Patents by Inventor Roland Zengerle

Roland Zengerle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170369938
    Abstract: A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.
    Type: Application
    Filed: July 4, 2017
    Publication date: December 28, 2017
    Inventors: Roland ZENGERLE, Felix von Stetten, Guenter Roth, Jochen Hoffmann
  • Patent number: 9725758
    Abstract: A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: August 8, 2017
    Assignee: ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
    Inventors: Roland Zengerle, Felix Von Stetten, Guenter Roth, Jochen Hoffmann
  • Patent number: 9457359
    Abstract: A device for insertion into a rotor of a centrifuge has at least two bodies stacked one above the other in a stacking direction inside a housing insertable into a holder of the rotor of the centrifuge. The two bodies are movably disposed in relation to each other inside the housing in order to fluidically couple, in a first phase, responding to a rotation of the rotor, a first cavity of the first body to a first cavity of the second body and to fluidically couple in a second phase a second cavity of the first body to the first cavity of the second body.
    Type: Grant
    Filed: September 21, 2012
    Date of Patent: October 4, 2016
    Assignees: ALBERT-LUDWIGS-UNIVERSITAET FREIBURG, HAHN-SCHICKARD-GESELLSCHAFT FUER ANGEWANDTE FORSCH
    Inventors: Felix Von Stetten, Marion Finkbeiner, Roland Zengerle, Nils Paust, Guenter Roth
  • Publication number: 20130252796
    Abstract: A device for insertion into a rotor of a centrifuge has at least two bodies stacked one above the other in a stacking direction inside a housing. The housing is configured such that it can be inserted into a holder of the rotor of the centrifuge. With a correct reception of the device in the centrifuge, and upon a rotation of the rotor, a spacing of one of the at least two bodies in relation to the axis of rotation of the rotor is smaller than a spacing of another of the at least two bodies in relation to the axis of rotation of the rotor. A first of the at least two bodies has at least one first cavity and a second cavity and a second body of the at least two bodies has at least one first cavity.
    Type: Application
    Filed: September 21, 2012
    Publication date: September 26, 2013
    Inventors: Felix VON STETTEN, Marion FINKBEINER, Roland ZENGERLE, Nils PAUST, Guenter ROTH
  • Publication number: 20120314528
    Abstract: A device for producing a dilution series from a solution to be diluted, which contains a substance to be diluted, and a dilution solution, includes a body of rotation, a drive configured to subject the body of rotation to rotations having different rotation protocols, and a controller configured to control the drive so as to subject the body of rotation to the different rotational frequencies. A first mixing chamber and a second mixing chamber are connected via a fluidic connection which enables producing, in the first mixing chamber, a first mixture having a first dilution ratio, transferring a partial volume of the first mixture into the second mixing chamber, and there producing a second mixture having a second dilution ratio.
    Type: Application
    Filed: May 11, 2012
    Publication date: December 13, 2012
    Applicant: Albert-Ludwigs-Universitaet Freiburg
    Inventors: Guenter ROTH, Oliver STROHMEIER, Roland ZENGERLE
  • Publication number: 20120015844
    Abstract: A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.
    Type: Application
    Filed: September 2, 2011
    Publication date: January 19, 2012
    Applicants: Albert-Ludwigs-Universitaet Freiburg, Hahn-Schickard-Gesellschaft fuer angewandte Forschung e.V.
    Inventors: Roland ZENGERLE, Felix VON STETTEN, Guenter ROTH, Jochen HOFFMANN
  • Patent number: 7943386
    Abstract: An apparatus for determining the volume fractions of the phases in a suspension includes a body, a channel structure, which is formed in the body, and an inlet area and a blind channel, which is fluidically connected to and capable of being filled via the same. Furthermore, a drive for imparting the body with rotation, so that phase separation of the suspension in the blind channel takes place, is provided. The blind channel includes such a channel cross-section and/or such wetting properties that, when filling same via the inlet area, higher capillary forces act in a first cross-sectional area than in a second cross-sectional area, so that at first the first cross-sectional area fills in the direction from the inlet area toward the blind end of the blind channel and then the second cross-sectional area fills in the direction from the blind end toward the inlet area.
    Type: Grant
    Filed: October 5, 2006
    Date of Patent: May 17, 2011
    Assignee: Albert-Ludwigs-Universitaet Freiburg
    Inventors: Markus Grumann, Jens Ducrée, Roland Zengerle, Lutz Riegger
  • Patent number: 7900850
    Abstract: A microdosing apparatus and method include a fluid conduit having a flexible tube with a first end for connecting to a fluid reservoir and a second end where an outlet opening is located. An actuating device with a displacer with an adjustable stroke is provided, by which the volume of a portion of the flexible tube can be changed to thereby dispense liquid as free flying droplets or as a free flying jet at the outlet opening by moving the displacer between a first end position and a second end position, whereby the tube is partly compressed in the first or the second end position.
    Type: Grant
    Filed: February 14, 2006
    Date of Patent: March 8, 2011
    Inventors: Roland Zengerle, Peter Koltay, Wolfgang Streule, Gerhard Birkle
  • Patent number: 7748962
    Abstract: A fluid handling apparatus includes a body, which comprises a fluid handling structure, and a flexible membrane attached to the body and formed to interact with a fluid in the fluid handling structure, wherein the membrane comprises a first actuation component. A second actuation component is provided, wherein the first and the second actuation component are formed such that the same attract or repel each other in a first positional relationship, in order to actuate the flexible membrane. A driving means is provided to move the body relative to the second actuation component, in order to bring the first and the second actuation component into the first and out of the first positional relationship.
    Type: Grant
    Filed: January 18, 2007
    Date of Patent: July 6, 2010
    Assignee: Albert-Ludwigs-Universitaet Freiburg
    Inventors: Stefan Haeberle, Jens Ducrée, Roland Zengerle, Norbert Schmitt
  • Publication number: 20090197977
    Abstract: A device for producing a mixture of two phases that are insoluble in each other comprises a first fluid channel and a second fluid channel which lead into a contact region. Also, a third fluid channel is provided which leads into the contact region. The device comprises an imparter configured to impart a rotation on the fluid channels, a first phase being centrifugally supplied to the contact region through the first fluid channel, and a second phase, insoluble in the first phase, being supplied to the contact region through the second fluid channel, compressive and/or shearing forces in the contact region which are centrifugally/hydrodynamically induced by the rotation causing drops to break away in one of the phases supplied in order to produce the mixture of the first and second phases.
    Type: Application
    Filed: September 19, 2006
    Publication date: August 6, 2009
    Inventors: Stefan Haeberle, Jens Ducree, Roland Zengerle
  • Patent number: 7479256
    Abstract: A device for device for applying a plurality of microdroplets onto a substrate has a plurality of nozzle orifices in a dosing head. In addition to walls for defining a liquid column of a medium to be dosed on each nozzle orifice, a pressure chamber is provided, which is adapted to be filled with a buffer medium and which is arranged in such a way that said buffer medium can simultaneously be used for applying a pressure to the liquid-column ends which are spaced apart from the nozzle orifices. A pressure generator is provided for applying a pressure to said buffer medium in such a way that a plurality of microdroplets will simultaneously be applied onto the substrate through said plurality of nozzle orifices. Finally, liquid reservoirs for the media to be dosed, which are in fluid communication with the liquid columns on the nozzle orifices are provided.
    Type: Grant
    Filed: April 10, 2000
    Date of Patent: January 20, 2009
    Assignees: Hahn-Schickard-Gesellschaft Fuer Angewandte Forschung E.V.
    Inventors: Holger Gruhler, Nicolaus Hey, Hermann Sandmaier, Roland Zengerle
  • Publication number: 20080280365
    Abstract: An apparatus for determining the volume fractions of the phases in a suspension includes a body, a channel structure, which is formed in the body, and an inlet area and a blind channel, which is fluidically connected to and capable of being filled via the same. Furthermore, a drive for imparting the body with rotation, so that phase separation of the suspension in the blind channel takes place, is provided. The blind channel includes such a channel cross-section and/or such wetting properties that, when filling same via the inlet area, higher capillary forces act in a first cross-sectional area than in a second cross-sectional area, so that at first the first cross-sectional area fills in the direction from the inlet area toward the blind end of the blind channel and then the second cross-sectional area fills in the direction from the blind end toward the inlet area.
    Type: Application
    Filed: October 5, 2006
    Publication date: November 13, 2008
    Applicant: ALBERT-LUDWIGS-UNIVERSIITAT FREIBURG
    Inventors: Markus Grumann, Jens Ducree, Roland Zengerle, Lutz Riegger
  • Patent number: 7418977
    Abstract: A liquid-handling apparatus has a liquid switch, which has a first channel branching into a second and a third channel, wherein the channels are formed in a rotation body. The channels are adapted to route a liquid volume from the first channel into one of the second channel and the third channel dependent on whether a Coriolis force and/or an Euler force prevails a centrifugal force or not. A liquid-handling apparatus having such a liquid switch is particularly suited for applications in which different volumes of liquid have to be passed through a stationary phase.
    Type: Grant
    Filed: October 4, 2004
    Date of Patent: September 2, 2008
    Assignee: Albert-Ludwigs-Universitaet
    Inventors: Jens Ducreé, Roland Zengerle, Thilo Brenner, Thomas Glatzel
  • Patent number: 7396510
    Abstract: A microdosage device comprises a media reservoir used for accommodating a liquid to be dosed, a nozzle connected via a connecting channel to the media reservoir and adapted to be filled via said connecting channel with the liquid to be dosed, and a drive unit for applying, when actuated, to a liquid contained in the media reservoir and in the nozzle a force of such a nature that a substantially identical pressure will be exerted on said liquid contained in the media reservoir and in the nozzle. Flow resistances of the connecting channel and of the nozzle are dimensioned such that, in response to an actuation of the drive unit, a volumetric flow in the connecting channel will be small in comparison with a volumetric flow in the nozzle, said volumetric flow in the nozzle causing an ejection of the liquid to be dosed from an ejection opening of the nozzle.
    Type: Grant
    Filed: January 10, 2002
    Date of Patent: July 8, 2008
    Inventors: Roland Zengerle, Peter Koltay
  • Patent number: 7371330
    Abstract: A particle sedimentation apparatus includes a rotation body rotatable about a rotation axis and a separation chamber formed in the rotation body, to cause sedimentation of particle suspension in the separation chamber upon rotation of the rotation body. The particles accumulate in a radially outward region of the separation chamber. A flow-rate-determining inflow channel having azimuthal path length with an inflow channel inlet and an inflow channel outlet is formed in the rotation body, the inflow channel inlet being arranged radially further inwardly than the inflow channel outlet, the inflow channel outlet being fluidically connected to the separation chamber and formed to introduce particle suspension into the separation chamber upon rotation of the rotation body. Furthermore, an outflow channel is formed in the rotation body with an outflow channel inlet and an outflow channel outlet, to at least partially drain supernatant liquid formed by sedimentation from the separation chamber.
    Type: Grant
    Filed: March 23, 2007
    Date of Patent: May 13, 2008
    Assignee: Albert-Ludwigs-Universitaet Freiburg
    Inventors: Jens Ducree, Roland Zengerle, Stefan Haeberle, Thilo Brenner
  • Publication number: 20070262034
    Abstract: A particle sedimentation apparatus includes a rotation body rotatable about a rotation axis and a separation chamber formed in the rotation body, to cause sedimentation of particle suspension in the separation chamber upon rotation of the rotation body. The particles accumulate in a radially outward region of the separation chamber. A flow-rate-determining inflow channel having azimuthal path length with an inflow channel inlet and an inflow channel outlet is formed in the rotation body, the inflow channel inlet being arranged radially further inwardly than the inflow channel outlet, the inflow channel outlet being fluidically connected to the separation chamber and formed to introduce particle suspension into the separation chamber upon rotation of the rotation body. Furthermore, an outflow channel is formed in the rotation body with an outflow channel inlet and an outflow channel outlet, to at least partially drain supernatant liquid formed by sedimentation from the separation chamber.
    Type: Application
    Filed: March 23, 2007
    Publication date: November 15, 2007
    Inventors: Jens Ducree, Roland Zengerle, Stefan Haeberle, Thilo Brenner
  • Publication number: 20070189910
    Abstract: A fluid handling apparatus includes a body, which comprises a fluid handling structure, and a flexible membrane attached to the body and formed to interact with a fluid in the fluid handling structure, wherein the membrane comprises a first actuation component. A second actuation component is provided, wherein the first and the second actuation component are formed such that the same attract or repel each other in a first positional relationship, in order to actuate the flexible membrane. A driving means is provided to move the body relative to the second actuation component, in order to bring the first and the second actuation component into the first and out of the first positional relationship.
    Type: Application
    Filed: January 18, 2007
    Publication date: August 16, 2007
    Applicant: ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
    Inventors: Stefan Haeberle, Jens Ducree, Roland Zengerle, Norbert Schmitt
  • Publication number: 20060147313
    Abstract: A microdosing apparatus comprises a fluid conduit having a flexible tube with a first end for connecting to a fluid reservoir and a second end where an outlet opening is located. An actuating device with a displacer with adjustable hub is provided, by which the volume of a portion of the flexible hub can be changed to thereby dispense liquid as free flying droplets or as free flying jet at the outlet opening by moving the displacer between a first end position and a second end position, whereby the tube is partly compressed in the first or the second end position. In other words, a microdosing apparatus comprises fluid conduit having a portion along which a cross section of the fluid conduit is variable by an actuating device to effect a change of the volume of the fluid conduit.
    Type: Application
    Filed: February 14, 2006
    Publication date: July 6, 2006
    Inventors: Roland Zengerle, Peter Koltay, Wolfgang Streule, Gerhard Birkle
  • Publication number: 20060073082
    Abstract: A liquid-handling apparatus has a liquid switch, which has a first channel branching into a second and a third channel, wherein the channels are formed in a rotation body. The channels are adapted to route a liquid volume from the first channel into one of the second channel and the third channel dependent on whether a Coriolis force and/or an Euler force prevails a centrifugal force or not. A liquid-handling apparatus having such a liquid switch is particularly suited for applications in which different volumes of liquid have to be passed through a stationary phase.
    Type: Application
    Filed: October 4, 2004
    Publication date: April 6, 2006
    Inventors: Jens Ducree, Roland Zengerle
  • Patent number: 6855293
    Abstract: A fluid management apparatus includes a substrate having a first surface and a second surface opposite to the first surface. A plurality of fluid inlets, which are preferably media reservoirs, are formed in a first pattern in the first surface of the substrate. A plurality of fluid outlets, which are preferably nozzles, are arranged in a second pattern, which is different from the first pattern, in the second surface of the substrate. A plurality of fluid ducts formed in the substrate for connecting respective fluid inlets with respective fluid outlets are provided so that a format conversation from the fluid inlets to the fluid outlets is effected. The fluid outlets have opening cross-sections smaller than that of the fluid inlets. Furthermore, the fluid inlets may be arranged in the raster scheme of microtiter plates.
    Type: Grant
    Filed: March 22, 2000
    Date of Patent: February 15, 2005
    Assignee: Hahn-Schickard-Gesellschaft fuer angewandte Forschung e.V.
    Inventors: Roland Zengerle, Nicolaus Hey, Holger Gruhler, Michael Freygang, Martin Mueller