephalosporin at a level which is equal to or higher than the production of the corresponding &agr;-aminoadipyl-7-cephalosporins in the strain.
    Type: Grant
    Filed: January 15, 1999
    Date of Patent: April 9, 2002
    Assignee: DSM N.V.
    Inventors: Roelof Ary Lans Bovenberg, Dirk Schipper, Richard Kerkman
  • Publication number: 20020037547
    Abstract: The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.
    Type: Application
    Filed: May 17, 1999
    Publication date: March 28, 2002
    Inventors: MAARTEN NIEBOER, ERIK DE VROOM, JOHANNIS LUGTENBURG, DIRK SCHIPPER, ADRIANUS WILHELMUS H VOLLEBREGT, ROELOF ARY LANS BOVENBERG